B3LF48 · EHD2_ARATH

Function

function

Involved in endocytosis negative regulation, probably by influencing actin organization. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Exhibits an inhibitory effect on endocytosis when over-expressed.

Catalytic activity

Features

Showing features for binding site.

154650100150200250300350400450500
TypeIDPosition(s)Description
Binding site28Ca2+ 1 (UniProtKB | ChEBI)
Binding site30Ca2+ 1 (UniProtKB | ChEBI)
Binding site32Ca2+ 1 (UniProtKB | ChEBI)
Binding site34Ca2+ 1 (UniProtKB | ChEBI)
Binding site39Ca2+ 1 (UniProtKB | ChEBI)
Binding site62Ca2+ 2 (UniProtKB | ChEBI)
Binding site73Ca2+ 2 (UniProtKB | ChEBI)
Binding site204-211GTP (UniProtKB | ChEBI)
Binding site292-296GTP (UniProtKB | ChEBI)
Binding site359GTP (UniProtKB | ChEBI)
Binding site395-398GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentendosome membrane
Cellular Componentintracellular membrane-bounded organelle
Cellular Componentmembrane
Cellular Componentnucleus
Cellular Componentplasma membrane
Cellular Componentplasmodesma
Molecular Functioncalcium ion binding
Molecular FunctionG protein activity
Molecular FunctionGTP binding
Biological Processendocytosis
Biological Processprotein homooligomerization
Biological Processregulation of actin cytoskeleton organization

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    EH domain-containing protein 2
  • EC number
  • Short names
    AtEHD2

Gene names

    • Name
      EHD2
    • ORF names
      T1J24.10
    • Ordered locus names
      At4g05520

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    B3LF48
  • Secondary accessions
    • F4JGH2
    • Q9S9W1

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Endosome membrane
; Peripheral membrane protein
Cell membrane
; Peripheral membrane protein
Nucleus
Cytoplasm

Keywords

Phenotypes & Variants

Disruption phenotype

Early flowering in short-day growth conditions.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis37Slighty increased nuclear subcellular localization.
Mutagenesis204Loss of negative effect on endocytosis.
Mutagenesis488-546Loss of negative effect on endocytosis.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 44 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004318061-546EH domain-containing protein 2

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Homooligomer, and heterooligomer with EHD1 (PubMed:18547399).
Interacts with AP-4 complex subunit sigma (At2g19790) (PubMed:19936242).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region, motif, coiled coil.

TypeIDPosition(s)Description
Domain15-50EF-hand 1
Domain16-94EH
Domain51-84EF-hand 2
Domain194-430Dynamin-type G
Region204-211G1 motif
Region230-231G2 motif
Region292-295G3 motif
Region358-361G4 motif
Region382G5 motif
Motif429-436Nuclear localization signal
Coiled coil467-490

Domain

The coiled coil domain (488-546) is required to inhibit endocytosis.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

B3LF48-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    546
  • Mass (Da)
    61,383
  • Last updated
    2011-05-03 v1
  • Checksum
    B78F38337D934B6E
METSSTISIGSCLKEHQKIYKEWFNIADSDGDGRVSGNDATKFFAMSKLSRQELKQVWAVADSKRQGFLGLSEFITAMKLVSLAQEGHEITSDLLKGSIDMKSVELPVLEGLENVVSKQKVSKTNVDVEDNVVTKPQVTAKTPWFKSKSIIKPQVNVVTIVDGLKRLYTEKLKPLEVTYRFNDFASPVLTSSDFDAKPMVMLLGQYSTGKTTFIKHLLGCDYPGAHIGPEPTTDRFVVAMSGPDERTIPGNTMAVQADMPFNGLTSFGGAFLSKFECSQMPHPVLDQITLVDTPGVLSGEKQRMQRSYDFTGVISWFASKCDMILLLFDPHKLDISDEFKRVITSLRGNEDKIRVVLNKADQVDTQQLMRVYGALMWSLGKVLNTPEVVRVYIGSFNDKPINEVAVGPIGKELFEKEQNDLLADLMDVPKKACDRKINEFVKRARSAKINAYIMSHLKKEMPAMMGKSKAQQRLMDNLEEEFGKVQREFHLPAGDFPSVEHFREVLGGYNIDKFEKLKPKMIQAVDDMLGYDIPDLLKKFRNPYDN

B3LF48-2

Sequence caution

The sequence AAD48968.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence CAB81094.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_057385132in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF147263
EMBL· GenBank· DDBJ
AAD48968.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AL161503
EMBL· GenBank· DDBJ
CAB81094.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002687
EMBL· GenBank· DDBJ
AEE82529.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002687
EMBL· GenBank· DDBJ
AEE82530.1
EMBL· GenBank· DDBJ
Genomic DNA
BT033036
EMBL· GenBank· DDBJ
ACE79044.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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