B2S544 · NUOB_BRUA1
- ProteinNADH-quinone oxidoreductase subunit B
- GenenuoB
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids193 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).
Catalytic activity
- a quinone + NADH + 5 H+(in) = a quinol + NAD+ + 4 H+(out)
CHEBI:132124 + CHEBI:57945 + 5 H+ (in)CHEBI:15378= CHEBI:24646 + CHEBI:57540 + 4 H+ (out)CHEBI:15378
Cofactor
Note: Binds 1 [4Fe-4S] cluster.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 72 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 73 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 137 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 167 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | iron ion binding | |
Molecular Function | NADH dehydrogenase (ubiquinone) activity | |
Molecular Function | NADH:ubiquinone reductase (non-electrogenic) activity | |
Molecular Function | quinone binding | |
Biological Process | aerobic respiration | |
Biological Process | electron transport coupled proton transport |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADH-quinone oxidoreductase subunit B
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Brucellaceae > Brucella/Ochrobactrum group > Brucella
Accessions
- Primary accessionB2S544
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000358357 | 1-193 | NADH-quinone oxidoreductase subunit B | ||
Keywords
- PTM
Interaction
Subunit
NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.
Structure
Sequence
- Sequence statusComplete
- Length193
- Mass (Da)21,036
- Last updated2008-07-01 v1
- ChecksumF85E7FEDA4E09A25
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000887 EMBL· GenBank· DDBJ | ACD72291.1 EMBL· GenBank· DDBJ | Genomic DNA |