B0CJ70 · THIG_BRUSI
- ProteinThiazole synthase
- GenethiG
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids256 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.
Catalytic activity
- 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH = 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + 2 H+ + 2 H2O
Pathway
Cofactor biosynthesis; thiamine diphosphate biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 96 | Schiff-base intermediate with DXP | ||||
Sequence: K | ||||||
Binding site | 157 | 1-deoxy-D-xylulose 5-phosphate (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 184-185 | 1-deoxy-D-xylulose 5-phosphate (UniProtKB | ChEBI) | ||||
Sequence: AG | ||||||
Binding site | 206-207 | 1-deoxy-D-xylulose 5-phosphate (UniProtKB | ChEBI) | ||||
Sequence: NT |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | thiazole synthase activity | |
Biological Process | thiamine diphosphate biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThiazole synthase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Brucellaceae > Brucella/Ochrobactrum group > Brucella
Accessions
- Primary accessionB0CJ70
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000080866 | 1-256 | Thiazole synthase | |||
Sequence: MLEFYGKRFESRLLLGTAQYPSPSILADAVRASLSRTVTVSLRRESGEARAGQDFWALIKALGVAVLPNTAGCHTPREAITTAHMAREVFGTNWIKLEVIGDTDTLQPDPFGLVEAARILCDEGFEVFPYMNDDLIVAERLIEAGCKVLMPWGAPIGSGRGLNNPYALKTMRAHFPDIPLVVDAGIGVPSHAAAAMELGFDAVLINTAVAKAGDPAAMARAFALAVEAGRLAYEADPIEARDMASPSTPLLGKAFL |
Interaction
Subunit
Homotetramer. Forms heterodimers with either ThiH or ThiS.
Structure
Sequence
- Sequence statusComplete
- Length256
- Mass (Da)27,308
- Last updated2008-02-26 v1
- Checksum11CA52B882801731
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000911 EMBL· GenBank· DDBJ | ABY37314.1 EMBL· GenBank· DDBJ | Genomic DNA |