B3DLA6 · DICER_XENTR

Function

function

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes (By similarity).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 magnesium or manganese ions per subunit.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site54-61ATP (UniProtKB | ChEBI)
Binding site1293Mg2+ 1 (UniProtKB | ChEBI)
Binding site1371Mg2+ 1 (UniProtKB | ChEBI)
Binding site1374Mg2+ 1 (UniProtKB | ChEBI)
Binding site1676Mg2+ 2 (UniProtKB | ChEBI)
Site1777Important for activity
Binding site1781Mg2+ 2 (UniProtKB | ChEBI)
Binding site1784Mg2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular ComponentRISC complex
Cellular ComponentRISC-loading complex
Molecular FunctionATP binding
Molecular Functionhelicase activity
Molecular Functionmetal ion binding
Molecular Functionribonuclease III activity
Molecular FunctionRNA binding
Biological Processglobal gene silencing by mRNA cleavage
Biological Processpre-miRNA processing
Biological ProcesssiRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Gene names

    • Name
      dicer1

Organism names

Accessions

  • Primary accession
    B3DLA6
  • Secondary accessions
    • C0IN01

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003739861-1893Endoribonuclease Dicer

Keywords

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of dicer1, ago2 and tarbp2; dicer1 and tarbp2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto ago2. Note that the trimeric RLC/miRLC is also referred to as RISC (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, motif, region, compositional bias.

TypeIDPosition(s)Description
Domain41-217Helicase ATP-binding
Motif165-168DECH box
Region400-424Disordered
Compositional bias402-418Acidic residues
Domain424-593Helicase C-terminal
Domain621-713Dicer dsRNA-binding fold
Region718-737Disordered
Domain886-1036PAZ
Domain1249-1380RNase III 1
Domain1637-1795RNase III 2
Domain1820-1885DRBM

Sequence similarities

Belongs to the helicase family. Dicer subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

B3DLA6-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    1,893
  • Mass (Da)
    215,398
  • Last updated
    2009-05-26 v2
  • Checksum
    EAA01EFBC49FF8F3
MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNSGSGKTFIAVLLSKELSYQIRGDFSKNTKRTVFLVNSEKQVSQQVSAVRTHTDLKVGEYSDQEKTQCWAKERWYLEFETHQVLVMTCHIFLNVLKSGNVSLSNINLLVFDECHLAIQDHPYREIMKICESCQPCPRILGLTASILNGKCDPRDLEEKIQKLEEILRSNAETATDLVVLDRYASQPCEIVLDCGPYIDKSGLYQRLLNELDEALNFLIDCNISTHSKERDSTLISKQILSDCQTVLLVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTILRKIHALCEEHFSPASLDMKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDEDEEIEEKEKTETSFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPSEYRSYVQSKGRARAPISNYIMLADSDKIKAFEEDLKTYKAIEKILRNKCSKSIDCGNTESEPIVDDDEIFPPYVLRQDDGSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTREFPDGLYRSTLYLPINSPLRAPIVGPPMNCGRLADRAVALICCKKLHEIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRNSYPKPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLNLEQLELITRLHQYIFSHILRLEKPALEFKPTVADCAYCVLPLNVVNDSGTLDIDFKFVEDIEKSEARTGIPNTQYSAESPFIFKLEDYQDAVIIPRQVIYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPVPASLWRKAVCLPSILYRLHCLLTAEELRAQTAIDAGVGVKSLPDDFRYPNLDFGWKRSIDSKTFISNQSSSSVESESDCRLNKTTAPDSAASSAANSVIYMQINDQMSVNCTPPCQKSLSHLQTVCFSDDYKAINGISCNGLTNGDWEAESAACFQKDERITCKQEIPEKSTSFHVQNLPKENQPILKECTLSNSDGNVSKPTSDECPSTCTSDMHYDSGLSNRHSSKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGSPSRMVVSIFDPPVNWLPPGYIVNQDKNSDKWESNETSGEDVMVNGKIDEDFDDEEDEDLMWRNPKEETDFDDDFLEYDQEHIKFIDSMLMGSGAFVKKIPLSSFAPPDQNYEWRAPKKPPLESSQFPCDFDDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGADAGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPEVRKLVTNTNVISASSSYQNSTRDNCTLTARTNTDLSSCKGIDYGYLKIPPRCMFEHPDAEKTLDHLISGFENFEKKINYPFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAISPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWHVYYPMMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQSQVPNS

B3DLA6-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8J1IMX6A0A8J1IMX6_XENTRdicer11890

Features

Showing features for compositional bias, alternative sequence.

TypeIDPosition(s)Description
Compositional bias402-418Acidic residues
Alternative sequenceVSP_037298804-811in isoform 2
Alternative sequenceVSP_037299812-1893in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EU338242
EMBL· GenBank· DDBJ
ACA52289.1
EMBL· GenBank· DDBJ
mRNA
BC167373
EMBL· GenBank· DDBJ
AAI67373.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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