B1XK17 · TRMFO_PICP2
- ProteinMethylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
- GenetrmFO
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids456 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.
Catalytic activity
- uridine54 in tRNA + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADH + H+ = 5-methyluridine54 in tRNA + (6S)-5,6,7,8-tetrahydrofolate + NAD+
RHEA-COMP:10193 CHEBI:65315 Position: 54+ CHEBI:15636 + CHEBI:57945 + CHEBI:15378 = RHEA-COMP:10167 CHEBI:74447 Position: 54+ CHEBI:57453 + CHEBI:57540
Cofactor
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 12-17 | FAD (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | tRNA (uracil(54)-C5)-methyltransferase activity, 5,10-methylenetetrahydrofolate-dependent | |
Biological Process | tRNA methylation | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Oscillatoriophycideae > Chroococcales > Geminocystaceae > Picosynechococcus
Accessions
- Primary accessionB1XK17
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000346404 | 1-456 | Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO | ||
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length456
- Mass (Da)50,157
- Last updated2008-05-20 v1
- Checksum648349B095D44995
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000951 EMBL· GenBank· DDBJ | ACB00372.1 EMBL· GenBank· DDBJ | Genomic DNA |