B3E3N9 · B3E3N9_TRIL1

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site95Proton acceptor
Binding site115[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site119[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site122[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site160-161S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site192S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site214-216S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site290S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site333S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Probable dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • Ordered locus names
      Glov_3610

Organism names

Accessions

  • Primary accession
    B3E3N9

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain101-323Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    351
  • Mass (Da)
    39,054
  • Last updated
    2008-07-22 v1
  • Checksum
    62C2F7741699E785
MPDSLRTDLKNLTLPALEQFLQGQGKERYRATQIFKWLYQHDVSSFDEMTNVSKALRAELVRTACISRLEPETVEVGSDGTRKYLFMLEDGNAVESVIIPDEDRNTLCISSQAGCAMQCAFCLTGTFSLTRNLTTAEIVNQILAVQRDVEVRNIVMMGMGEPLHNLDNVIPALQIMAEDNGLQLSSRRVTVSTCGLVPELERLGREVTVNLAVSLNATTDELRDRIMPVNKAYPIATLLAALKNYPLPGRRKITIEYVLLGGLNDTPEDAKRLVRLLSDIPCKINLIPFNPHEGADFRPPSRAALDAFHKYLLDRHFTVITRDSRGSDISAACGQLKGKLDRKPSQESPDV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001089
EMBL· GenBank· DDBJ
ACD97311.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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