B3FHU4 · B3FHU4_CVTKE
- ProteinORF1a
- Gene1a
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids3951 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2 (By similarity).
Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses
Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses
Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.
Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities (By similarity).
Therefore plays an essential role in viral mRNAs cap methylation
Therefore plays an essential role in viral mRNAs cap methylation
Catalytic activity
Cofactor
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | host cell endoplasmic reticulum membrane | |
Cellular Component | host cell perinuclear region of cytoplasm | |
Cellular Component | membrane | |
Molecular Function | cysteine-type endopeptidase activity | |
Molecular Function | omega peptidase activity | |
Molecular Function | RNA binding | |
Molecular Function | transferase activity | |
Molecular Function | zinc ion binding | |
Biological Process | induction by virus of host autophagy | |
Biological Process | proteolysis | |
Biological Process | viral genome replication | |
Biological Process | viral protein processing | |
Biological Process | viral translational frameshifting |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Submitted names
Gene names
Organism names
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Gammacoronavirus > Igacovirus > Avian coronavirus
- Virus hosts
Accessions
- Primary accessionB3FHU4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Host endoplasmic reticulum membrane ; Multi-pass membrane protein
Membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 1747-1763 | Helical | ||||
Sequence: VLCKVVFATLLIVWFIY | ||||||
Transmembrane | 1844-1863 | Helical | ||||
Sequence: WLYLVFLILFVKPVAGFVII | ||||||
Transmembrane | 1870-1888 | Helical | ||||
Sequence: LVLNSTVLLTGVGFLDWFV | ||||||
Transmembrane | 1894-1912 | Helical | ||||
Sequence: HFNFMGAGFYFWLFYKIYI | ||||||
Transmembrane | 2281-2299 | Helical | ||||
Sequence: WLLIFYVLFTLCCLGYYHM | ||||||
Transmembrane | 2558-2579 | Helical | ||||
Sequence: IYMQLATMFLILVIVVLIFAMV | ||||||
Transmembrane | 2586-2606 | Helical | ||||
Sequence: FKAYATIVFTIMLVWAVNAFV | ||||||
Transmembrane | 2612-2630 | Helical | ||||
Sequence: YNSVVAAILLVLYCYASLV | ||||||
Transmembrane | 2642-2667 | Helical | ||||
Sequence: WLVFTFGLIVPIWLACCYLAFVLYVY | ||||||
Transmembrane | 3096-3116 | Helical | ||||
Sequence: WFWSRCVLACFLFVLCAIVLF | ||||||
Transmembrane | 3123-3142 | Helical | ||||
Sequence: YYVHAAVILLTAVLFISFTV | ||||||
Transmembrane | 3154-3183 | Helical | ||||
Sequence: LPTLITVIIGACAEVPFIYNTLISQVVIFL | ||||||
Transmembrane | 3190-3209 | Helical | ||||
Sequence: VVFDTMVPWMLLPLVLYTAF | ||||||
Transmembrane | 3263-3285 | Helical | ||||
Sequence: VLANVSSNSLIGLFVFKCAKWML | ||||||
Transmembrane | 3297-3320 | Helical | ||||
Sequence: VLMAVMVNGLGWLCTCYFGLYWWV |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Disulfide bond | 1802↔1808 | |||||
Sequence: CADDFTC |
Keywords
- PTM
Interaction
Subunit
Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure (By similarity).
Interacts with ORF6 protein
Interacts with ORF6 protein
Homodimer.
Interacts with host PHB and PHB2.
Interacts with papain-like protease and non-structural protein 6.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 675-780 | Ubiquitin-like | ||||
Sequence: KTVTFGGTTVKEIPPPDVVPIKVSIECCGEPWNTIFKKAYKEPIEVETDLTVEQLLSVIYEKMCDDLKLFPEAPEPPPFENVALVDKNGKDLDCIKSCHLIYRDYE | ||||||
Domain | 1005-1181 | Macro | ||||
Sequence: VKPATCEKPKFLEYKTCVGDLTVVIAKALDEFKEFCIVNAANEHMSHGGGVAKAIADFCGPDFVEYCEDYVKKHGPQQRLVTPSFVKGIQCVNNVVGPRHGDSNLREKLVAAYKKVLVDGVFNYVVPVLSSGIFGVDFKMSIDAMREAFDGCDIRVLLFSLSPEHIDYFNATCKQKT | ||||||
Domain | 1177-1229 | Ubiquitin-like | ||||
Sequence: CKQKTLYLTEDGVKYRSIVVKPGDSLIQFRQVFAKNKAVFTADDVEDKEILFV | ||||||
Domain | 1238-1499 | Peptidase C16 | ||||
Sequence: EYYHLDAQKYVIYLQTLAQKWNVQYRDNFIILEWRDGNCWVSSVAVLLQAAKIRFRGFLAEAWAKFLGGDPTEFVAWCYASCNAKVGDFSDTNWLLANLAEHFDADYTNALLKKRVSCNCGVKSYELRGLEACIQPVKAPNLLHFKTQYSNCSICGANSTDEVIEASLPYLLLFATDGPAAVDCDENAVGTVVFIGSTNSGHCYTQAIDKAFDNLAKDRKFGKKSPYITAMYMRFSLKSENPLPVVKQSKGKTKVVKEDVSN | ||||||
Domain | 1769-1833 | 3Ecto | ||||
Sequence: IFTGIRVLDFLVEGSLCGPYNDYGKDSFDVLRYCADDFTCRVCLHDRDSLHLYKHAYSVEQIYKA | ||||||
Region | 1911-2001 | Y1 | ||||
Sequence: YIQVYHILYCKDVTCEVCKRVARSNRQEVSVVVGGRKQIVHVYTNSGYKFCKKHNWYCRNCDDYGHQNTFMSPEVAGELSEKLKRHVKPTA | ||||||
Domain | 1911-2263 | CoV Nsp3 Y | ||||
Sequence: YIQVYHILYCKDVTCEVCKRVARSNRQEVSVVVGGRKQIVHVYTNSGYKFCKKHNWYCRNCDDYGHQNTFMSPEVAGELSEKLKRHVKPTAYAYHVVDEACLVDDFVNLKYKAAIPGKDSAPLAVKCFSITDFLKKAVFLKDALKCEQISNDGFIVCNTQSAHALEDAKNAAIYYAQYLCKPILLLDQVLYEQLVVEPVSKSVVDKVCSILSNIISVDTAALNYKAGTLRDALLSITKDEEAVDMAIFCHNHDVEYTSDGFTNVIPSYGIDTDKLTPRDRGFLINADASIANLRVKNAPPVVWKFSDLIKLSDSCLKYLISATVKSGVRFFITKSGAKQVISCHTQKLLVEKK | ||||||
Region | 1915-1928 | ZF1 | ||||
Sequence: YHILYCKDVTCEVC | ||||||
Region | 1961-1971 | ZF2 | ||||
Sequence: CKKHNWYCRNC | ||||||
Region | 2002-2263 | CoV-Y | ||||
Sequence: YAYHVVDEACLVDDFVNLKYKAAIPGKDSAPLAVKCFSITDFLKKAVFLKDALKCEQISNDGFIVCNTQSAHALEDAKNAAIYYAQYLCKPILLLDQVLYEQLVVEPVSKSVVDKVCSILSNIISVDTAALNYKAGTLRDALLSITKDEEAVDMAIFCHNHDVEYTSDGFTNVIPSYGIDTDKLTPRDRGFLINADASIANLRVKNAPPVVWKFSDLIKLSDSCLKYLISATVKSGVRFFITKSGAKQVISCHTQKLLVEKK | ||||||
Region | 2164-2263 | Y4 | ||||
Sequence: VEYTSDGFTNVIPSYGIDTDKLTPRDRGFLINADASIANLRVKNAPPVVWKFSDLIKLSDSCLKYLISATVKSGVRFFITKSGAKQVISCHTQKLLVEKK | ||||||
Domain | 2684-2779 | Nsp4C | ||||
Sequence: LYDGNEFVGNYDLAAKSTFVIRGSEFVKLTNEIGDKFEAYLSAYARLKYYSGTGSEQDYLQACRAWLAYALDQYRNSGVEIVYTPPRYSIGVSRLQ | ||||||
Domain | 2780-3086 | Peptidase C30 | ||||
Sequence: AGFKKLVCPSSAVENCIVSVSYRGNNLNGLWLGDAIYCPRHVLGKFSGDQWSDVLNLANNHEFEVVTQNGVTLNVVSRRLRGAVLILQTAVANADTPNYKFVKANCGDSFTIACSYGGTVVGLYPVTMRPNGTIRASFLAGACGSVGFNIEKGVVTFVYMHHLELPNALHTGTDLFGDFYGDYVDEEVAQRVPPDNLVTNNIVAWLYAAIISVKESSFSLPKWLESTTVSVEDYNKWAGDNGFTPFSTSTAITKLSAITGVDVCKLLRTIMVKSSQWGSDPILGQYNFEDELTPESVFNQVGGVRLQ | ||||||
Domain | 3380-3462 | RdRp Nsp7 cofactor | ||||
Sequence: SKLSDVKCTTVVLMQLLTKLNVEANSKMHAYLVELHNKILASDDVGECMDNLLGMLVTLFCIDSTIDLSEYCDDILKRSTVLQ | ||||||
Domain | 3463-3672 | RdRp Nsp8 cofactor | ||||
Sequence: SVTQEFSYIPSYAEYERAKNLYEKVLADSKNGGVTQQELAAYRKAANIAKSVFDRDLAVQKKLDSMAERAMTTMYKEARVTDRRAKLVSSLHALLFSMLKKIDSEKLNVLFDQASSGVVPLATVPIVCSNKLTLVIPDPETWVKCVEGMHVTYSTVVWNIDTVIDADGTELHPTSVGSGLTYCISSDNIAWPLKVNLTRNGHNKVDAALQ | ||||||
Domain | 3673-3783 | Nsp9 ssRNA-binding | ||||
Sequence: NNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFGMKVGDKVEVVYLYFIKNTRSIVRGMVLGAISNVVVLQ | ||||||
Domain | 3785-3926 | ExoN/MTase coactivator | ||||
Sequence: KGHETEEVDAVGILSLCSFAVDPADTYCKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGGAGNLDGRCQFKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWIGHGCQCDAIRQQKPS |
Sequence similarities
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length3,951
- Mass (Da)440,652
- Last updated2008-07-22 v1
- Checksum8051C3E28F720572
Keywords
- Coding sequence diversity
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
EU095850 EMBL· GenBank· DDBJ | ABW81436.1 EMBL· GenBank· DDBJ | Genomic RNA |