B3DWU2 · DNLJ_METI4
- ProteinDNA ligase
- GeneligA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids689 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 58-62 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: DQEYD | ||||||
Binding site | 107-108 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: SL | ||||||
Binding site | 138 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 140 | N6-AMP-lysine intermediate | ||||
Sequence: K | ||||||
Binding site | 161 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 198 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 314 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 338 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 432 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 435 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 448 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 453 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | DNA binding | |
Molecular Function | DNA ligase (NAD+) activity | |
Molecular Function | metal ion binding | |
Biological Process | base-excision repair, DNA ligation | |
Biological Process | DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Verrucomicrobiota > Methylacidiphilae > Methylacidiphilales > Methylacidiphilaceae > Methylacidiphilum (ex Ratnadevi et al. 2023)
Accessions
- Primary accessionB3DWU2
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000380416 | 1-689 | DNA ligase | |||
Sequence: MIESYLAFIFLNKVNKKKAPSLDSLGYEEIKKRHDELVKLIRKYDYAYYVEAHPLVSDQEYDNLYHELETLEKLHPELITPDSPTQRIGETPLSGFSQVTHEIPMLSLENTYSKEELFAFLERIKRALPGKKISFTVEPKIDGVSISAVYKNGLFSLGATRGNGTVGDDITQNLKTIRSLPLRLETPDPPEYLEVRGEAYMSPKDFERLNAQREKEGKVLFANPRNATAGSLKQLDPRVVAERPLAVVFYGAGKLVGMKCKTQEEWLNFLKKIGLPIPIVFWVCSNENEVYEAIGKLNECRNQLPYPTDGAAVKVNEWEYYSLLGYTAKAPRWAFAYKYGAERAKTRLNNVIFQVGRSGTITPVAEMDPVFLSGTTVSRATLHNFDQVKRLDVKIGDVVYLEKAGEVIPEVVGVDLNQRRGTEKEIVPPEFCPSCGEKLSWEGIFLRCENENCPAQLKERILHFAQRNAMDIQGLGESLVDQLVDKGIVKDVADIYDLDEETLVNLDRMGKKSAQNLLKAIEESKKKDLSRLIFGLGIPHIGQKASEDLARYFGTMDKLSHATEEELLNLPFIGEIMARSIVNYFRKEANRRRLEKLRKKGLNFVSTLSQASSGTLSGKTFVITGTLSEPRESIAQKIISKGGRVSNSLSRKTSYLVVGSDPGSKLQEAKKLGIPLINEQELLEMLHGG |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 611-689 | BRCT | ||||
Sequence: ASSGTLSGKTFVITGTLSEPRESIAQKIISKGGRVSNSLSRKTSYLVVGSDPGSKLQEAKKLGIPLINEQELLEMLHGG |
Sequence similarities
Belongs to the NAD-dependent DNA ligase family. LigA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length689
- Mass (Da)77,418
- Last updated2008-07-22 v1
- ChecksumA067A22CB46CB6DE
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000975 EMBL· GenBank· DDBJ | ACD83755.1 EMBL· GenBank· DDBJ | Genomic DNA |