B0SGA8 · RLMN_LEPBA

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site95Proton acceptor
Binding site117[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site121[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site124[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site164-165S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site196S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site219-221S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site296S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site339S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Probable dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • Ordered locus names
      LBF_1312

Organism names

Accessions

  • Primary accession
    B0SGA8

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00003502301-353Probable dual-specificity RNA methyltransferase RlmN
Disulfide bond110↔339(transient)

Keywords

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain103-333Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    353
  • Mass (Da)
    39,889
  • Last updated
    2008-04-08 v1
  • Checksum
    5B52326C4999C028
MKEEIPVLKGKTKKELEEICVSLGLEKYRAAQIYTGIYKSRYTTIDQFTTLSKEVREKLKEHTQYPEIEIGRDLVSKEDGTRKFTFYVGENKEIEAVWIPSGDGGRKTICISSQIGCTLNCKFCATGLLEYKGNLQTWQILDQVLQVERLVGDRATNIVFMGMGEPMHNYFSVMKAAHILRDKDAFGLGALRITISTAGVTTGINRFIENKEPFNFAISLNHPNPNARSSVMDVNDKHPLEKLIDSAKRFTKELDRAITFEYVMIPDVNMGRDNAERLAKIARSVNKCKINVIPLNTDFTGWRRPTDDEVKDFVMHLKAKTTAPILNRRSPGRDINGACGMLALKGIRSETTK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000777
EMBL· GenBank· DDBJ
ABZ93832.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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