B1YIS7 · SYI_EXIS2

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site551L-isoleucyl-5'-AMP (UniProtKB | ChEBI)
Binding site595ATP (UniProtKB | ChEBI)
Binding site881Zn2+ (UniProtKB | ChEBI)
Binding site884Zn2+ (UniProtKB | ChEBI)
Binding site901Zn2+ (UniProtKB | ChEBI)
Binding site904Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • Ordered locus names
      Exig_1951

Organism names

Accessions

  • Primary accession
    B1YIS7

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10001891661-911Isoleucine--tRNA ligase

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif57-67'HIGH' region
Motif592-596'KMSKS' region

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    911
  • Mass (Da)
    102,680
  • Last updated
    2008-05-20 v1
  • Checksum
    715DFFE7B370694A
MEYKDTLLMMKTEFLMRGNLPKREPDMQARWNEMNLYEAVQEKNAGKPTFILHDGPPYANGDIHMGHGLNKVLKDIVVRYKSMNGFRSPYVPGWDTHGLPIETALQKAGVDRKSMSVAEFRELCAKYALEQVDHQREQFKRLGVLGDYDNPYITLQPEFEAAQIRLFGDMANKGYIYKGKKPVYWSPSSESALAEAEIEYQDKRSAAIYVAFQVMDGKNILEPTDHFVIWTTTPWTIPANLGISVSGELTYARIEHEGKGYIVAETLVPEVIEALGWEGATIGRVFDGADFEYIKAKHPLYDRESLVMLGDHVTAEATGVVHTAPGHGEDDFRIGQAYGLDVLCPVDDKGVMTAEAPGFEGLFYEDANKEIGVALEEAGALLKLSFIKHSYPHDWRTKKPVIFRATPQWFASIKDFRAEILDEIKEVQWVPEWGETRLHNMFKDRGDWVISRQRAWGVPLPIFYAEDGTEIVTPETIDHIANLFAAHGSNVWYEREAVDLLPEGFTHPASPNGIFKKETDIMDVWFDSGSSHAGVLATRPELERPADLYLEGSDQYRGWFNSSLSTSVATTGKAPYKAVVSHGFVLDGQGRKMSKSIGNTIAPIQIMQQFGAEILRLWVASVDYQSDVRASMDNFKQVSESYRKIRNTVRFLLGNLDQFDHTTHRVAFKDLPESDRFMRTKLDQLVGKVKAAYDAYDFMAVYQLLHNFCVLDLSSFYLDYTKDILYIEKEDAPARRAVQTVMYDTVVTLLQLMAPVLPHTADEAWEFVPGVETASIFLTDLPEAPEMTEEGLALIAKWNSFLVFRDDVLKALEEARAEKLVGKTLEAKLLLAPNDETKALLATIDHLEQLLQVSQIEFVASAEKSYGTTGITVLKADGEKCERCWTYSTELGQDPAHPTLCPRCTEVVNSL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001022
EMBL· GenBank· DDBJ
ACB61403.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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