B3KMX0 · B3KMX0_HUMAN
- ProteinDNA replication licensing factor MCM4
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids863 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+This reaction proceeds in the forward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | MCM complex | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA helicase activity | |
Biological Process | DNA replication initiation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA replication licensing factor MCM4
- EC number
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionB3KMX0
Subcellular Location
PTM/Processing
Interaction
Subunit
Component of the MCM2-7 complex.
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-60 | Disordered | |||
Compositional bias | 44-60 | Polar residues | |||
Region | 91-126 | Disordered | |||
Domain | 458-666 | MCM | |||
Sequence similarities
Belongs to the MCM family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length863
- Mass (Da)96,604
- Last updated2008-09-02 v1
- MD5 Checksum99FAC5931912B4D35BF7B44706A35A52
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 44-60 | Polar residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK022899 EMBL· GenBank· DDBJ | BAG51132.1 EMBL· GenBank· DDBJ | mRNA |