B2RAR3 · B2RAR3_HUMAN
- ProteinQueuine tRNA-ribosyltransferase catalytic subunit 1
- GeneQTRT1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids403 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.
Catalytic activity
- guanosine34 in tRNA + queuine = guanine + queuosine34 in tRNA
Cofactor
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 105 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 105-109 | substrate | ||||
Sequence: DSGGF | ||||||
Binding site | 159 | substrate | ||||
Sequence: D | ||||||
Binding site | 202 | substrate | ||||
Sequence: Q | ||||||
Binding site | 229 | substrate | ||||
Sequence: G | ||||||
Active site | 279 | Nucleophile | ||||
Sequence: D | ||||||
Binding site | 317 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 319 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 322 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 348 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | mitochondrial outer membrane | |
Molecular Function | metal ion binding | |
Molecular Function | tRNA-guanosine(34) queuine transglycosylase activity | |
Biological Process | tRNA-guanine transglycosylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameQueuine tRNA-ribosyltransferase catalytic subunit 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionB2RAR3
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion outer membrane ; Peripheral membrane protein
Note: Weakly associates with mitochondria, possibly via QTRT2.
Keywords
- Cellular component
PTM/Processing
Proteomic databases
Interaction
Subunit
Heterodimer of a catalytic subunit QTRT1 and an accessory subunit QTRT2.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 27-380 | tRNA-guanine15 transglycosylase-like | ||||
Sequence: RARAGELWLPHGTVATPVFMPVGTQATMKGITTEQLDALGCRICLGNTYHLGLRPGPELIQKANGLHGFMNWPHNLLTDSGGFQMVSLVSLSEVTEEGVRFRSPYDGNETLLSPEKSVQIQNALGSDIIMQLDDVVSSTVTGPRVEEAMYRSIRWLDRCIAAHQRPDKQNLFAIIQGGLDADLRATCLEEMTKRDVPGFAIGGLSGGESKSQFWRMVALSTSRLPKDKPRYLMGVGYATDLVVCVALGCDMFDCVFPTRTARFGSALVPTGNLQLRKKVFEKDFGPIDPECTCPTCQKHSRAFLHALLHSDNTAALHHLTVHNIAYQLQLMSAVRTSIVEKRFPGFVRDFMGAM | ||||||
Region | 260-266 | RNA binding | ||||
Sequence: GVGYATD | ||||||
Region | 284-288 | RNA binding; important for wobble base 34 recognition | ||||
Sequence: TRTAR |
Sequence similarities
Belongs to the queuine tRNA-ribosyltransferase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length403
- Mass (Da)43,990
- Last updated2008-07-01 v1
- Checksum1CCDDC17BF47A106