B3TD46 · B3TD46_CVTKE

Function

function

Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2 (By similarity).
Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses
Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.
Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities (By similarity).
Therefore plays an essential role in viral mRNAs cap methylation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site5551
Active site5553
Active site5652
Active site5728
Active site5733
Binding site5791-5797S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site6206
Active site6221
Active site6261

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell endoplasmic reticulum membrane
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processviral protein processing
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Gene names

    • Name
      1

Organism names

Accessions

  • Primary accession
    B3TD46

Proteomes

Subcellular Location

Cytoplasm
Endoplasmic reticulum membrane
; Multi-pass membrane protein
Host endoplasmic reticulum membrane
; Multi-pass membrane protein
Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1743-1769Helical
Transmembrane1840-1867Helical
Transmembrane1879-1904Helical
Transmembrane2277-2295Helical
Transmembrane2552-2574Helical
Transmembrane2580-2599Helical
Transmembrane2606-2624Helical
Transmembrane2636-2669Helical
Transmembrane3091-3111Helical
Transmembrane3117-3134Helical
Transmembrane3146-3166Helical
Transmembrane3186-3203Helical
Transmembrane3224-3242Helical
Transmembrane3248-3272Helical
Transmembrane3292-3314Helical

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond1798↔1804

Keywords

Interaction

Subunit

Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure (By similarity).
Interacts with ORF6 protein
Homodimer.
Interacts with host PHB and PHB2.
Interacts with papain-like protease and non-structural protein 6.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain675-780Ubiquitin-like
Domain999-1175Macro
Domain1171-1223Ubiquitin-like
Domain1232-1493Peptidase C16
Domain1765-18293Ecto
Region1907-1997Y1
Domain1907-2259CoV Nsp3 Y
Region1911-1924ZF1
Region1957-1967ZF2
Region1998-2259CoV-Y
Region2160-2259Y4
Domain2678-2773Nsp4C
Domain2774-3080Peptidase C30
Domain3374-3456RdRp Nsp7 cofactor
Domain3457-3666RdRp Nsp8 cofactor
Domain3667-3777Nsp9 ssRNA-binding
Domain3779-3920ExoN/MTase coactivator
Domain3934-4192NiRAN
Domain4197-4295Nsp12 Interface
Domain4296-4862Nsp12 RNA-dependent RNA polymerase
Domain4542-4704RdRp catalytic
Domain4863-4946CV ZBD
Domain5119-5471+RNA virus helicase C-terminal
Domain5533-5747ExoN
Domain5756-5983N7-MTase
Region5871-5885GpppA-binding
Domain5984-6044Nsp15 N-terminal oligomerization
Domain6045-6160AV-Nsp11N/CoV-Nsp15M
Domain6177-6318NendoU
Domain6321-6620Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,623
  • Mass (Da)
    742,681
  • Last updated
    2008-09-02 v1
  • Checksum
    8A7B51F149FDB6BE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EU022525
EMBL· GenBank· DDBJ
ABW75124.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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