B1PHJ3 · B1PHJ3_9ALPC

Function

function

Forms a primer, NSP9-pU, which is utilized by the polymerase for the initiation of RNA chains. Interacts with ribosome signal recognition particle RNA (SRP). Together with NSP8, suppress protein integration into the cell membrane, thereby disrupting host immune defenses.
RNA-directed RNA polymerase that catalyzes the transcription of viral genomic and subgenomic RNAs. Acts in complex with nsp7 and nsp8 to transcribe both the minus and positive strands of genomic RNA. The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the amino terminus of NSP9, forming a covalent RNA-protein intermediate that serves as transcription/replication primer. Subgenomic RNAs (sgRNAs) are formed by discontinuous transcription: The polymerase has the ability to pause at transcription-regulating sequences (TRS) and jump to the leader TRS, resulting in a major deletion. This creates a series of subgenomic RNAs that are replicated, transcribed and translated. In addition, Nsp12 is a subunit of the viral RNA capping enzyme that catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. Subsequently, the NiRAN domain transfers RNA to GDP, and forms the core cap structure GpppA-RNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site5865
Active site5867
Active site5966
Active site6042
Active site6047
Binding site6105-6111S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site6519
Active site6534
Active site6575

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type deubiquitinase activity
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processsymbiont-mediated perturbation of host ubiquitin-like protein modification
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity
Biological Processviral protein processing
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Gene names

    • Name
      ORF1ab

Organism names

  • Taxonomic identifier
  • Strain
    • AFCD62
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Alphacoronavirus > Minunacovirus

Accessions

  • Primary accession
    B1PHJ3

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane2124-2139Helical
Transmembrane2183-2203Helical
Transmembrane2268-2291Helical
Transmembrane2679-2701Helical
Transmembrane2937-2958Helical
Transmembrane2964-2984Helical
Transmembrane2991-3010Helical
Transmembrane3022-3044Helical
Transmembrane3456-3475Helical
Transmembrane3487-3504Helical
Transmembrane3511-3530Helical
Transmembrane3550-3568Helical
Transmembrane3580-3597Helical
Transmembrane3617-3635Helical
Transmembrane3647-3670Helical

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain2-109CoV Nsp1 globular
Domain112-351CoV Nsp2 N-terminal
Domain775-893CoV Nsp2 C-terminal
Domain894-990Ubiquitin-like
Domain1173-1423Peptidase C16
Domain1422-1589Macro
Domain1792-1847Ubiquitin-like
Domain1854-2111Peptidase C16
Domain2195-22593Ecto
Region2333-2423Y1
Domain2333-2672CoV Nsp3 Y
Region2337-2350ZF1
Region2383-2393ZF2
Region2424-2672CoV-Y
Region2571-2672Y4
Domain3052-3147Nsp4C
Domain3148-3449Peptidase C30
Domain3729-3811RdRp Nsp7 cofactor
Domain3812-4005RdRp Nsp8 cofactor
Domain4006-4115Nsp9 ssRNA-binding
Domain4116-4255ExoN/MTase coactivator
Domain4257-4506NiRAN
Domain4512-4610Nsp12 Interface
Domain4611-5178Nsp12 RNA-dependent RNA polymerase
Domain4858-5020RdRp catalytic
Domain5179-5262CV ZBD
Domain5436-5787+RNA virus helicase C-terminal
Domain5847-6061ExoN
Domain6070-6291N7-MTase
Region6182-6196GpppA-binding
Domain6294-6354Nsp15 N-terminal oligomerization
Domain6355-6472AV-Nsp11N/CoV-Nsp15M
Domain6489-6629NendoU
Domain6633-6929Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,932
  • Mass (Da)
    770,765
  • Last updated
    2008-04-29 v1
  • Checksum
    07CC4068322AA5D4

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EU420138
EMBL· GenBank· DDBJ
ACA52163.1
EMBL· GenBank· DDBJ
Genomic RNA

Genome annotation databases

Similar Proteins

Disclaimer

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