B0XMC1 · ER10A_ASPFC

Function

function

Mitochondrial acetyl-CoA acetyltransferase that catalyzes both the formation and degradation of acetoacetyl-CoA (PubMed:32005728).
Has no overlapping function with erg10B and seems not to be involved in ergosterol biosynthesis (PubMed:32005728).
Plays an important role in growth, morphogenesis and maintaining mitochondrial function including the response to oxidative stresses (PubMed:32005728).

Miscellaneous

Even though it is not involved in catalysis directly, the Cl- anion stabilizes the catalytic site via both direct and water-mediated hydrogen bonds to catalytic residues and residues adjacent.

Catalytic activity

Cofactor

K+ (UniProtKB | Rhea| CHEBI:29103 )

Biotechnology

Mitochondrial erg10A is essential for A.fumigatus cell viability and might be a potential drug target to feed the antifungal drug development pipeline.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
26 μMCoA
43 μMacetoacetyl-CoA
232 μMacetyl-CoA

Pathway

Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site124Acyl-thioester intermediate
Binding site219K+ (UniProtKB | ChEBI)
Binding site229CoA (UniProtKB | ChEBI)
Binding site262CoA (UniProtKB | ChEBI)
Binding site280K+ (UniProtKB | ChEBI)
Binding site284CoA (UniProtKB | ChEBI)
Active site387Proton acceptor
Active site415Proton acceptor
Binding site416chloride (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmitochondrion
Molecular Functionacetyl-CoA C-acyltransferase activity
Molecular Functionmetal ion binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acetyl-CoA acetyltransferase erg10A, mitochondrial
  • EC number
  • Alternative names
    • Acetoacetyl-CoA thiolase erg10A
    • Ergosterol biosynthesis protein 10A

Gene names

    • Name
      erg10A
    • ORF names
      AFUB_000550

Organism names

Accessions

  • Primary accession
    B0XMC1

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Leads to lethality.

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-34Mitochondrion
ChainPRO_000045414535-433Acetyl-CoA acetyltransferase erg10A, mitochondrial

Interaction

Subunit

Homotetramer.

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    433
  • Mass (Da)
    45,710
  • Last updated
    2008-04-08 v1
  • Checksum
    4D5B4C442DAAABA0
MAIQTTTGLAARLVAKRATFPASRRNFSASRSALKEIQEAYILSGARTPTAKFNGSFVSVSAPELGAVAIKSAVSKSGVPVEKITDVYMGNVLQGAVGQAPARQASMFAGLSPTVESMTVNKVCASGLKAVALAAQNIQLGLAEAQVAGGMENMSRVPYYLPRSTQLPPFGEIKLQDGLIQDGLWDVYNQFHMGICAEKTAKKYEISREEQDQYAIQSYQRAQAAWKENKFAEEIAPVTVKGKKGETVVERDEGYENLRIDKMATLKPAFLRDGTGTVTAGNASTMNDGASALVLGSKAIAREFAQGNRALARIVSTADAAIDPVDFPVAPAKAVPIALERAGITKDQVAVWEFNEAFAAVIKANEKILGLQNARVNPLGGAISLGHALGSSGSRILVTLLHQLQPGEYGVAAICNGGGAATAMVVQKLDRVD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DS499594
EMBL· GenBank· DDBJ
EDP55365.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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