B3P7F8 · HH_DROER

Function

function

Protein hedgehog

The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product) (By similarity).
In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (By similarity).
Once cleaved, the C-product has no signaling activity and diffuses from the cell (By similarity).

Protein hedgehog N-product

The dually lipidated hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development. Establishes the anterior-posterior axis of the embryonic segments and patterns the larval imaginal disks. Binds to the patched (ptc) receptor, which functions in association with smoothened (smo), to activate the transcription of target genes wingless (wg), decapentaplegic (dpp) and ptc. In the absence of hh, ptc represses the constitutive signaling activity of smo through fused (fu). Essential component of a signaling pathway which regulates the Duox-dependent gut immune response to bacterial uracil; required to activate Cad99C-dependent endosome formation, norpA-dependent Ca2+ mobilization and p38 MAPK, which are essential steps in the Duox-dependent production of reactive oxygen species (ROS) in response to intestinal bacterial infection. During photoreceptor differentiation, it up-regulates transcription of Ubr3, which in turn promotes the hh-signaling pathway by mediating the ubiquitination and degradation of cos.

Catalytic activity

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site143Ca2+ 1 (UniProtKB | ChEBI)
Binding site144Ca2+ 1 (UniProtKB | ChEBI)
Binding site144Ca2+ 2 (UniProtKB | ChEBI)
Binding site149Ca2+ 1 (UniProtKB | ChEBI)
Binding site179Ca2+ 1 (UniProtKB | ChEBI)
Binding site180Ca2+ 1 (UniProtKB | ChEBI)
Binding site180Ca2+ 2 (UniProtKB | ChEBI)
Binding site183Ca2+ 2 (UniProtKB | ChEBI)
Binding site185Ca2+ 2 (UniProtKB | ChEBI)
Site251-252Cleavage; by autolysis
Site297Involved in cholesterol transfer
Site320Involved in auto-cleavage
Site323Essential for auto-cleavage

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentendocytic vesicle
Cellular Componentendosome
Cellular Componentextracellular space
Cellular Componentnucleus
Cellular Componentplasma membrane
Molecular Functioncholesterol-protein transferase activity
Molecular Functionmetal ion binding
Molecular Functionmorphogen activity
Molecular Functionpatched binding
Molecular Functionpeptidase activity
Biological ProcessBolwig's organ morphogenesis
Biological Processcell-cell signaling involved in cell fate commitment
Biological Processcytoneme assembly
Biological Processepithelial cell migration, open tracheal system
Biological Processgenital disc anterior/posterior pattern formation
Biological Processgerm cell migration
Biological Processglial cell migration
Biological Processgonadal mesoderm development
Biological Processheart formation
Biological Processhindgut morphogenesis
Biological Processimaginal disc-derived wing morphogenesis
Biological Processintein-mediated protein splicing
Biological Processlabial disc development
Biological Processmorphogenesis of larval imaginal disc epithelium
Biological Processmucosal immune response
Biological Processnegative regulation of homotypic cell-cell adhesion
Biological Processnegative regulation of proteolysis
Biological Processpositive regulation of neuroblast proliferation
Biological Processpositive regulation of protein localization to cell surface
Biological Processprogression of morphogenetic furrow involved in compound eye morphogenesis
Biological Processprotein autoprocessing
Biological Processregulation of cell proliferation involved in compound eye morphogenesis
Biological Processregulation of epithelial cell migration, open tracheal system
Biological Processregulation of mitotic cell cycle
Biological Processsegment polarity determination
Biological Processself proteolysis
Biological Processsmoothened signaling pathway
Biological Processspiracle morphogenesis, open tracheal system
Biological Processterminal cell fate specification, open tracheal system
Biological Processventral midline development
Biological Processwing disc pattern formation

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

Gene names

    • Name
      hh
    • ORF names
      GG12458

Organism names

  • Taxonomic identifier
  • Strain
    • Tucson 14021-0224.01
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    B3P7F8

Proteomes

Subcellular Location

Nucleus
Cytoplasm
Note: Nuclear up to embryonic stage 10 and then at stage 11 shifts to the cytoplasm. Also secreted in either cleaved or uncleaved form to mediate signaling to other cells.

Protein hedgehog N-product

Cell membrane
; Lipid-anchor
Note: The N-terminal peptide remains associated with the cell surface. Heparan sulfate proteoglycans of the extracellular matrix play an essential role in diffusion. Lipophorin is required for diffusion, probably by acting as vehicle for its movement, explaining how it can spread over long distances despite its lipidation.

Keywords

PTM/Processing

Features

Showing features for propeptide, signal, lipidation, chain.

TypeIDPosition(s)Description
PropeptidePRO_0000383054?-78
Signal1-?
Lipidation79N-palmitoyl cysteine
ChainPRO_000038305679-251Protein hedgehog N-product
ChainPRO_000038305579-465Protein hedgehog
Lipidation251Cholesterol glycine ester

Post-translational modification

Protein hedgehog

The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product) (By similarity).
In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (By similarity).
The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).

Protein hedgehog N-product

Cholesterylation is required for N-product targeting to lipid rafts and multimerization.

Protein hedgehog N-product

N-palmitoylation by Rasp of the hedgehog N-product, within the secretory pathway, is required for the embryonic and larval patterning activities of the hedgehog signal.

Keywords

Interaction

Subunit

Interacts with shf.

Family & Domains

Sequence similarities

Belongs to the hedgehog family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    465
  • Mass (Da)
    51,569
  • Last updated
    2008-09-02 v1
  • Checksum
    DE0AEEFA4E52362D
MDNTSSVPWASAASVTCLSLDAKCHSSSAKSAASSISASPETQAMRHIAHTQRCLSRLTSLVALLLIVLPMTFSPAHSCGPGRGLGRHRARNLYPLVLKQTIPNLSEYTNSASGPLEGVIRRDSPKFKDLVPNYNRDILFRDEEGTGADRLMSKRCREKLNLLAYSVMNEWPGIRLLVTESWDEDYHHGQESLHYEGRAVTIATSDRDQSKYGMLARLAVEAGFDWVSYVSRRHIYCSVKSDSSISSHVHGCFTPESTALLENGVRKPLGELSIGDRVLSMTANGQAVYSEVILFMDRNLEQMQNFVQLHTDGEAVLTVTPAHLVSVWQAESQKLTFVFADRVEEKNQVLVRDVETGELRPQRVVKVGSVRSKGVVAPLTREGTIVVNSVAASCYAVINSQSLAHWGLAPMRLLSTLEAWLPAKEQLHSSPKVVTTAEQQNGIHWYANALYKVKDYVLPQSWRHD

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CH954182
EMBL· GenBank· DDBJ
EDV54047.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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