B2JGM6 · B2JGM6_PARP8
- ProteinPhosphoribosylformylglycinamidine synthase
- GenepurL
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1359 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
Catalytic activity
- ATP + H2O + L-glutamine + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N1-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H+ + L-glutamate + phosphate
Pathway
Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 325-336 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GAATGAGGEIRD | ||||||
Binding site | 714 | ATP (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 715 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 754 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 758 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 924 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 926 | ATP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Active site | 1199 | Nucleophile | ||||
Sequence: C | ||||||
Active site | 1320 | |||||
Sequence: H | ||||||
Active site | 1322 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoribosylformylglycinamidine synthase activity | |
Biological Process | 'de novo' IMP biosynthetic process | |
Biological Process | glutamine metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePhosphoribosylformylglycinamidine synthase
- EC number
- Short namesFGAM synthase ; FGAMS
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Paraburkholderia
Accessions
- Primary accessionB2JGM6
Proteomes
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 38-162 | Phosphoribosylformylglycinamidine synthase N-terminal | ||||
Sequence: QYLHFVNSQTPLSSEDSAKIEALMHYGDPFDAGKDKGAAETFLVVPRFGTVSPWASKATDIAHHCGLAHVRRIERGVEYTVVLKGGLLGGKKALSEAARAAVVAALHDRMTESVAPSRDHAMHLF | ||||||
Domain | 183-232 | Phosphoribosylformylglycinamidine synthase linker | ||||
Sequence: LEKANSELGLALADDEIDYLADAFTKLGRNPTDVELMMFAQANSEHCRHK | ||||||
Domain | 464-621 | PurM-like C-terminal | ||||
Sequence: AGSLLIQIGGPGMRIGMGGGAASSMATGTNTAELDFDSVQRGNPEIERRAQEVINSCWQLGEGNPILSIHDVGAGGLSNAFPELVDGADKGALFELRKIQLEESGLSPREIWSNEAQERYVLAIPPTRLEEFAAICERERCPFAVVGAATAERQLKLI | ||||||
Domain | 876-1021 | PurM-like C-terminal | ||||
Sequence: QRLGGSILAQVTQQVGDTVPDVDDAEDLKRFFAAIQALNADGKLLAYHDRSDGGLWTTVCEMAFAGHVGVSLNVDMLTLDPDHEFDYGDAKDWTKQTSGRREDRTIRALFNEELGAVVQVRAADRDAVLAALREHGLSACSHVIGK |
Sequence similarities
In the N-terminal section; belongs to the FGAMS family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,359
- Mass (Da)147,028
- Last updated2008-06-10 v1
- Checksum327E02AFEB2EEF2E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001043 EMBL· GenBank· DDBJ | ACC70211.1 EMBL· GenBank· DDBJ | Genomic DNA |