B2WLB5 · B2WLB5_PYRTR
- ProteinATP-dependent DNA helicase II subunit 1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids655 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 21 | Schiff-base intermediate with DNA; for 5'-deoxyribose-5-phosphate lyase activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome, subtelomeric region | |
Cellular Component | chromosome, telomeric repeat region | |
Cellular Component | Ku70:Ku80 complex | |
Cellular Component | pericentric heterochromatin | |
Cellular Component | site of double-strand break | |
Molecular Function | ATP binding | |
Molecular Function | damaged DNA binding | |
Molecular Function | DNA helicase activity | |
Molecular Function | double-stranded DNA binding | |
Molecular Function | hydrolase activity | |
Molecular Function | telomeric DNA binding | |
Biological Process | DNA recombination | |
Biological Process | double-strand break repair via nonhomologous end joining | |
Biological Process | stalled replication fork localization to nuclear periphery | |
Biological Process | telomere maintenance |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent DNA helicase II subunit 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Dothideomycetes > Pleosporomycetidae > Pleosporales > Pleosporineae > Pleosporaceae > Pyrenophora
Accessions
- Primary accessionB2WLB5
Proteomes
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-21 | Disordered | |||
Region | 34-54 | Disordered | |||
Region | 553-616 | Disordered | |||
Compositional bias | 560-597 | Basic and acidic residues | |||
Domain | 616-650 | SAP | |||
Sequence similarities
Belongs to the ku70 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length655
- Mass (Da)73,315
- Last updated2008-07-01 v1
- MD5 ChecksumED1FC73D87D05556A4193ED14C779522
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 560-597 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DS231629 EMBL· GenBank· DDBJ | EDU43825.1 EMBL· GenBank· DDBJ | Genomic DNA |