B2HLY1 · NB2_MYCMM
- ProteinPeroxynitrite isomerase 2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids161 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Heme-binding protein able to scavenge peroxynitrite and to protect free L-tyrosine against peroxynitrite-mediated nitration, by acting as a peroxynitrite isomerase that converts peroxynitrite to nitrate. Therefore, this protein likely plays a role in peroxynitrite sensing and in the detoxification of reactive nitrogen and oxygen species (RNS and ROS, respectively). Is able to bind nitric oxide (NO) in vitro, but may act as a sensor of peroxynitrite levels in vivo.
Catalytic activity
- peroxynitrite = nitrateThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 1 heme b group per subunit, that coordinates a highly solvent-exposed Fe(III) atom.
Pathway
Nitrogen metabolism.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | heme binding | |
Molecular Function | metal ion binding | |
Molecular Function | peroxynitrite isomerase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePeroxynitrite isomerase 2
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium ulcerans group
Accessions
- Primary accessionB2HLY1
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000356923 | 1-161 | Peroxynitrite isomerase 2 | |||
Sequence: MPADLHPDLDALAPLLGTWAGQGSGEYPTIEPFEYLEEVVFSHVGKPFLVYAQKTRAVADGAPLHAETGYLRVPKPGQVELVLAHPSGITEIEVGTYSASGGVIEMEMVTTAIGMTPTAKEVTALSRSFRMVGDELSYRLRMGAVGLPLQHHLGARLRRKS |
Interaction
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 17-23 | GXWXGXG | ||||
Sequence: GTWAGQG |
Domain
Forms a 10-stranded antiparallel beta-barrel structure able to accommodate a hydrophobic ligand in its interior. In fact, this fold hosts the heme group, which is located in a wide surface cleft.
Sequence similarities
Belongs to the nitrobindin family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length161
- Mass (Da)17,354
- Last updated2008-06-10 v1
- ChecksumEB9998242E071B94
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000854 EMBL· GenBank· DDBJ | ACC40445.1 EMBL· GenBank· DDBJ | Genomic DNA |