B3FHU4 · B3FHU4_CVTKE

Function

function

Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2 (By similarity).
Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses
Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.
Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities (By similarity).
Therefore plays an essential role in viral mRNAs cap methylation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
    EC:3.4.19.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell endoplasmic reticulum membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular Functiontransferase activity
Molecular Functionzinc ion binding
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processviral genome replication
Biological Processviral protein processing
Biological Processviral translational frameshifting

Keywords

Names & Taxonomy

Protein names

  • Submitted names
    • ORF1a

Gene names

    • Name
      1a

Organism names

Accessions

  • Primary accession
    B3FHU4

Proteomes

Subcellular Location

Cytoplasm
Endoplasmic reticulum membrane
; Multi-pass membrane protein
Host endoplasmic reticulum membrane
; Multi-pass membrane protein
Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1747-1763Helical
Transmembrane1844-1863Helical
Transmembrane1870-1888Helical
Transmembrane1894-1912Helical
Transmembrane2281-2299Helical
Transmembrane2558-2579Helical
Transmembrane2586-2606Helical
Transmembrane2612-2630Helical
Transmembrane2642-2667Helical
Transmembrane3096-3116Helical
Transmembrane3123-3142Helical
Transmembrane3154-3183Helical
Transmembrane3190-3209Helical
Transmembrane3263-3285Helical
Transmembrane3297-3320Helical

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond1802↔1808

Keywords

Interaction

Subunit

Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure (By similarity).
Interacts with ORF6 protein
Homodimer.
Interacts with host PHB and PHB2.
Interacts with papain-like protease and non-structural protein 6.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain675-780Ubiquitin-like
Domain1005-1181Macro
Domain1177-1229Ubiquitin-like
Domain1238-1499Peptidase C16
Domain1769-18333Ecto
Region1911-2001Y1
Domain1911-2263CoV Nsp3 Y
Region1915-1928ZF1
Region1961-1971ZF2
Region2002-2263CoV-Y
Region2164-2263Y4
Domain2684-2779Nsp4C
Domain2780-3086Peptidase C30
Domain3380-3462RdRp Nsp7 cofactor
Domain3463-3672RdRp Nsp8 cofactor
Domain3673-3783Nsp9 ssRNA-binding
Domain3785-3926ExoN/MTase coactivator

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    3,951
  • Mass (Da)
    440,652
  • Last updated
    2008-07-22 v1
  • Checksum
    8051C3E28F720572
MASSLKQGVSPKPRDVILVSKDIPDQLCDALFFYTSHNPKDYADAFAVRHKFDRNLQTGKQFKFETVCGLFLLKGVDKITPGVPAKVLKATSKLADLEDIFGVSPFARKYRELLKTACQWSLTVDTLDARAQTLDEIFDPTEIFWLQVAAKVQVSAMAMRRLVGEVTAKVMEALGSNLSVLFQIVKQQIARIFQKALAIFENVSDLPQRVAALKMAFAKCAKSITVVVVERTLVVREFAGTCLASINGAVAKFFEELPNGFMGSKVFTTLAFFKEAAVKIVENIPNAPRGTKGFEVIGNAKGTQVVVRGMRNDLTLLDQKADVPVESEGWSAILEGHLCYVFKSGDRFYAAPLSGNFALHDVHCCERVVCLSDGVTPEINDGLVLAAIYSSFGVSELVAALKKGEPFKFLGHKFVYVKDEAVSFTLAKAATIADVLKLFQSARVQVDDVWSSFTEKSFEFWRRAYGKVRHLEELVKTHFCKAQMSIVVLAAVLGEGIWHLASQVIYKLGGLFTKVVDFCEKHWQGFCVQLKRAKLVVTENFCVLKGVAQHCFQLLLGAIHSLYKSFKKCVLGRIHGDLLFWKGGVHKIVQDGDEIWFDAIDSVDVEDLGVVKDKPIDFEVCEDVTLPENQPGHMVQIEEDGKNYMFFRFKRDENIYYTPMSQLGAINVVCKAGGKTVTFGGTTVKEIPPPDVVPIKVSIECCGEPWNTIFKKAYKEPIEVETDLTVEQLLSVIYEKMCDDLKLFPEAPEPPPFENVALVDKNGKDLDCIKSCHLIYRDYESGDDIEEDDAEEFDTDSADAEECDTASECEEEDEDTKVLALIQDPASNKYPLPLDDDYSVYNGAIVHKDALDVVNLPSGEETFVVSNCFEGAVKPLPQKVIDVLGDWGEAVDAQEQLCQQESTRVVFEKPVEDSTGSCEAMVERTVVEEHAVVPVVENKSDVVVYTPADLEVVKETAEEVDEFILISDVSKEEVVSQEKEEPQVEQHPTQVVKPQREKKAKKFKVKPATCEKPKFLEYKTCVGDLTVVIAKALDEFKEFCIVNAANEHMSHGGGVAKAIADFCGPDFVEYCEDYVKKHGPQQRLVTPSFVKGIQCVNNVVGPRHGDSNLREKLVAAYKKVLVDGVFNYVVPVLSSGIFGVDFKMSIDAMREAFDGCDIRVLLFSLSPEHIDYFNATCKQKTLYLTEDGVKYRSIVVKPGDSLIQFRQVFAKNKAVFTADDVEDKEILFVPTTDKTVLEYYHLDAQKYVIYLQTLAQKWNVQYRDNFIILEWRDGNCWVSSVAVLLQAAKIRFRGFLAEAWAKFLGGDPTEFVAWCYASCNAKVGDFSDTNWLLANLAEHFDADYTNALLKKRVSCNCGVKSYELRGLEACIQPVKAPNLLHFKTQYSNCSICGANSTDEVIEASLPYLLLFATDGPAAVDCDENAVGTVVFIGSTNSGHCYTQAIDKAFDNLAKDRKFGKKSPYITAMYMRFSLKSENPLPVVKQSKGKTKVVKEDVSNLATSSKVSFDDLTDFEHWYDSNIYESLKVQETPDTFDGYVSFTTKEDSKLPLTLKVRGIKSAVDFRSKDGFTYKLTPDTDENSKAPVYYPVLDAISLKAIWVEGNANFVVGHPNCYSKSLRIPTFWESAESFFKIGDKIDGVTMGLWRAEHLNKPNLERIFNIAKKAIVGSSVVTTQCGKLISKAATFIADKVGGGVVRNITDSIKGFFGFTRGHFELSPQFIKTLIFFFFYFIKASAKSLVSSYKRVLCKVVFATLLIVWFIYTSNPVIFTGIRVLDFLVEGSLCGPYNDYGKDSFDVLRYCADDFTCRVCLHDRDSLHLYKHAYSVEQIYKAASSGIVFNWNWLYLVFLILFVKPVAGFVIICYCVKYLVLNSTVLLTGVGFLDWFVQTVFNHFNFMGAGFYFWLFYKIYIQVYHILYCKDVTCEVCKRVARSNRQEVSVVVGGRKQIVHVYTNSGYKFCKKHNWYCRNCDDYGHQNTFMSPEVAGELSEKLKRHVKPTAYAYHVVDEACLVDDFVNLKYKAAIPGKDSAPLAVKCFSITDFLKKAVFLKDALKCEQISNDGFIVCNTQSAHALEDAKNAAIYYAQYLCKPILLLDQVLYEQLVVEPVSKSVVDKVCSILSNIISVDTAALNYKAGTLRDALLSITKDEEAVDMAIFCHNHDVEYTSDGFTNVIPSYGIDTDKLTPRDRGFLINADASIANLRVKNAPPVVWKFSDLIKLSDSCLKYLISATVKSGVRFFITKSGAKQVISCHTQKLLVEKKAGGIIGGTFNWFKSCCKWLLIFYVLFTLCCLGYYHMEMNKIFVHPMYDVNSTMHVEGFKVIDKGVIRDIVPEDACFSNKFANFDAFWGKSYENSRDCPIVTAIIDGAGTVAAGVPGFVDWVMNGVMFVHMAQTDRRPWYVPAWFNREIVGYTQDSIVTEGSFYTSIALFSARCLYLIASNTPQLYCFNGDSDAPGALPFSSIIPHRVYFQPNGVRLIVPQQIMHTPYIVKLLSDSYCRGSVCEYTKPGYCMSLNSQWVLFNDEYIGRPGVFCGSTVRELVFNMVSTFFTGVNPNIYMQLATMFLILVIVVLIFAMVIKFQGVFKAYATIVFTIMLVWAVNAFVLCVHSYNSVVAAILLVLYCYASLVTSRNTSIIMHCWLVFTFGLIVPIWLACCYLAFVLYVYTPLFFWCYGTTKNTRKLYDGNEFVGNYDLAAKSTFVIRGSEFVKLTNEIGDKFEAYLSAYARLKYYSGTGSEQDYLQACRAWLAYALDQYRNSGVEIVYTPPRYSIGVSRLQAGFKKLVCPSSAVENCIVSVSYRGNNLNGLWLGDAIYCPRHVLGKFSGDQWSDVLNLANNHEFEVVTQNGVTLNVVSRRLRGAVLILQTAVANADTPNYKFVKANCGDSFTIACSYGGTVVGLYPVTMRPNGTIRASFLAGACGSVGFNIEKGVVTFVYMHHLELPNALHTGTDLFGDFYGDYVDEEVAQRVPPDNLVTNNIVAWLYAAIISVKESSFSLPKWLESTTVSVEDYNKWAGDNGFTPFSTSTAITKLSAITGVDVCKLLRTIMVKSSQWGSDPILGQYNFEDELTPESVFNQVGGVRLQSSIVRRATSWFWSRCVLACFLFVLCAIVLFTALPLKYYVHAAVILLTAVLFISFTVKHVMAYMDTFLLPTLITVIIGACAEVPFIYNTLISQVVIFLSQWYDPVVFDTMVPWMLLPLVLYTAFKCVQGCYMDSFNTSLLMLYQSMKLGFVTYTSTNTLTAYTEGNWELFFELVHTTVLANVSSNSLIGLFVFKCAKWMLYYCNATYFNNYVLMAVMVNGLGWLCTCYFGLYWWVNKVFGLTLGKYNFKVSVDQYRYMCLHKIPAPKSVWDVVKTNILIQGIGGDRVLPIATVQSKLSDVKCTTVVLMQLLTKLNVEANSKMHAYLVELHNKILASDDVGECMDNLLGMLVTLFCIDSTIDLSEYCDDILKRSTVLQSVTQEFSYIPSYAEYERAKNLYEKVLADSKNGGVTQQELAAYRKAANIAKSVFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRAKLVSSLHALLFSMLKKIDSEKLNVLFDQASSGVVPLATVPIVCSNKLTLVIPDPETWVKCVEGMHVTYSTVVWNIDTVIDADGTELHPTSVGSGLTYCISSDNIAWPLKVNLTRNGHNKVDAALQNNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFGMKVGDKVEVVYLYFIKNTRSIVRGMVLGAISNVVVLQSKGHETEEVDAVGILSLCSFAVDPADTYCKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGGAGNLDGRCQFKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWIGHGCQCDAIRQQKPSVQSVAGASDFGKNYLNGYGVAVRLG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EU095850
EMBL· GenBank· DDBJ
ABW81436.1
EMBL· GenBank· DDBJ
Genomic RNA

Genome annotation databases

Similar Proteins

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