B1PHI7 · B1PHI7_9ALPC
- ProteinReplicase polyprotein 1a
- GeneORF1a
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids4324 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
Catalytic activity
- TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
- a 5'-end diphospho-ribonucleoside in mRNA + GTP + H+ = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphateThis reaction proceeds in the backward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell membrane | |
Cellular Component | host cell perinuclear region of cytoplasm | |
Cellular Component | membrane | |
Molecular Function | cysteine-type deubiquitinase activity | |
Molecular Function | cysteine-type endopeptidase activity | |
Molecular Function | omega peptidase activity | |
Molecular Function | RNA binding | |
Molecular Function | transferase activity | |
Molecular Function | zinc ion binding | |
Biological Process | induction by virus of host autophagy | |
Biological Process | proteolysis | |
Biological Process | symbiont-mediated perturbation of host ubiquitin-like protein modification | |
Biological Process | symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity | |
Biological Process | viral genome replication | |
Biological Process | viral protein processing | |
Biological Process | viral translational frameshifting | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameReplicase polyprotein 1a
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Alphacoronavirus > Minunacovirus > Miniopterus bat coronavirus 1
Accessions
- Primary accessionB1PHI7
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Host membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 2180-2195 | Helical | ||||
Sequence: SIIWFFTMLSIIFKAF | ||||||
Transmembrane | 2239-2259 | Helical | ||||
Sequence: FLKLSVIAYALYALSFMFVRF | ||||||
Transmembrane | 2324-2347 | Helical | ||||
Sequence: FLYLAFLFIFGGIFVKGLVCYFLA | ||||||
Transmembrane | 2735-2757 | Helical | ||||
Sequence: FIWWVCAAIITFFLCLSISEGLV | ||||||
Transmembrane | 2993-3014 | Helical | ||||
Sequence: VIFNCVVAAMAIFICFLVVKFK | ||||||
Transmembrane | 3020-3040 | Helical | ||||
Sequence: LSYGVCSVIAAVTINNLSYVF | ||||||
Transmembrane | 3047-3066 | Helical | ||||
Sequence: MFVYATFYFLAVRNLNYAWI | ||||||
Transmembrane | 3078-3100 | Helical | ||||
Sequence: LAPWFIIVWYVVTMLTGLLPSVL | ||||||
Transmembrane | 3512-3531 | Helical | ||||
Sequence: VFNNVILVSVFWSMFLSELL | ||||||
Transmembrane | 3543-3560 | Helical | ||||
Sequence: LITAVFVLLFGVAVMLTF | ||||||
Transmembrane | 3567-3586 | Helical | ||||
Sequence: LFLYTFLIPSVVISACYNLA | ||||||
Transmembrane | 3606-3628 | Helical | ||||
Sequence: FSMDIQGCFNILACIFVNAIHTW | ||||||
Transmembrane | 3640-3667 | Helical | ||||
Sequence: TYVLSLCVSVYNYWCCGDFLSLAMMVLL | ||||||
Transmembrane | 3673-3691 | Helical | ||||
Sequence: WYIGAFAYRFSVFVVNYMD | ||||||
Transmembrane | 3703-3726 | Helical | ||||
Sequence: VILFMYVLCGYLCCVYYGICYWFN |
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 2-109 | CoV Nsp1 globular | ||||
Sequence: QSNLVTLAFANDSEISAEGFCDVETAVYAFSVSAVNGFADCRFVAQGLENCLVGVEADDYVLCVVGDVQLKAYIAKFSHRPFNLRGWIVRSNSNYFLETMDLVFGCGA | ||||||
Domain | 112-356 | CoV Nsp2 N-terminal | ||||
Sequence: SIPVDNYMCGANGKPVITEDMWYFCDYFGDDGDKITINGQEYHKAWNVTRSDVPYQFQNAGTILSIEYVSTEAHVLPDGAIAKTAKPPKFSKNVVLSEKCKALYDACGSPFVTNGTNVLEVVTNPIFAHGFVQCKCGSKHWTTGDWSGFKSVCCGTPGRVLCTVFGGVTPGSILLTSTRVDATPGATRYYHGLTLKHICNVDDVACWRVVKVQAVAGFVVKGSLEECVSTFDTCTHDNFTTVAKA | ||||||
Domain | 893-989 | Ubiquitin-like | ||||
Sequence: SAVTFSEKVAVKQIDPVYKVKLIFEFEDDTISSVCKQAIGKYITFEGNDWSNFEETIHNAMSVVGEFVDLPDYYIYDEEGGNDLNNSVMISQWPMFD | ||||||
Domain | 1181-1431 | Peptidase C16 | ||||
Sequence: DLDDVNKALSFMMPQETKFVDPFKFDYFDHEGIRVLRQNNNNCWVATTLVQLQLSGLLDDDDSMALFKAGSVSPLVRKCYNATGAIMGSLGDSSQCLEVLLKDLHSMFITCDSTCGCGSGTYELSGSVFRFMPTRDSFDYGACTVCGKVLKLKIKTLVGTGIFCQDPKPFNTARAIVKPICASIYQGSTTSGHYKTNVYGKRFCVDGSGVSSICNGNVNTILLKDCNYGIPAEEPKQKEFEKFVTPDDVVQ | ||||||
Domain | 1433-1600 | Macro | ||||
Sequence: AQPKPKPFTTYDNIEFYQGDISDLVGLDFDFIVNAANENLKHAGGVAAAIDKLTGNELQSLSNKYVKANGQVKVGSGVMIRCKKYSVFNVVGPRKGKNAPTLLEKCYKTILHENGVPLTPLISVGIFGIPLATSFDALLNTSSGRTVRCFCYTDKECNEIKTLVSDRK | ||||||
Domain | 1848-1903 | Ubiquitin-like | ||||
Sequence: DTKTTVKVSLDGRNVVDVDVAADKTIGEQLNACTTDNVIISNSVVTDVIDTVVNVA | ||||||
Domain | 1910-2174 | Peptidase C16 | ||||
Sequence: TFYGFPNAAEFHMLDHSAYAFDSDVVDGKRALVGTDNNCWVNAVCLQLQFAEVDFTSEGLKDMWNEFLVGNVAKFCHWLYWLVRATKGDAGDAENALNMLAKYVKAHGTITITRETDDGCCANEHRISSFVVNASVLRSGCTDGYCKHGNAYTACVSKVDGVSVIVNVDRPSVMSDNLLLTGTSYTAFSGPMDSGHYRVYNPVTSKMFDGANCVGGDLCNLAVTAVVVKNKVFKMQTSNSNTPVKIVKKLDDASEKFFSFGDVVS | ||||||
Domain | 2251-2315 | 3Ecto | ||||
Sequence: ALSFMFVRFSPANEYFCKEHVEGYGNSTFVKDEYCASTMCKVCLFGYQELADLPHTKVVWKYVGF | ||||||
Region | 2389-2479 | Y1 | ||||
Sequence: MFIKHVCFGCDKPSCVACSKSARLTRVPMQTIVNGANKSFYVVANGGSSYCHKHKFFCLNCDSYGPGNTFINETVARELSNVVKTNVQPTG | ||||||
Domain | 2389-2728 | CoV Nsp3 Y | ||||
Sequence: MFIKHVCFGCDKPSCVACSKSARLTRVPMQTIVNGANKSFYVVANGGSSYCHKHKFFCLNCDSYGPGNTFINETVARELSNVVKTNVQPTGDSFIEVDKVSFENGFYYLYSGETFWRYNFDVTDAKYGCKEVLKNCNVLADFIVYNNTGSNVSQIRNACVYFSQMLCKPIKLVDATLLSTLNVDFNGALHSAFIQVLNDSFSKDLSSCASMTECKQALGFDVSDEEFVNAVSNAHRFNVLLSDNSFNNLLTSYAKPEEQLSTHDVATCMRFNAKVVNHNVLIKENVPIVWLARDFQQLSEEGRKYLVKTTKAKGVTFLLTFNSNAMNVKLPVISIVNKKG | ||||||
Region | 2393-2406 | ZF1 | ||||
Sequence: HVCFGCDKPSCVAC | ||||||
Region | 2439-2449 | ZF2 | ||||
Sequence: CHKHKFFCLNC | ||||||
Region | 2480-2728 | CoV-Y | ||||
Sequence: DSFIEVDKVSFENGFYYLYSGETFWRYNFDVTDAKYGCKEVLKNCNVLADFIVYNNTGSNVSQIRNACVYFSQMLCKPIKLVDATLLSTLNVDFNGALHSAFIQVLNDSFSKDLSSCASMTECKQALGFDVSDEEFVNAVSNAHRFNVLLSDNSFNNLLTSYAKPEEQLSTHDVATCMRFNAKVVNHNVLIKENVPIVWLARDFQQLSEEGRKYLVKTTKAKGVTFLLTFNSNAMNVKLPVISIVNKKG | ||||||
Region | 2627-2728 | Y4 | ||||
Sequence: VLLSDNSFNNLLTSYAKPEEQLSTHDVATCMRFNAKVVNHNVLIKENVPIVWLARDFQQLSEEGRKYLVKTTKAKGVTFLLTFNSNAMNVKLPVISIVNKKG | ||||||
Domain | 3108-3203 | Nsp4C | ||||
Sequence: LFEGDKFVGTFENAAFGTFVIDMHSYEKLVNSITPEKLKQHASMFNKYKYYSGSASEADYRCACFAHLAKAMTDYAANHQDMLYSPPSISYNSTLQ | ||||||
Domain | 3204-3505 | Peptidase C30 | ||||
Sequence: AGLRKFAQPSGVIEHCIVRVSYGNMVLNGIWLGDEVICPRHVIASSTNTTIDYDHEYTMMRLHNFSVSSGNLFIGVVSAKMRGASLVIKVNQNNPHTPKHVFKTLKAGDAFNILACYDGVPSGVYGTILRHNKTIRGSFINGACGSPGFNINGDTVEFVYLHQLELGSGCHVGSNMEGAMYGGFEDQPSLQIEGADCLVTVNVIAFLYGAILNGCTWFLSNERITAEVFNGWAHANNFTEVGSLDCFNILAAKTGVDIQRVLASIQKLSKGFGGRNIIGYASLTDEFTVTEVVKQMYGVSLQ | ||||||
Domain | 3785-3867 | RdRp Nsp7 cofactor | ||||
Sequence: SKLTDIKCTNVVLMGCLSSMNIEANSKKWSYCVDLHNKINLCDDAEKAMEYLLALVTFFISEHADFNVSELVDSYFGDNSILQ | ||||||
Domain | 3868-4061 | RdRp Nsp8 cofactor | ||||
Sequence: SVASTFVNMPSFMAYENARQSYEEAINNGSSPQLVKQLKRAMNIAKAELDHESSVQRKLNRMAEQAAAQMYKEARAVNKKSKVISSLHTLLFGMLRKLDMSSVDNILSLARDGVVPLSIIPAACATKLTIVVSDFESFKRIFQLGNVQYAGVVWSLIEVKDNDGKPVHVKEITATNTALTWPLILNCERVVKLQ | ||||||
Domain | 4062-4171 | Nsp9 ssRNA-binding | ||||
Sequence: NNEVIPGKLNVRPVKGEGDGGFTADGKALFNNEGGKTFMYAFIADKPDLKVVKWEFDGGCNVIELEPPCKFAVVDAGGNNVIKYLYFVKNLNTLRRGAVLGFIGATVRLQ | ||||||
Domain | 4172-4311 | ExoN/MTase coactivator | ||||
Sequence: AGKQTELAVNSSLLTLCSFAVDPAKCYLYAVKSGVKPINNCVKMLSNGSGTGQAVTVGVEATTNQDSYGGASVCLYCRAHVDHPSIDGFCQFKGRYVQVPVGTVDPIRFCLENQVCKVCHCWLNNGCSCDRTAVVQSMDH |
Sequence similarities
Belongs to the coronaviruses polyprotein 1ab family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length4,324
- Mass (Da)476,631
- Last updated2008-04-29 v1
- Checksum4C75D27EBA9CF1B6
Keywords
- Coding sequence diversity