B3MLJ5 · B3MLJ5_DROAN

Function

function

Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.

Catalytic activity

  • (2E)-decenoyl-[ACP] + H+ + NADPH = decanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-dodecenoyl-[ACP] + H+ + NADPH = dodecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-hexadecenoyl-[ACP] + H+ + NADPH = hexadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-hexenoyl-[ACP] + H+ + NADPH = hexanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-octadecenoyl-[ACP] + H+ + NADPH = NADP+ + octadecanoyl-[ACP]
    This reaction proceeds in the forward direction.
  • (2E)-octenoyl-[ACP] + H+ + NADPH = NADP+ + octanoyl-[ACP]
    This reaction proceeds in the forward direction.
  • (2E)-tetradecenoyl-[ACP] + H+ + NADPH = NADP+ + tetradecanoyl-[ACP]
    This reaction proceeds in the forward direction.
  • (3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • 3-oxobutanoyl-[ACP] + H+ + NADPH = (3R)-hydroxybutanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxodecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxydecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxododecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxydodecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxohexadecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyhexadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxohexanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyhexanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxooctadecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyoctadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxooctanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyoctanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxotetradecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxytetradecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • H+ + hexadecanoyl-[ACP] + malonyl-[ACP] = 3-oxooctadecanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • H+ + hexanoyl-[ACP] + malonyl-[ACP] = 3-oxooctanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • H+ + malonyl-[ACP] + octanoyl-[ACP] = 3-oxodecanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • H+ + malonyl-[ACP] + tetradecanoyl-[ACP] = 3-oxohexadecanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • H2O + hexadecanoyl-[ACP] = H+ + hexadecanoate + holo-[ACP]
    This reaction proceeds in the forward direction.
    EC:3.1.2.14 (UniProtKB | ENZYME | Rhea)
  • H2O + tetradecanoyl-[ACP] = H+ + holo-[ACP] + tetradecanoate
    This reaction proceeds in the forward direction.
    EC:3.1.2.14 (UniProtKB | ENZYME | Rhea)
  • a (3R)-hydroxyacyl-[ACP] + NADP+ = a 3-oxoacyl-[ACP] + H+ + NADPH
    This reaction proceeds in the backward direction.
    EC:1.1.1.100 (UniProtKB | ENZYME | Rhea)
  • a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
    EC:4.2.1.59 (UniProtKB | ENZYME | Rhea)
  • a 2,3-saturated acyl-[ACP] + NADP+ = a (2E)-enoyl-[ACP] + H+ + NADPH
    This reaction proceeds in the backward direction.
    EC:1.3.1.39 (UniProtKB | ENZYME | Rhea)
  • a fatty acyl-[ACP] + H+ + malonyl-[ACP] = a 3-oxoacyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
    EC:2.3.1.41 (UniProtKB | ENZYME | Rhea)
  • acetyl-CoA + holo-[ACP] = acetyl-[ACP] + CoA
    This reaction proceeds in the forward direction.
    EC:2.3.1.38 (UniProtKB | ENZYME | Rhea)
  • acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H+ = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+.
    EC:2.3.1.85 (UniProtKB | ENZYME | Rhea)
  • acetyl-[ACP] + H+ + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • butanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxohexanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • decanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxododecanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • dodecanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxotetradecanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site912Proton acceptor; for dehydratase activity
Active site1063Proton donor; for dehydratase activity

GO annotations

AspectTerm
Molecular Function(3R)-3-hydroxybutanoyl-[acyl-carrier-protein] hydratase activity
Molecular Function(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity
Molecular Function(3R)-3-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity
Molecular Function(3R)-3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity
Molecular Function(3R)-3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity
Molecular Function3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Function3-oxoacyl-[acyl-carrier-protein] synthase activity
Molecular Function[acyl-carrier-protein] S-acetyltransferase activity
Molecular Functionenoyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Functionfatty acid synthase activity
Molecular Functionfatty acyl-[ACP] hydrolase activity
Molecular Functionphosphopantetheine binding
Biological Processcellular response to sucrose stimulus
Biological Processfatty acid biosynthetic process
Biological Processglycogen metabolic process
Biological Processintracellular triglyceride homeostasis
Biological Processregulation of lipid storage
Biological Processtriglyceride biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Gene names

    • Name
      Dana\GF14401
    • Synonyms
      dana_GLEANR_15164
    • ORF names
      GF14401

Organism names

  • Taxonomic identifier
  • Strain
    • Tucson 14024-0371.13
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    B3MLJ5

Proteomes

PTM/Processing

Keywords

Interaction

Protein-protein interaction databases

Structure

3D structure databases

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain38-445Ketosynthase family 3 (KS3)
Region880-999N-terminal hotdog fold
Domain880-1146PKS/mFAS DH
Region1014-1146C-terminal hotdog fold
Domain2046-2132Carrier
Region2121-2155Disordered
Compositional bias2133-2155Polar residues

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,441
  • Mass (Da)
    266,853
  • Last updated
    2016-01-20 v2
  • Checksum
    230D433D72AF9010
MPARFAEEVITAEPAQRAAPQLDLGGGHYAPRQQQQLNDEIAITGFSGRLPESSTIEEFKQNLFNGVDMVNDDPRRWERGLYGLPDRIGKIKEEDLENFDQQFFGVHQKQAECMDPLLRMLLELTHEAIIDAGLNPSDLRGSRTGVYIGVSNSETEQHWCSDPDRVNGYGLTGCARAMFANRISFTFDFKGPSYSIDTACSSSLYALEQAFSDMRQGKIDNALVAGAGLILKPTMSLQFKRLNMLSQDGSCKAFDESGNGYVRSDGCVVLLLQRTSTARRVYASILNVRTNTDGFKEQGITYPIGKMQNRLIRETYEEIGLNPNEVVYVEAHGTGTKVGDPQEVNSITDFFCKNRSNPLLIGSVKSNMGHSEPASGVCSVAKILIAMEEGVIPGNLHYNKPNPDLYGLLDGRLKVVDKNLPWNGGIIGLNSFGFGGANAHVILKSNPKPKALTPKDGAAKVVVASGRTFEAVEQLLESAANHADDDEYLQLINDIHSKTIPNHYFRGYGVVSSKGTHQREVLEYSDEKRPVWYIYSGMGSQWASMAKDLMKIEVFAKSIQRCADILKPEGVDLIDVLTRSTDKSFENILNSFISIAAMQVALTDLLSSLGITPDGIVGHSVGELGCAYADGCFTPEQTVLAAYWRGKSILDTQLAKGKMAAVGLSWEDAHQRVPADCFPVCHNSEDNCTISGPEASIEALVAKLNVEGVFAKAVNSSGYAFHSKYIADAGPKLRKSLEKIIPNAKNRTTRWISTSIPESAWNSPVAKQSSAAYHVNNLLSPVLFHEALQHVPKNAISVEIAPHGLLQAILKRALGPDATNLSLVKRGHENNVEFFLTNVGKLYAAGAQPQVLNLVRPISYPVGRGTPMLNSKVGWDHTQKWLVAKFGKETSSGETIVEVDLSKEDDAFLAGHTIDGRILFPATGYMTLAWQTFAKMRGSEFHKTPVVMENLVFHRATILNKNAVVKFGINFFDGTGAFEICESGSLAVSGKITIPESIENEELPLEAQTPSTVAKELVTGDVYKELRLRGYDYAGIFRGIVRSDTVASTGTLQWVDNWISFMDTMLQFSILSKNLRELYLPTRIERAVINPLKHLELLSGLTKEQQLETGVPVQWYSDINVIKSGGVELRGLKANLAQRRPGTQAPPTLERYQFVPNINTTDLNENSEKARLHALDVAIQLIIENSSGAVKLKGVELANGRNPDVLVANRLLQIIEGEPVLTGDVAVVTSNNNEETITAALGDSGVRVVSKDVLKEPVEQNCHFVFGIDVLSRPDTKTLENSIATIRENGFLIFEESVSSYTKTGRALLAKFGFVAVQEQSLGASRVLVLARKTVDLKTRKSVVVVATEQNFTWVDDLKAALATAQTEEQYVYVVCQGEELFGAVGLMTCIKNENGGKLARLVFVQDTKAEKFSLTSALYRQQLEKDLISNVLKNGAWGTFRHLKLETQQATLQVEHAYVNALVKGDLASLKWIEAAQAQTVSANKDLETCTVYSAPINFRDVMLTSGKLAADALPGDLAEQDCVLGLEFAGRDSQGRRVMAMVPAKSLATTCVASKRMMWQIPEKWSMEEASTVPCVYSTVYYALVVRGQMKKGEKILIHAGSGGVGQAAISVALAHGLTVFTTVGSKEKREFLLKRFPKLQERNIGNSRDTSFEQLVMRETKGRGVDLVLNSLSEEKLQASIRCLGLNGRFLEIGKFDLSNNNPLGMSVFLKNTSFHGILLDSVMEGEEEMQNQVVSLVAEGIKSGAVVPLPTSVFNDQQVEQAFRFMASGKHIGKVVIKVRDEEPGKKALQPKSRLINAIPRTYMHPEKSYILVGGLGGFGLELTNWLVSRGARFIVLTSRSGVKTGYQGLMIRRWQERGVKVVIDTSDVTTAAGAKKLLENSNKLALVGGIFNLAAVLRDALIEDQTAKDFKTVADPKVTATKYLDQYSRTICTELDYFICFSSVSCGRGNIGQTNYGLANSAMERICEQRQVSGFPGTAIQWGAIGDTGLVLENLGDNDTVIGGTLPQRMPSCLQTIDLFLQQPHPVVASMVVAEKRKSDQSGGVSLIATIANILGLRDTKNIQDGASLADLGMDSLMSAEIKQTLERNFDIVLSAQEIRGLTFGALKAMDGGAPPAAAAAPAASAPATGTGATTELNITSGGSSRTASPLGDGTQVVFTKELIPTQTIVQLATKAAATSKQTPIFFVSPIEGFASALEPLAKRLEVPAYGLQFTEAVPLDSVDSAARFFIKQLRTVQPKGPYKLAGYSFGCLLTYVMAAILEETNEVANVIMLDGAPTYVNWYTSSFKQRYTADDDNNNQSYGLAYFGIVLANIDYKALVRLLLVIPTWEEKLERFAELMSNEITQPVEVIKKSATLFYKKLELADSYKPTLKLKSIVSLVKPTDNSAKLDEDYRLKEVCTKPVEVHTVEGNHRTFLIEDQSLTTIQSILKRLFN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias2133-2155Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CH902620
EMBL· GenBank· DDBJ
EDV31744.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp