B2IZD3 · BDLP_NOSP7

Function

function

Dynamin-related GTPase probably involved in membrane remodeling. Lipid and nucleotide-binding are thought to induce a large intramolecular rearrangement, leading to assembly on lipid bilayers and possible membrane curving. In the presence of the non-hydrolyzable GTP analog GMP-PNP self-assembles on a lipid bilayer; this does not stimulate subsequent GTPase activity. Does not bind lipids in the presence of GDP; perhaps GTP hydrolysis disrupts membrane-binding.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
68.6 μMGTP
kcat is 0.53 min-1.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site79-84GTP (UniProtKB | ChEBI)
Binding site235-241GTP (UniProtKB | ChEBI)
Binding site292-293GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionidentical protein binding
Molecular Functionlipid binding
Biological Processmitochondrial fusion

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bacterial dynamin-like protein
  • EC number
  • Short names
    BDLP

Gene names

    • Ordered locus names
      Npun_R6513

Organism names

Accessions

  • Primary accession
    B2IZD3

Proteomes

Subcellular Location

Cell inner membrane
; Peripheral membrane protein
Note: Probably inserts into the outer leaflet of the membrane only (Probable). Forms foci localized in the cell periphery, and occasionally in the cell interior.

Features

Showing features for topological domain, intramembrane.

TypeIDPosition(s)Description
Topological domain1-521Cytoplasmic
Intramembrane522-574
Topological domain575-693Cytoplasmic

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis8215-fold reduction of GTP hydrolysis.
Mutagenesis576-577No lipid-binding.
Mutagenesis583No lipid-binding.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004255731-693Bacterial dynamin-like protein

Expression

Developmental stage

Detected in vegetatively growing cells (at protein level).

Interaction

Subunit

Homodimer. Self-assembles in the presence of GMP-PNP and liposomes, and probably also in the presence of GTP.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY B2IZD3Npun_R6513 B2IZD32EBI-15613218, EBI-15613218

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, coiled coil.

TypeIDPosition(s)Description
Domain66-313Dynamin-type G
Region76-83G1 motif
Region102-103G2 motif
Region180-183G3 motif
Region238-241G4 motif
Region268G5 motif
Region311-571Middle domain
Coiled coil347-378
Region572-606Paddle domain
Region607-693GED
Coiled coil661-688

Domain

The GTPase domain dimerizes and forms the BDLP tube surface, the middle and GED domains are elongated and involved in self-assembly, while the paddle region inserts into the outer leaflet of the membrane, possibly promoting membrane curvature.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    693
  • Mass (Da)
    78,348
  • Last updated
    2008-06-10 v1
  • Checksum
    DC1483C71AF1D80D
MVNQVATDRFIQDLERVAQVRSEMSVCLNKLAETINKAELAGDSSSGKLSLERDIEDITIASKNLQQGVFRLLVLGDMKRGKSTFLNALIGENLLPSDVNPCTAVLTVLRYGPEKKVTIHFNDGKSPQQLDFQNFKYKYTIDPAEAKKLEQEKKQAFPDVDYAVVEYPLTLLQKGIEIVDSPGLNDTEARNELSLGYVNNCHAILFVMRASQPCTLGERRYLENYIKGRGLTVFFLVNAWDQVRESLIDPDDVEELQASENRLRQVFNANLAEYCTVEGQNIYDERVFELSSIQALRRRLKNPQADLDGTGFPKFMDSLNTFLTRERAIAELRQVRTLARLACNHTREAVARRIPLLEQDVNELKKRIDSVEPEFNKLTGIRDEFQKEIINTRDTQARTISESFRSYVLNLGNTFENDFLRYQPELNLFDFLSSGKREAFNAALQKAFEQYITDKSAAWTLTAEKDINAAFKELSRSASQYGASYNQITDQITEKLTGKDVKVHTTTTAEEDNSPGWAKWAMGLLSLSKGNLAGFALAGAGFDWKNILLNYFTVIGIGGIITAVTGILLGPIGFALLGLGVGFLQADQARRELVKTAKKELVKHLPQVAHEQSQVVYNAVKECFDSYEREVSKRINDDIVSRKSELDNLVKQKQTREINRESEFNRLKNLQEDVIAQLQKIEAAYSNLLAYYS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001037
EMBL· GenBank· DDBJ
ACC84775.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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