B2HIL7 · MSL7_MYCMM
- ProteinPhenolphthiocerol synthesis polyketide synthase type I Pks15/1
- Genepks15/1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids2104 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the elongation by iterative transfer of p-hydroxybenzoyl group from FadD22 (pHBA-S-FAdD22) to form p-hydroxyphenylalkanoate (pHPA) intermediates during phenolphthiocerol (PPOL) biosynthesis. PPOL is an important intermediate in the biosynthesis of phenolic glycolipid (mycosid B).
Catalytic activity
- a fatty acyl-[ACP] + malonyl-[ACP] + H+ = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2
Cofactor
Note: Binds 1 phosphopantetheine covalently.
Pathway
Lipid metabolism; fatty acid biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 211 | For beta-ketoacyl synthase activity | |||
Active site | 346 | For beta-ketoacyl synthase activity | |||
Active site | 386 | For beta-ketoacyl synthase activity | |||
Active site | 662 | For acyltransferase activity | |||
Active site | 967 | Proton acceptor; for dehydratase activity | |||
Active site | 1128 | Proton donor; for dehydratase activity | |||
Binding site | 1530-1547 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 1719-1734 | NADP+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Cellular Component | polyketide synthase complex | |
Molecular Function | 3-oxoacyl-[acyl-carrier-protein] synthase activity | |
Molecular Function | fatty acid synthase activity | |
Molecular Function | oxidoreductase activity | |
Molecular Function | phosphopantetheine binding | |
Biological Process | Actinobacterium-type cell wall biogenesis | |
Biological Process | DIM/DIP cell wall layer assembly | |
Biological Process | fatty acid biosynthetic process | |
Biological Process | lipid biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhenolphthiocerol synthesis polyketide synthase type I Pks15/1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium ulcerans group
Accessions
- Primary accessionB2HIL7
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 211 | The pHBA starter unit is not loaded onto Pks15/1 and thus the pHPA intermediate is not produced. | |||
Mutagenesis | 2039 | The pHBA starter unit is loaded onto Pks15/1, but the pHPA intermediate is not produced. | |||
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000406361 | 1-2104 | Phenolphthiocerol synthesis polyketide synthase type I Pks15/1 | ||
Modified residue | 2039 | O-(pantetheine 4'-phosphoryl)serine | |||
Keywords
- PTM
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 41-464 | Ketosynthase family 3 (KS3) | |||
Region | 571-887 | Acyltransferase | |||
Region | 935-1057 | N-terminal hotdog fold | |||
Region | 935-1095 | Dehydratase | |||
Domain | 935-1207 | PKS/mFAS DH | |||
Region | 1069-1207 | C-terminal hotdog fold | |||
Region | 1400-1705 | Enoylreductase | |||
Region | 1718-1899 | Beta-ketoacyl reductase (KR) | |||
Domain | 2004-2079 | Carrier | |||
Region | 2081-2104 | Disordered | |||
Sequence similarities
Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,104
- Mass (Da)217,744
- Last updated2008-06-10 v1
- Checksum7A33F823206BDCA5
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000854 EMBL· GenBank· DDBJ | ACC40211.1 EMBL· GenBank· DDBJ | Genomic DNA |