B3EWH0 · TXPR2_ALOMR

Function

function

Enhances the high-affinity desensitization of human P2RX3 purinoceptors (PubMed:22842000).
At 50 nM, the toxin decreases the IC50 for ambient ATP from 2.67 nM to 0.77 nM in human P2RX3 (PubMed:22842000).

Miscellaneous

Negative results: does not affect mouse P2RX2 or P2RX2/P2RX3 purinoceptors (PubMed:22842000).
The toxin (and its derivatives composed of N-terminal or C-terminal domains) are inactive against a number of bacterial strains (PubMed:27412961).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Molecular Functioncalcium channel inhibitor activity
Molecular Functiontoxin activity

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Purotoxin-2
  • Short names
    PT2
  • Alternative names
    • OMICRON-lycotoxin-Am1b
      (OMICRON-LCTX-Am1b
      )

Organism names

Accessions

  • Primary accession
    B3EWH0

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for peptide, disulfide bond, modified residue.

TypeIDPosition(s)Description
PeptidePRO_00004195281-64Purotoxin-2
Disulfide bond4↔19
Disulfide bond11↔28
Disulfide bond18↔44
Disulfide bond30↔42
Modified residue64Leucine amide

Post-translational modification

Amidation at Leu-64 is not mandatory for activity on P2RX3.

Keywords

Expression

Tissue specificity

Expressed by the venom gland.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-44Knottin domain
Region45-64Linear cationic cytotoxin domain

Domain

The toxin is composed of 2 domains: a highly rigid N-terminal inhibitor cystine knot (knottin) domain and a rather flexible C-terminal linear cationic cytotoxin domain that forms amphiphilic alpha-helices.
The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    64
  • Mass (Da)
    7,264
  • Last updated
    2012-10-03 v1
  • Checksum
    BB96626328CFCC59
AKACTPLLHDCSHDRHSCCRGDMFKYVCDCFYPEGEDKTEVCSCQQPKSHKIAEKIIDKAKTTL

Mass Spectrometry

Molecular mass is 7,255.5 Da. Determined by MALDI.

Keywords

Similar Proteins

Disclaimer

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