B0CYJ4 · B0CYJ4_LACBS
- Protein5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids765 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
Cofactor
Note: Binds 2 Zn2+ ions per subunit.
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 19 | 5-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 120 | 5-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 441-443 | L-homocysteine (UniProtKB | ChEBI) | ||||
Sequence: IGS | ||||||
Binding site | 441-443 | L-methionine (UniProtKB | ChEBI) | ||||
Sequence: IGS | ||||||
Binding site | 494 | L-methionine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 571 | 5-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 609 | L-homocysteine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 609 | L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 651 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: H | ||||||
Binding site | 653 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: C | ||||||
Binding site | 675 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: E | ||||||
Active site | 704 | Proton donor | ||||
Sequence: H | ||||||
Binding site | 736 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity | |
Molecular Function | zinc ion binding | |
Biological Process | methionine biosynthetic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Basidiomycota > Agaricomycotina > Agaricomycetes > Agaricomycetidae > Agaricales > Agaricineae > Hydnangiaceae > Laccaria
Accessions
- Primary accessionB0CYJ4
Proteomes
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 4-311 | Cobalamin-independent methionine synthase MetE N-terminal | ||||
Sequence: SSVLGFPRIGANREVKKAVEAYWAEKFTADELTKVAADVKRASWTSVKAQGVDFIPSGEFSLYDHVLDHSAAFNVIPKRYVGLGLSPLDVYFAMGRGRQSEVVDVPASEMKKWFDSNYHFVVPEFSDETDFKLNFNKALLEFKEAQAAGVTTRPVVLGPISFLTLGKASKDAKPGFQPISLLPKLLPIYKTLLTDLKQAGAEWVQVDEPILVLDTAAALENEFATAYAELAPVAPKILLTTYFSRLDSNLTFIAKLPVAGLHIDLDRAPAQLDEVISAVKSTPIILSLGVVSGRNIWKTDFAAAIKLG | ||||||
Domain | 436-758 | Cobalamin-independent methionine synthase MetE C-terminal/archaeal | ||||
Sequence: FPTTTIGSFPQTKEIRQARAKLGKGELTEEQYEEFIKKEIGAVVRFQEKIGLDLLVHGEPERNDMVQYFGEQLHGFVFTQNAWVQSYGSRYVRPPIIVSDVSRPGPMTVKWSSYAQSLTSKPMKGMLTGPVTILNWSFPRADVSRELQSKQLALALRDEVVDLEKAGISAIQVDEPAIREGLPLRRSDWDAYLKWAVDSFKLSTAGVTDQLQTHSHFCYSDFDDIFPSIQRLDADVISIEASKADMKLLNTFKHYGYSNQIGPGVYDIHSPRVPGGQEIKDRLKSMLDILPDSLLFVNPDCGLKTRGWKETEASLINLVEGAK |
Sequence similarities
Belongs to the vitamin-B12 independent methionine synthase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length765
- Mass (Da)84,730
- Last updated2008-02-26 v1
- ChecksumEE8F54D80031AC1D
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DS547094 EMBL· GenBank· DDBJ | EDR12893.1 EMBL· GenBank· DDBJ | Genomic DNA |