B1AS29 · GRIK3_MOUSE
- ProteinGlutamate receptor ionotropic, kainate 3
- GeneGrik3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids919 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (PubMed:17620617, PubMed:20007474).
In association with GRIK2, involved in presynaptic facilitation of glutamate release at hippocampal mossy fiber synapses (PubMed:17620617).
In association with GRIK2, involved in presynaptic facilitation of glutamate release at hippocampal mossy fiber synapses (PubMed:17620617).
Miscellaneous
The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate >> L-glutamate = quisqualate >> AMPA = NMDA.
Catalytic activity
- Ca2+(in) = Ca2+(out)
Activity regulation
Glutamate-gated receptor activity inhibited by spermine.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 518 | L-glutamate (UniProtKB | ChEBI) | ||||
Sequence: P | ||||||
Binding site | 520 | L-glutamate (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 525 | L-glutamate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 691 | L-glutamate (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 692 | L-glutamate (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 739 | L-glutamate (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamate receptor ionotropic, kainate 3
- Short namesGluK3
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionB1AS29
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Postsynaptic cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 32-563 | Extracellular | ||||
Sequence: MPHVIRIGGIFEYADGPNAQVMNAEEHAFRFSANIINRNRTLLPNTTLTYDIQRIHFHDSFEATKKACDQLALGVVAIFGPSQGSCTNAVQSICNALEVPHIQLRWKHHPLDNKDTFYVNLYPDYASLSHAILDLVQSLKWRSATVVYDDSTGLIRLQELIMAPSRYNIRLKIRQLPIDSDDSRPLLKEMKRGREFRIIFDCSHTMAAQILKQAMAMGMMTEYYHFIFTTLDLYALDLEPYRYSGVNLTGFRILNVDNPHVSAIVEKWAMERLQAAPRAESGLLDGVMMTDAALLYDAVHIVSVCYQRAPQMTVNSLQCHRHKAWRFGGRFMNFIKEAQWEGLTGRIVFNKTSGLRTDFDLDIISLKEDGLEKVGVWSPADGLNITEVAKGRGPNVTDSLTNRSLIVTTVLEEPFVMFRKSDRTLYGNDRFEGYCIDLLKELAHILGFSYEIRLVEDGKYGAQDDKGQWNGMVKELIDHKADLAVAPLTITHVREKAIDFSKPFMTLGVSILYRKPNGTNPSVFSFLNPLSP | ||||||
Transmembrane | 564-584 | Helical | ||||
Sequence: DIWMYVLLAYLGVSCVLFVIA | ||||||
Topological domain | 585-636 | Cytoplasmic | ||||
Sequence: RFSPYEWYDAHPCNPGSEVVENNFTLLNSFWFGMGSLMQQGSELMPKALSTR | ||||||
Transmembrane | 637-657 | Helical | ||||
Sequence: IIGGIWWFFTLIIISSYTANL | ||||||
Topological domain | 658-820 | Extracellular | ||||
Sequence: AAFLTVERMESPIDSADDLAKQTKIEYGAVKDGATMTFFKKSKISTFEKMWAFMSSKPSALVKNNEEGIQRTLTADYALLMESTTIEYITQRNCNLTQIGGLIDSKGYGIGTPMGSPYRDKITIAILQLQEEDKLHIMKEKWWRGSGCPEEENKEASALGIQK | ||||||
Transmembrane | 821-841 | Helical | ||||
Sequence: IGGIFIVLAAGLVLSVLVAVG | ||||||
Topological domain | 842-919 | Cytoplasmic | ||||
Sequence: EFIYKLRKTAEREQRSFCSTVADEIRFSLTCQRRLKHKPQPPMMVKTDAVINMHTFNDRRLPGKDSMSCSTSLAPVFP |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 618 | Loss of spermine-binding; when associated with A-620. | ||||
Sequence: M → V | ||||||
Mutagenesis | 620 | Loss of spermine-binding; when associated with V-618. | ||||
Sequence: S → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 37 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for signal, chain, glycosylation, disulfide bond, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-31 | |||||
Sequence: MTAPWRRLRSLVWEYWAGFLVCAFWIPDSRG | ||||||
Chain | PRO_0000416793 | 32-919 | Glutamate receptor ionotropic, kainate 3 | |||
Sequence: MPHVIRIGGIFEYADGPNAQVMNAEEHAFRFSANIINRNRTLLPNTTLTYDIQRIHFHDSFEATKKACDQLALGVVAIFGPSQGSCTNAVQSICNALEVPHIQLRWKHHPLDNKDTFYVNLYPDYASLSHAILDLVQSLKWRSATVVYDDSTGLIRLQELIMAPSRYNIRLKIRQLPIDSDDSRPLLKEMKRGREFRIIFDCSHTMAAQILKQAMAMGMMTEYYHFIFTTLDLYALDLEPYRYSGVNLTGFRILNVDNPHVSAIVEKWAMERLQAAPRAESGLLDGVMMTDAALLYDAVHIVSVCYQRAPQMTVNSLQCHRHKAWRFGGRFMNFIKEAQWEGLTGRIVFNKTSGLRTDFDLDIISLKEDGLEKVGVWSPADGLNITEVAKGRGPNVTDSLTNRSLIVTTVLEEPFVMFRKSDRTLYGNDRFEGYCIDLLKELAHILGFSYEIRLVEDGKYGAQDDKGQWNGMVKELIDHKADLAVAPLTITHVREKAIDFSKPFMTLGVSILYRKPNGTNPSVFSFLNPLSPDIWMYVLLAYLGVSCVLFVIARFSPYEWYDAHPCNPGSEVVENNFTLLNSFWFGMGSLMQQGSELMPKALSTRIIGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVKDGATMTFFKKSKISTFEKMWAFMSSKPSALVKNNEEGIQRTLTADYALLMESTTIEYITQRNCNLTQIGGLIDSKGYGIGTPMGSPYRDKITIAILQLQEEDKLHIMKEKWWRGSGCPEEENKEASALGIQKIGGIFIVLAAGLVLSVLVAVGEFIYKLRKTAEREQRSFCSTVADEIRFSLTCQRRLKHKPQPPMMVKTDAVINMHTFNDRRLPGKDSMSCSTSLAPVFP | ||||||
Glycosylation | 70 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 76 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 99↔350 | |||||
Sequence: CDQLALGVVAIFGPSQGSCTNAVQSICNALEVPHIQLRWKHHPLDNKDTFYVNLYPDYASLSHAILDLVQSLKWRSATVVYDDSTGLIRLQELIMAPSRYNIRLKIRQLPIDSDDSRPLLKEMKRGREFRIIFDCSHTMAAQILKQAMAMGMMTEYYHFIFTTLDLYALDLEPYRYSGVNLTGFRILNVDNPHVSAIVEKWAMERLQAAPRAESGLLDGVMMTDAALLYDAVHIVSVCYQRAPQMTVNSLQC | ||||||
Glycosylation | 278 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 381 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 415 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 426 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 433 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 548 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 551 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 752 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 869 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 887 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Detected in whole brain, cerebellum, brain cortex and hippocampus.
Gene expression databases
Interaction
Subunit
Homotetramer, and heterotetramer with either GRIK4 or GRIK5 (By similarity).
Can form functional heteromeric receptors with GRIK2 (PubMed:17620617).
Interacts with PRKCABP (By similarity).
Interacts with NETO2 (By similarity).
Can form functional heteromeric receptors with GRIK2 (PubMed:17620617).
Interacts with PRKCABP (By similarity).
Interacts with NETO2 (By similarity).
Protein-protein interaction databases
Miscellaneous
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length919
- Mass (Da)104,051
- Last updated2008-04-08 v1
- ChecksumDD543E45951ACB87
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL607090 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL627328 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC118004 EMBL· GenBank· DDBJ | AAI18005.1 EMBL· GenBank· DDBJ | mRNA | ||
AF245444 EMBL· GenBank· DDBJ | AAF90049.1 EMBL· GenBank· DDBJ | mRNA |