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B0B9U3 · TGT_CHLT2

Function

function

Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Pathway

tRNA modification; tRNA-queuosine biosynthesis.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site89Proton acceptor
Binding site89-93substrate
Binding site161substrate
Binding site232substrate
Active site281Nucleophile
Binding site319Zn2+ (UniProtKB | ChEBI)
Binding site321Zn2+ (UniProtKB | ChEBI)
Binding site324Zn2+ (UniProtKB | ChEBI)
Binding site351Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionmetal ion binding
Molecular FunctiontRNA-guanosine(34) queuine transglycosylase activity
Biological Processqueuosine biosynthetic process
Biological ProcesstRNA-guanine transglycosylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Queuine tRNA-ribosyltransferase
  • EC number
  • Alternative names
    • Guanine insertion enzyme
    • tRNA-guanine transglycosylase

Gene names

    • Name
      tgt
    • Ordered locus names
      CTL0445

Organism names

Accessions

  • Primary accession
    B0B9U3

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000975341-372Queuine tRNA-ribosyltransferase

Interaction

Subunit

Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region262-268RNA binding
Region286-290RNA binding; important for wobble base 34 recognition

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    372
  • Mass (Da)
    40,906
  • Last updated
    2008-02-26 v1
  • MD5 Checksum
    DE111852D4DA17970533D0C4B457AA20
MALRFEILHQSKKSRARVGRIETAHGYIDTPAFVPVATNGALKGVLDHSNIPLMFCNTYHLIVHPGAEAIAAMGGLHQFIGRNAPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGGNTIIKVNDDGVHFKSYRDGRKLFLSPEISVQAQKDLGADIILPLDELLPFHADPTYFHQSSQRTYVWEKRSLDYHLKNPGIQSMYGVIHGGTFPDQRKLGCKFVEDLPFDGSAIGGSLGKNLQDIVEVVGVTAANLSAERPRHLLGIGDLPSIWATVGFGIDSFDSSYPTKAARHGMILTSQGPLKINNQRYSSDLNPIEPGCSCLACSQGITRAYLRHLFKVHEPNAGIWASIHNMHHMQKVMREIREGILNDRI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AM884176
EMBL· GenBank· DDBJ
CAP03884.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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