B3LF48 · EHD2_ARATH
- ProteinEH domain-containing protein 2
- GeneEHD2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids546 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in endocytosis negative regulation, probably by influencing actin organization. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Exhibits an inhibitory effect on endocytosis when over-expressed.
Catalytic activity
- GTP + H2O = GDP + H+ + phosphate
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 28 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 30 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 32 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 34 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 39 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 62 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 73 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 204-211 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GQYSTGKT | ||||||
Binding site | 292-296 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DTPGV | ||||||
Binding site | 359 | GTP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 395-398 | GTP (UniProtKB | ChEBI) | ||||
Sequence: SFND |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | endosome membrane | |
Cellular Component | intracellular membrane-bounded organelle | |
Cellular Component | membrane | |
Cellular Component | nucleus | |
Cellular Component | plasma membrane | |
Cellular Component | plasmodesma | |
Molecular Function | calcium ion binding | |
Molecular Function | G protein activity | |
Molecular Function | GTP binding | |
Biological Process | endocytosis | |
Biological Process | protein homooligomerization | |
Biological Process | regulation of actin cytoskeleton organization |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEH domain-containing protein 2
- EC number
- Short namesAtEHD2
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionB3LF48
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Endosome membrane ; Peripheral membrane protein
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Early flowering in short-day growth conditions.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 37 | Slighty increased nuclear subcellular localization. | ||||
Sequence: G → R | ||||||
Mutagenesis | 204 | Loss of negative effect on endocytosis. | ||||
Sequence: G → R | ||||||
Mutagenesis | 488-546 | Loss of negative effect on endocytosis. | ||||
Sequence: Missing |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 44 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000431806 | 1-546 | EH domain-containing protein 2 | |||
Sequence: METSSTISIGSCLKEHQKIYKEWFNIADSDGDGRVSGNDATKFFAMSKLSRQELKQVWAVADSKRQGFLGLSEFITAMKLVSLAQEGHEITSDLLKGSIDMKSVELPVLEGLENVVSKQKVSKTNVDVEDNVVTKPQVTAKTPWFKSKSIIKPQVNVVTIVDGLKRLYTEKLKPLEVTYRFNDFASPVLTSSDFDAKPMVMLLGQYSTGKTTFIKHLLGCDYPGAHIGPEPTTDRFVVAMSGPDERTIPGNTMAVQADMPFNGLTSFGGAFLSKFECSQMPHPVLDQITLVDTPGVLSGEKQRMQRSYDFTGVISWFASKCDMILLLFDPHKLDISDEFKRVITSLRGNEDKIRVVLNKADQVDTQQLMRVYGALMWSLGKVLNTPEVVRVYIGSFNDKPINEVAVGPIGKELFEKEQNDLLADLMDVPKKACDRKINEFVKRARSAKINAYIMSHLKKEMPAMMGKSKAQQRLMDNLEEEFGKVQREFHLPAGDFPSVEHFREVLGGYNIDKFEKLKPKMIQAVDDMLGYDIPDLLKKFRNPYDN |
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain, region, motif, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 15-50 | EF-hand 1 | ||||
Sequence: EHQKIYKEWFNIADSDGDGRVSGNDATKFFAMSKLS | ||||||
Domain | 16-94 | EH | ||||
Sequence: HQKIYKEWFNIADSDGDGRVSGNDATKFFAMSKLSRQELKQVWAVADSKRQGFLGLSEFITAMKLVSLAQEGHEITSDL | ||||||
Domain | 51-84 | EF-hand 2 | ||||
Sequence: RQELKQVWAVADSKRQGFLGLSEFITAMKLVSLA | ||||||
Domain | 194-430 | Dynamin-type G | ||||
Sequence: FDAKPMVMLLGQYSTGKTTFIKHLLGCDYPGAHIGPEPTTDRFVVAMSGPDERTIPGNTMAVQADMPFNGLTSFGGAFLSKFECSQMPHPVLDQITLVDTPGVLSGEKQRMQRSYDFTGVISWFASKCDMILLLFDPHKLDISDEFKRVITSLRGNEDKIRVVLNKADQVDTQQLMRVYGALMWSLGKVLNTPEVVRVYIGSFNDKPINEVAVGPIGKELFEKEQNDLLADLMDVPK | ||||||
Region | 204-211 | G1 motif | ||||
Sequence: GQYSTGKT | ||||||
Region | 230-231 | G2 motif | ||||
Sequence: EP | ||||||
Region | 292-295 | G3 motif | ||||
Sequence: DTPG | ||||||
Region | 358-361 | G4 motif | ||||
Sequence: NKAD | ||||||
Region | 382 | G5 motif | ||||
Sequence: V | ||||||
Motif | 429-436 | Nuclear localization signal | ||||
Sequence: PKKACDRK | ||||||
Coiled coil | 467-490 | |||||
Sequence: KSKAQQRLMDNLEEEFGKVQREFH |
Domain
The coiled coil domain (488-546) is required to inhibit endocytosis.
Sequence similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
B3LF48-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length546
- Mass (Da)61,383
- Last updated2011-05-03 v1
- ChecksumB78F38337D934B6E
B3LF48-2
- Name2
- Differences from canonical
- 132-132: Missing
Sequence caution
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_057385 | 132 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF147263 EMBL· GenBank· DDBJ | AAD48968.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AL161503 EMBL· GenBank· DDBJ | CAB81094.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002687 EMBL· GenBank· DDBJ | AEE82529.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE82530.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT033036 EMBL· GenBank· DDBJ | ACE79044.1 EMBL· GenBank· DDBJ | mRNA |