B0R4K0 · CHEB_HALS3
- ProteinProtein-glutamate methylesterase/protein-glutamine glutaminase
- GenecheB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids347 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the Htr transducer proteins (methyl-accepting chemotaxis proteins) by CheR. Also required for Htr deamidations, at least at a specific glutamine-glutamate pair in HTR-II and a specific aspartate-glutamine pair in Htr4.
Catalytic activity
- [protein]-L-glutamate 5-O-methyl ester + H2O = L-glutamyl-[protein] + methanol + H+
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 164 | |||||
Sequence: S | ||||||
Active site | 191 | |||||
Sequence: H | ||||||
Active site | 288 | |||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | phosphorelay response regulator activity | |
Molecular Function | protein-glutamate methylesterase activity | |
Molecular Function | protein-glutamine glutaminase activity | |
Biological Process | chemotaxis | |
Biological Process | protein deamination | |
Biological Process | protein demethylation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein-glutamate methylesterase/protein-glutamine glutaminase
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageArchaea > Euryarchaeota > Stenosarchaea group > Halobacteria > Halobacteriales > Halobacteriaceae > Halobacterium > Halobacterium salinarum NRC-34001
Accessions
- Primary accessionB0R4K0
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Deletion leads to the loss of both chemotactic and phototactic responses. Mutants show increased methylation of the Htr transducer proteins.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000429068 | 1-347 | Protein-glutamate methylesterase/protein-glutamine glutaminase | |||
Sequence: MTEALVVDDSHFMRTVISDILEDGGVDVVGTAENGARALDAVTDVQPDVITMDVEMPEMDGIEATAEIMREQPTPILMVSALTTEDADATLEAMEKGAIDTFAKPGGTISTELSGHSEELVAAVERVASADPTAGHDVEMEPASPPDATTSEYADNPTLLIGASTGGPNVVESILASLPAEADFRVLIVQHMPDQFTSRFADRLDAASQYDITEAEDGSRIGGGEGLVARGDYHMRVSGYSNGRLRVRLDQSERLHSVRPAIDVTFKSAAERVTDPLVSVVLTGMGSDGADGVRAVKDAGGATLAQNEATSAVFGIPERAIETGCVDDVLPVDQLTEAIADSIRRTT | ||||||
Modified residue | 53 | 4-aspartylphosphate | ||||
Sequence: D |
Post-translational modification
Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 3-119 | Response regulatory | ||||
Sequence: EALVVDDSHFMRTVISDILEDGGVDVVGTAENGARALDAVTDVQPDVITMDVEMPEMDGIEATAEIMREQPTPILMVSALTTEDADATLEAMEKGAIDTFAKPGGTISTELSGHSEE | ||||||
Region | 132-154 | Disordered | ||||
Sequence: PTAGHDVEMEPASPPDATTSEYA | ||||||
Domain | 152-346 | CheB-type methylesterase | ||||
Sequence: EYADNPTLLIGASTGGPNVVESILASLPAEADFRVLIVQHMPDQFTSRFADRLDAASQYDITEAEDGSRIGGGEGLVARGDYHMRVSGYSNGRLRVRLDQSERLHSVRPAIDVTFKSAAERVTDPLVSVVLTGMGSDGADGVRAVKDAGGATLAQNEATSAVFGIPERAIETGCVDDVLPVDQLTEAIADSIRRT |
Domain
Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.
Sequence similarities
Belongs to the CheB family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length347
- Mass (Da)36,484
- Last updated2008-04-08 v1
- Checksum9691873DC8EA113B
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 295 | in Ref. 1; CAA60162 | ||||
Sequence: A → P |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X86407 EMBL· GenBank· DDBJ | CAA60162.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AM774415 EMBL· GenBank· DDBJ | CAP13665.1 EMBL· GenBank· DDBJ | Genomic DNA |