B3KY32 · B3KY32_HUMAN
- ProteinUDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids408 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transfering GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.
Catalytic activity
- a di-trans,poly-cis-dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UMPThis reaction proceeds in the forward direction.
a di-trans,poly-cis-dolichyl phosphate RHEA-COMP:19498 + CHEBI:57705 = an N-acetyl-α-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol RHEA-COMP:19507 + CHEBI:57865
Cofactor
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | glycosyltransferase activity | |
Molecular Function | UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity | |
Biological Process | dolichol-linked oligosaccharide biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
- EC number
- Alternative names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionB3KY32
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 12-32 | Helical | |||
Transmembrane | 60-83 | Helical | |||
Transmembrane | 95-114 | Helical | |||
Transmembrane | 126-146 | Helical | |||
Transmembrane | 167-189 | Helical | |||
Transmembrane | 195-211 | Helical | |||
Transmembrane | 223-243 | Helical | |||
Transmembrane | 249-271 | Helical | |||
Transmembrane | 379-401 | Helical | |||
Keywords
- Cellular component
PTM/Processing
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length408
- Mass (Da)46,118
- Last updated2008-09-02 v1
- Checksum627087E6DCE7AB45
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK128572 EMBL· GenBank· DDBJ | BAG54694.1 EMBL· GenBank· DDBJ | mRNA |