B0B9V8 · KDTA_CHLT2
- Protein3-deoxy-D-manno-octulosonic acid transferase
- GenewaaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids431 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of three 3-deoxy-D-manno-octulosonate (Kdo) residues from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A. Thus generates the genus-specific LPS epitope of Chlamydia, composed of the trisaccharide alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo.
Catalytic activity
- CMP-3-deoxy-beta-D-manno-octulosonate + lipid IVA (E. coli) = alpha-Kdo-(2->6)-lipid IVA (E. coli) + CMP + H+
Pathway
Bacterial outer membrane biogenesis; LPS core biosynthesis.
Features
Showing features for active site, site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 67 | Proton acceptor | ||||
Sequence: E | ||||||
Site | 138 | Transition state stabilizer | ||||
Sequence: E | ||||||
Site | 216 | Transition state stabilizer | ||||
Sequence: K | ||||||
Binding site | 275-276 | CMP (UniProtKB | ChEBI) | ||||
Sequence: PR | ||||||
Binding site | 315-317 | CMP (UniProtKB | ChEBI) | ||||
Sequence: MGV | ||||||
Binding site | 342-345 | CMP (UniProtKB | ChEBI) | ||||
Sequence: NLLE |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | Kdo transferase activity | |
Biological Process | lipopolysaccharide core region biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended name3-deoxy-D-manno-octulosonic acid transferase
- EC number
- Short namesKdo transferase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Chlamydiota > Chlamydiia > Chlamydiales > Chlamydiaceae > Chlamydia/Chlamydophila group > Chlamydia
Accessions
- Primary accessionB0B9V8
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 5-27 | Helical; Signal-anchor | ||||
Sequence: WLTSRLYDAFLVCAFFVSAPRIF |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000391796 | 1-431 | 3-deoxy-D-manno-octulosonic acid transferase | |||
Sequence: MIRRWLTSRLYDAFLVCAFFVSAPRIFYKVFFHGKYIDSWKIRFGVQKPFVKGEGPLVWFHGASVGEVSLLAPLLNRWREEFPEWRFVVTTCSEAGVHTARRLYESLGATVFVLPLDLSCIIKSVVRKLAPDIVIFSEGDCWLHFLTESKRLGAKAFLINGKLSEHSCKRFSFLKRLGRNYFAPLDLLILQDELYKQRFMQIGISSDKIHVTGNMKTFIESSLATNRRDFWRAKLQISSQDRLIVLGSMHPKDVEVWAEVVSHFHNSSTKILWVPRHLEKLKEHAKLLEKAGILFGLWSQGASFRQYNSLIMDAMGVLKDIYSAADIAFVGGTFDPSVGGHNLLEPLQKEAPLMFGPYIYSQSVLAERLREKEAGLSVNKETLLDVVTDLLQNEKNRQAYIEKGKSFLKQEENSFQQTWEILKSQITCMKI |
Structure
Family & Domains
Sequence similarities
Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 30 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length431
- Mass (Da)49,497
- Last updated2008-02-26 v1
- Checksum664A7504F4E749AC
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 301 | in Ref. 1; AAA23139 and 2; CAA80374 | ||||
Sequence: G → A | ||||||
Sequence conflict | 397-431 | in Ref. 1; AAA23139 | ||||
Sequence: RQAYIEKGKSFLKQEENSFQQTWEILKSQITCMKI → PPSLY |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M64618 EMBL· GenBank· DDBJ | AAA23139.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z22659 EMBL· GenBank· DDBJ | CAA80374.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AM884176 EMBL· GenBank· DDBJ | CAP03899.1 EMBL· GenBank· DDBJ | Genomic DNA |