B2RHG3 · MFA3_PORG3

Function

function

Tip subunit of the minor fimbriae. These filamentous pili are attached to the cell surface; they mediate biofilm formation, adhesion onto host cells and onto other bacteria that are part of the oral microbiome (PubMed:24118823, PubMed:26001707).
They play an important role in invasion of periodontal tissues and are recognized as major virulence factors. Fimbrium subunits from different strains have highly divergent sequences, and this correlates with pathogenicity (Probable)

Miscellaneous

The name (minor fimbrium subunit) does not indicate the abundance of the protein, but is derived from the greater length of the major fimbriae. In strain ATCC 33277 and strain ATCC BAA-1703 / FDC 381, major fimbriae are 300 - 1600 nM in length and about 5 nm in diameter. In contrast, minor fimbriae are only about 80 - 120 nm long. This length difference is observed only in a small number of strains, including strain ATCC 33277 and strain ATCC BAA-1703 / FDC 381, and is due to a loss of function mutation in FimB, a protein that restricts fimbrial length in other strains.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell outer membrane
Cellular Componentpilus
Cellular Componentpilus tip

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Minor fimbrium tip subunit Mfa3

Gene names

    • Name
      mfa3
    • Ordered locus names
      PGN_0289

Organism names

Accessions

  • Primary accession
    B2RHG3

Proteomes

Subcellular Location

Fimbrium
Cell outer membrane
Note: Targeted to the outer membrane as a palmitoylated precursor. The lipid modification is no longer present after proteolytic processing to the mature form (PubMed:26437277).
Detected at the tip of the fimbriae (PubMed:24118823).

Keywords

Phenotypes & Variants

Disruption phenotype

Mildly increased autoaggregation.

PTM/Processing

Features

Showing features for signal, lipidation, propeptide, chain.

Type
IDPosition(s)Description
Signal1-20
Lipidation21N-palmitoyl cysteine
Lipidation21S-diacylglycerol cysteine
PropeptidePRO_000043679521-43
ChainPRO_500278164544-446Minor fimbrium tip subunit Mfa3

Keywords

Interaction

Subunit

Component of the fimbrium tip (PubMed:24118823).
Minor fimbriae are composed of a structural subunit, most often Mfa1, and the accessory subunits Mfa3, Mfa4 and Mfa5 (PubMed:19589838, PubMed:24118823, PubMed:26001707).
Fimbrium assembly occurs by linear, head-to-tail oligomerization of fimbrial subunits. This is mediated via insertion of a C-terminal beta-strand from one subunit into a groove in the N-terminal domain of the following subunit (Probable). Mfa3 is required for Mfa4 and Mfa5 insertion into the fimbrium (PubMed:24118823).

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    446
  • Mass (Da)
    50,047
  • Last updated
    2008-07-01 v1
  • Checksum
    A154AFE8FDC7972C
MMQLKKRYFALILLLFLWSGCDRGVDPQPDPLQPDVYLLVNARAAHTNGEESINMDAEDFEDRVHSLAMLVFDSNTGEKVAEHFSSSIGSGTSTYVFTVKLKPGQRDFFFVANIPNMQTAMASIVNKSDMNHFMQVFRDLDPIHYHNATNNNGFPMSRMYSNQTVTIGGTITQPLPFKPDGENNVKLQRVVAKLDVNIVEGVENLQKIELCNANVHYRLVPNQSEPIQFYGPVELRRVGATNQWLGYMPEAIVESTKWWGNTGNAENKPINFFRLTTRGGLVYDVPIITHEGAIPGGQYLPFAKGLLADKPSYTVYRNRHYIYRIKTLPDKIEVKYSICDWNIVTNDTYMGYGYNVGVDEQGNVTITNTMQNCDPHVVRLVAKNGAYFGSQPTDTSVEFTELANGASQTFKVNKDAVAVGSAYLEVYYNPDLNATGVVPDKVFIKK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP009380
EMBL· GenBank· DDBJ
BAG32808.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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