B3DV19 · IXTPA_METI4
- ProteindITP/XTP pyrophosphatase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids202 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.
Catalytic activity
- H2O + XTP = diphosphate + H+ + XMP
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 10-15 | substrate | ||||
Sequence: TSNRHK | ||||||
Active site | 70 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 70 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 71 | substrate | ||||
Sequence: S | ||||||
Binding site | 153-156 | substrate | ||||
Sequence: FGYD | ||||||
Binding site | 176 | substrate | ||||
Sequence: K | ||||||
Binding site | 181-182 | substrate | ||||
Sequence: HR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | dITP diphosphatase activity | |
Molecular Function | ITP diphosphatase activity | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotide binding | |
Molecular Function | ribonucleoside triphosphate phosphatase activity | |
Molecular Function | XTP diphosphatase activity | |
Biological Process | nucleotide metabolic process | |
Biological Process | purine nucleoside triphosphate catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namedITP/XTP pyrophosphatase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Verrucomicrobiota > Methylacidiphilae > Methylacidiphilales > Methylacidiphilaceae > Methylacidiphilum (ex Ratnadevi et al. 2023)
Accessions
- Primary accessionB3DV19
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000145494 | 1-202 | dITP/XTP pyrophosphatase | |||
Sequence: MIMQKILLATSNRHKYLEFSRLLYPLTLEALPEDLKRLLPNETAKSYRDNAKLKGMALSEIYEGFVLADDSGLEVFSLHGEPGIFSSRYAGEGSSAQENIDKLLKNLQSKNITDRRARFVCALVLVKKKKILFETTAFCYGIIADRQKGGGGFGYDPIFIPEGYSLTMAELDEKQKDLVSHRGKACQELKAFFLENRMEGHS |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length202
- Mass (Da)22,778
- Last updated2008-07-22 v1
- ChecksumB627AD1D7209F431
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000975 EMBL· GenBank· DDBJ | ACD83172.1 EMBL· GenBank· DDBJ | Genomic DNA |