B3P7F8 · HH_DROER
- ProteinProtein hedgehog
- Genehh
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids465 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Protein hedgehog
The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product) (By similarity).
In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (By similarity).
Once cleaved, the C-product has no signaling activity and diffuses from the cell (By similarity).
In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (By similarity).
Once cleaved, the C-product has no signaling activity and diffuses from the cell (By similarity).
Protein hedgehog N-product
The dually lipidated hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development. Establishes the anterior-posterior axis of the embryonic segments and patterns the larval imaginal disks. Binds to the patched (ptc) receptor, which functions in association with smoothened (smo), to activate the transcription of target genes wingless (wg), decapentaplegic (dpp) and ptc. In the absence of hh, ptc represses the constitutive signaling activity of smo through fused (fu). Essential component of a signaling pathway which regulates the Duox-dependent gut immune response to bacterial uracil; required to activate Cad99C-dependent endosome formation, norpA-dependent Ca2+ mobilization and p38 MAPK, which are essential steps in the Duox-dependent production of reactive oxygen species (ROS) in response to intestinal bacterial infection. During photoreceptor differentiation, it up-regulates transcription of Ubr3, which in turn promotes the hh-signaling pathway by mediating the ubiquitination and degradation of cos.
Catalytic activity
Protein hedgehog
cholesterol + glycyl-L-cysteinyl-[protein] + H+ = [protein]-C-terminal glycyl cholesterol ester + N-terminal L-cysteinyl-[protein]This reaction proceeds in the forward direction.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 143 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 144 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 144 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 149 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 179 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 180 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 180 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 183 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 185 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Site | 251-252 | Cleavage; by autolysis | ||||
Sequence: GC | ||||||
Site | 297 | Involved in cholesterol transfer | ||||
Sequence: D | ||||||
Site | 320 | Involved in auto-cleavage | ||||
Sequence: T | ||||||
Site | 323 | Essential for auto-cleavage | ||||
Sequence: H |
GO annotations
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameProtein hedgehog
- EC number
- Cleaved into 1 chains
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionB3P7F8
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Nuclear up to embryonic stage 10 and then at stage 11 shifts to the cytoplasm. Also secreted in either cleaved or uncleaved form to mediate signaling to other cells.
Protein hedgehog N-product
Cell membrane ; Lipid-anchor
Note: The N-terminal peptide remains associated with the cell surface. Heparan sulfate proteoglycans of the extracellular matrix play an essential role in diffusion. Lipophorin is required for diffusion, probably by acting as vehicle for its movement, explaining how it can spread over long distances despite its lipidation.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for propeptide, signal, lipidation, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Propeptide | PRO_0000383054 | ?-78 | ||||
Sequence: MDNTSSVPWASAASVTCLSLDAKCHSSSAKSAASSISASPETQAMRHIAHTQRCLSRLTSLVALLLIVLPMTFSPAHS | ||||||
Signal | 1-? | |||||
Lipidation | 79 | N-palmitoyl cysteine | ||||
Sequence: C | ||||||
Chain | PRO_0000383056 | 79-251 | Protein hedgehog N-product | |||
Sequence: CGPGRGLGRHRARNLYPLVLKQTIPNLSEYTNSASGPLEGVIRRDSPKFKDLVPNYNRDILFRDEEGTGADRLMSKRCREKLNLLAYSVMNEWPGIRLLVTESWDEDYHHGQESLHYEGRAVTIATSDRDQSKYGMLARLAVEAGFDWVSYVSRRHIYCSVKSDSSISSHVHG | ||||||
Chain | PRO_0000383055 | 79-465 | Protein hedgehog | |||
Sequence: CGPGRGLGRHRARNLYPLVLKQTIPNLSEYTNSASGPLEGVIRRDSPKFKDLVPNYNRDILFRDEEGTGADRLMSKRCREKLNLLAYSVMNEWPGIRLLVTESWDEDYHHGQESLHYEGRAVTIATSDRDQSKYGMLARLAVEAGFDWVSYVSRRHIYCSVKSDSSISSHVHGCFTPESTALLENGVRKPLGELSIGDRVLSMTANGQAVYSEVILFMDRNLEQMQNFVQLHTDGEAVLTVTPAHLVSVWQAESQKLTFVFADRVEEKNQVLVRDVETGELRPQRVVKVGSVRSKGVVAPLTREGTIVVNSVAASCYAVINSQSLAHWGLAPMRLLSTLEAWLPAKEQLHSSPKVVTTAEQQNGIHWYANALYKVKDYVLPQSWRHD | ||||||
Lipidation | 251 | Cholesterol glycine ester | ||||
Sequence: G |
Post-translational modification
Protein hedgehog
The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product) (By similarity).
In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (By similarity).
The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).
In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (By similarity).
The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).
Protein hedgehog N-product
Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
Protein hedgehog N-product
N-palmitoylation by Rasp of the hedgehog N-product, within the secretory pathway, is required for the embryonic and larval patterning activities of the hedgehog signal.
Keywords
- PTM
Interaction
Subunit
Interacts with shf.
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length465
- Mass (Da)51,569
- Last updated2008-09-02 v1
- ChecksumDE0AEEFA4E52362D
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CH954182 EMBL· GenBank· DDBJ | EDV54047.1 EMBL· GenBank· DDBJ | Genomic DNA |