B3EWH0 · TXPR2_ALOMR
- ProteinPurotoxin-2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Enhances the high-affinity desensitization of human P2RX3 purinoceptors (PubMed:22842000).
At 50 nM, the toxin decreases the IC50 for ambient ATP from 2.67 nM to 0.77 nM in human P2RX3 (PubMed:22842000).
At 50 nM, the toxin decreases the IC50 for ambient ATP from 2.67 nM to 0.77 nM in human P2RX3 (PubMed:22842000).
Miscellaneous
Negative results: does not affect mouse P2RX2 or P2RX2/P2RX3 purinoceptors (PubMed:22842000).
The toxin (and its derivatives composed of N-terminal or C-terminal domains) are inactive against a number of bacterial strains (PubMed:27412961).
The toxin (and its derivatives composed of N-terminal or C-terminal domains) are inactive against a number of bacterial strains (PubMed:27412961).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | calcium channel inhibitor activity | |
Molecular Function | toxin activity |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended namePurotoxin-2
- Short namesPT2
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Chelicerata > Arachnida > Araneae > Araneomorphae > Entelegynae > Lycosoidea > Lycosidae > Alopecosa
Accessions
- Primary accessionB3EWH0
Subcellular Location
PTM/Processing
Features
Showing features for peptide, disulfide bond, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Peptide | PRO_0000419528 | 1-64 | Purotoxin-2 | |||
Sequence: AKACTPLLHDCSHDRHSCCRGDMFKYVCDCFYPEGEDKTEVCSCQQPKSHKIAEKIIDKAKTTL | ||||||
Disulfide bond | 4↔19 | |||||
Sequence: CTPLLHDCSHDRHSCC | ||||||
Disulfide bond | 11↔28 | |||||
Sequence: CSHDRHSCCRGDMFKYVC | ||||||
Disulfide bond | 18↔44 | |||||
Sequence: CCRGDMFKYVCDCFYPEGEDKTEVCSC | ||||||
Disulfide bond | 30↔42 | |||||
Sequence: CFYPEGEDKTEVC | ||||||
Modified residue | 64 | Leucine amide | ||||
Sequence: L |
Post-translational modification
Amidation at Leu-64 is not mandatory for activity on P2RX3.
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-44 | Knottin domain | ||||
Sequence: AKACTPLLHDCSHDRHSCCRGDMFKYVCDCFYPEGEDKTEVCSC | ||||||
Region | 45-64 | Linear cationic cytotoxin domain | ||||
Sequence: QQPKSHKIAEKIIDKAKTTL |
Domain
The toxin is composed of 2 domains: a highly rigid N-terminal inhibitor cystine knot (knottin) domain and a rather flexible C-terminal linear cationic cytotoxin domain that forms amphiphilic alpha-helices.
The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
Sequence similarities
Belongs to the neurotoxin 19 (CSTX) family. 05 (U4-Lctx) subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length64
- Mass (Da)7,264
- Last updated2012-10-03 v1
- ChecksumBB96626328CFCC59
Mass Spectrometry
Keywords
- Technical term