B2GUX3 · B2GUX3_RAT
- ProteinDNA replication licensing factor MCM5
- GeneMcm5
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids734 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+This reaction proceeds in the forward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | CMG complex | |
Cellular Component | MCM complex | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | DNA replication origin binding | |
Molecular Function | helicase activity | |
Molecular Function | hydrolase activity | |
Molecular Function | single-stranded DNA binding | |
Biological Process | DNA replication initiation | |
Biological Process | DNA unwinding involved in DNA replication | |
Biological Process | double-strand break repair via break-induced replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA replication licensing factor MCM5
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionB2GUX3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Expression
Gene expression databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length734
- Mass (Da)82,466
- Last updated2008-06-10 v1
- ChecksumACBAD4B5DDD81DD9
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
E9PTS4 | E9PTS4_RAT | Mcm5 | 736 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC166442 EMBL· GenBank· DDBJ | AAI66442.1 EMBL· GenBank· DDBJ | mRNA |