B0JVA7 · ISPH_MICAN

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site66[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site96(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site96dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site96isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site157[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site185(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site185dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site185isopentenyl diphosphate (UniProtKB | ChEBI)
Active site187Proton donor
Binding site250(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site288[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site317(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site317dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site317isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site318(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site318dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site318isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site319(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site319dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site319isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site379(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site379dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site379isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • Ordered locus names
      MAE_16190

Organism names

Accessions

  • Primary accession
    B0JVA7

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000775191-4024-hydroxy-3-methylbut-2-enyl diphosphate reductase

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the IspH family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    402
  • Mass (Da)
    45,595
  • Last updated
    2008-03-18 v1
  • Checksum
    CA57697354F754CB
MDTKSFKRTLQQSDNYNRKGFGHKEEVMDAMTNEYQSDLIQEIRENNYRLQRGDVTIYLAQAFGFCWGVERAVAMAYETRQHFPQERIWITNEIIHNPSVNQRLRSMAVGFIPVENGQKDFTVVESGDVVILPAFGASVSEMQILNDKGCMIVDTTCPWVSKVWNSVEKHKKSDHTSIIHGKYNHEETIATSSFAGTYLIVLNLDQANYVANYILHGGDKNEFLEKFKNAHSQGFDPDRDLDYIGIANQTTMLKSETEEIGKLFEHTMLRKYGPIDFKDHFMSFNTICDATQERQDAIFELVKEPLSLMVVIGGYNSSNTTHLQEIAIERAIPSYHIDSAERILPGNRIEHKPLGDDLIITDNWLNEGKIVVGVTSGASTPDKVVEEVIEKIFAFKSSLVPG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP009552
EMBL· GenBank· DDBJ
BAG01441.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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