B2VL10 · PQQE_ERWT9
- ProteinPqqA peptide cyclase
- GenepqqE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids382 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the cross-linking of a glutamate residue and a tyrosine residue in the PqqA protein as part of the biosynthesis of pyrroloquinoline quinone (PQQ).
Catalytic activity
- [PQQ precursor protein] + S-adenosyl-L-methionine = 5'-deoxyadenosine + E-Y cross-linked-[PQQ precursor protein] + H+ + L-methionine
Cofactor
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway
Cofactor biosynthesis; pyrroloquinoline quinone biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | cyclase activity | |
Molecular Function | iron ion binding | |
Molecular Function | oxidoreductase activity | |
Molecular Function | S-adenosyl-L-methionine binding | |
Biological Process | pyrroloquinoline quinone biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePqqA peptide cyclase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Erwiniaceae > Erwinia
Accessions
- Primary accessionB2VL10
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000131275 | 1-382 | PqqA peptide cyclase | |||
Sequence: MNPSESNVKPPLWLLAELTYRCPLQCPYCSNPLDFAQQEKELSTAQWIEVFKQARAMGAVQIGFSGGEPLVRKDLPELIRSARELGFYTNLITSGIGLTQKKIDAFAEAGLDHIQISFQASDETLNAALAGSQKAFQQKLEMARAVKAHGYPMVLNFVLHRHNIDQLDRIIELCIELEADDVELATCQFYGWAQLNREGLLPTRDQLVRAEAVVHRYREKMAASGNLANLLFVTPDYYEERPKGCMGGWGAIFLSVTPEGMALPCHSARQLPIRFPSVLEHSLQDIWFNSFGFNRYRGFDWMPEPCRSCDEKEKDFGGCRCQAFMLTGNADNADPVCSKSEHHGTILAAREQANCTNIQVNQLRFRNRANSERVNSQLIFKG |
Interaction
Subunit
Interacts with PqqD. The interaction is necessary for activity of PqqE.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 8-223 | Radical SAM core | ||||
Sequence: VKPPLWLLAELTYRCPLQCPYCSNPLDFAQQEKELSTAQWIEVFKQARAMGAVQIGFSGGEPLVRKDLPELIRSARELGFYTNLITSGIGLTQKKIDAFAEAGLDHIQISFQASDETLNAALAGSQKAFQQKLEMARAVKAHGYPMVLNFVLHRHNIDQLDRIIELCIELEADDVELATCQFYGWAQLNREGLLPTRDQLVRAEAVVHRYREKMAA |
Sequence similarities
Belongs to the radical SAM superfamily. PqqE family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length382
- Mass (Da)43,040
- Last updated2008-07-01 v1
- ChecksumEAE66910F563AAE6
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CU468135 EMBL· GenBank· DDBJ | CAO97932.1 EMBL· GenBank· DDBJ | Genomic DNA |