B2T6I7 · SYI_PARPJ

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site581L-isoleucyl-5'-AMP (UniProtKB | ChEBI)
Binding site625ATP (UniProtKB | ChEBI)
Binding site908Zn2+ (UniProtKB | ChEBI)
Binding site911Zn2+ (UniProtKB | ChEBI)
Binding site928Zn2+ (UniProtKB | ChEBI)
Binding site931Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • Ordered locus names
      Bphyt_3159

Organism names

Accessions

  • Primary accession
    B2T6I7

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10001891401-945Isoleucine--tRNA ligase

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif66-76'HIGH' region
Motif622-626'KMSKS' region

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    945
  • Mass (Da)
    105,985
  • Last updated
    2008-07-01 v1
  • Checksum
    B698B672B54B10FA
MSNKKADSKPQSRYPVNLLDTPFPMRGDLPKREPQWVKEWQERKVYETIRAASKGRKKFILHDGPPYANGDIHLGHAVNKILKDMIVKARNLAGFDAVYVPGWDCHGMPIEIQIEKQFGKSLPAAEVMQKARAYATEQIEKQKVGFRRLGVLGDWDNPYKTMNFTNEAGEIRALAKIMEKGYVFRGLKPVNWCFDCGSALAEAEVEYKDKTDPTIDVLFSFAEPEKTAQAFGLSALPREEGGIVIWTTTPWTIPANQALNLHPEIVYALVDTPRGLLILAEERVEACLKLYGLEGTIVATAPGAKLVNVRFNHPLASAHPGYKRTAPVFLGDYVTTETGTGIVHSSPAYGVEDFVSCKAHGMADSDIINPVMGDGRYIESLALFGGLSIWAANPQIVEALQGAGSLMRTEKYTHSYMHCWRHKTPIIYRATSQWFAGMDVKPNDTDKTLRETALEGIENTAFYPAWGKQRLFSMIANRPDWTLSRQRQWGVPMAFFVHKETGELHPRTLELLEEVAQRVEKAGIEAWQTLDPRELLGDDANMYEKNRDTLDVWFDSGTTHWHVLRGSHKDELQFPADLYLEGSDQHRGWFHSSLLTASMLDGRPPYNALLTHGFTVDGEGRKMSKSLGNGIDPHEVANRLGAEIIRLWIASTDYSGELAISEEILKRVTEGYRRIRNTLRFLLANLSDFDFAQHARPVEDWLEIDRYAVALSTNLQNDILSHYDKYEFHPVVAKLQTFCSEDLGGFYLDVLKDRLYTTAADSVARRSAQTALYHIAHGLLRLMAPFLSFTAEEAWKIFQPNSETIFTETYHAFPAVPDAGALLDKWTLLRAARSDVTKALEEARVANLIGSSLQAEVEIRASGARYDALTSLGDDLKFVLITSAATVVKVDSEAEEAVEVIASKYLKCERCWHYRADVGANAEHPTLCDRCFSNLFGNGEIRSAA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001052
EMBL· GenBank· DDBJ
ACD17551.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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