A9TXT1 · CERK1_PHYPA
- ProteinChitin elicitor receptor kinase 1
- GeneCERK1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids640 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Lysin motif (LysM) receptor kinase required as a cell surface receptor for chitin elicitor (chitooligosaccharides) signaling leading to innate immunity in response to biotic stresses (By similarity).
The CERK1, MEKK1a/b, MKK1a/b/c and MPK4a/b proteins are involved in pathogen defense. The pathway induces rapid growth inhibition, cell wall depositions and accumulation of defense-related transcripts. This protein is required for response to chitin. Is able to complement the A.thaliana cerk1 mutant (PubMed:27268428).
The CERK1, MEKK1a/b, MKK1a/b/c and MPK4a/b proteins are involved in pathogen defense. The pathway induces rapid growth inhibition, cell wall depositions and accumulation of defense-related transcripts. This protein is required for response to chitin. Is able to complement the A.thaliana cerk1 mutant (PubMed:27268428).
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 119-125 | chitin (UniProtKB | ChEBI) | ||||
Sequence: TNDTAEK | ||||||
Binding site | 148-154 | chitin (UniProtKB | ChEBI) | ||||
Sequence: DLSSIYS | ||||||
Binding site | 336-344 | ATP (UniProtKB | ChEBI) | ||||
Sequence: IGQGGFASV | ||||||
Binding site | 357 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Active site | 452 | Proton acceptor | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | chitin binding | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Molecular Function | transmembrane receptor protein kinase activity | |
Biological Process | innate immune response |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameChitin elicitor receptor kinase 1
- EC number
- Short namesPpCERK1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Bryophyta > Bryophytina > Bryopsida > Funariidae > Funariales > Funariaceae > Physcomitrium
Accessions
- Primary accessionA9TXT1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Single-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 32-248 | Extracellular | ||||
Sequence: RETGVLIVDADCIPPNGCKALAYYRLKQGDDLEKLQGRFQTNNSEVLAYNPQLVDANSIQAGTNIYLPFDCLCLNGELVHRFSYTVTTNDTAEKVVDVTYQKLTTVGAVRSASNSGDLSSIYSGQSLTIPVRCYCGDPNVDPKYGLFSTYVVQADDQLTSLSTNFSVDADVISKFNSDTRNLSPDSIIFIPSKAANGSFPPFSGYVLGTVHWRSNVG | ||||||
Transmembrane | 249-269 | Helical | ||||
Sequence: IIVGVVVGGIVLAVLLLFALI | ||||||
Topological domain | 270-640 | Cytoplasmic | ||||
Sequence: FGFKHFRRRKLAKEPTMQQSGLLSSSSMAGSKPSRSGSTMLPVPKSVEFTYEELAAATDNFSLAKKIGQGGFASVYYGVIRDQKLAIKKMTLQCTKEFLAELQVLTNVHHTNLVQLIGYCTTNSLFLVYEYIENGTLDHHLRRRKSDDKPPLSWLQRVQICLDSARGLEYIHEHTKPTYIHRDIKSANILLDDNFRAKVADFGLAKLAEEGTGTGIVGTFGYMPPEYALYGEVSPKLDVYAFGVVLFEIISGRVAISSALPSENDQQSPAQNRESRTLTSFFEPVLNDPDGKTLLPKCIDPALNGEYSLDAVWKMAQLARRCTHQSPDMRPTMRFAVVQLMTLASVTQEWDVGYFSRASSQSQPPSGNDQL |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Chitin treatment does not lead to growth inhibition or reduced cell wall-associated depositions in contrast to the wild-type. No chitin-, chitosan- or chitin hexamer-induced MPK phosphorylation. Peptidyl glycan (PGN) from S.aureus does not induce MPK phosphorylation either. Reduced accumulation of PAL4 and CHS transcripts in response to chitin. Tissues fail to exhibit enhanced conductivity in an electrolyte leakage assay.
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-31 | |||||
Sequence: MKFQMKMKSELCRTYKYWLILLVLWLSGVTQ | ||||||
Chain | PRO_0000443381 | 32-640 | Chitin elicitor receptor kinase 1 | |||
Sequence: RETGVLIVDADCIPPNGCKALAYYRLKQGDDLEKLQGRFQTNNSEVLAYNPQLVDANSIQAGTNIYLPFDCLCLNGELVHRFSYTVTTNDTAEKVVDVTYQKLTTVGAVRSASNSGDLSSIYSGQSLTIPVRCYCGDPNVDPKYGLFSTYVVQADDQLTSLSTNFSVDADVISKFNSDTRNLSPDSIIFIPSKAANGSFPPFSGYVLGTVHWRSNVGIIVGVVVGGIVLAVLLLFALIFGFKHFRRRKLAKEPTMQQSGLLSSSSMAGSKPSRSGSTMLPVPKSVEFTYEELAAATDNFSLAKKIGQGGFASVYYGVIRDQKLAIKKMTLQCTKEFLAELQVLTNVHHTNLVQLIGYCTTNSLFLVYEYIENGTLDHHLRRRKSDDKPPLSWLQRVQICLDSARGLEYIHEHTKPTYIHRDIKSANILLDDNFRAKVADFGLAKLAEEGTGTGIVGTFGYMPPEYALYGEVSPKLDVYAFGVVLFEIISGRVAISSALPSENDQQSPAQNRESRTLTSFFEPVLNDPDGKTLLPKCIDPALNGEYSLDAVWKMAQLARRCTHQSPDMRPTMRFAVVQLMTLASVTQEWDVGYFSRASSQSQPPSGNDQL | ||||||
Disulfide bond | 43↔104 | |||||
Sequence: CIPPNGCKALAYYRLKQGDDLEKLQGRFQTNNSEVLAYNPQLVDANSIQAGTNIYLPFDCLC | ||||||
Disulfide bond | 49↔166 | |||||
Sequence: CKALAYYRLKQGDDLEKLQGRFQTNNSEVLAYNPQLVDANSIQAGTNIYLPFDCLCLNGELVHRFSYTVTTNDTAEKVVDVTYQKLTTVGAVRSASNSGDLSSIYSGQSLTIPVRCYC | ||||||
Disulfide bond | 102↔164 | |||||
Sequence: CLCLNGELVHRFSYTVTTNDTAEKVVDVTYQKLTTVGAVRSASNSGDLSSIYSGQSLTIPVRC |
Keywords
- PTM
Proteomic databases
Expression
Induction
Up-regulated in response to chitosan.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 53-98 | LysM 1 | ||||
Sequence: AYYRLKQGDDLEKLQGRFQTNNSEVLAYNPQLVDANSIQAGTNIYL | ||||||
Domain | 113-160 | LysM 2 | ||||
Sequence: FSYTVTTNDTAEKVVDVTYQKLTTVGAVRSASNSGDLSSIYSGQSLTI | ||||||
Domain | 179-227 | LysM 3 | ||||
Sequence: STYVVQADDQLTSLSTNFSVDADVISKFNSDTRNLSPDSIIFIPSKAAN | ||||||
Region | 286-308 | Disordered | ||||
Sequence: MQQSGLLSSSSMAGSKPSRSGST | ||||||
Domain | 330-612 | Protein kinase | ||||
Sequence: FSLAKKIGQGGFASVYYGVIRDQKLAIKKMTLQCTKEFLAELQVLTNVHHTNLVQLIGYCTTNSLFLVYEYIENGTLDHHLRRRKSDDKPPLSWLQRVQICLDSARGLEYIHEHTKPTYIHRDIKSANILLDDNFRAKVADFGLAKLAEEGTGTGIVGTFGYMPPEYALYGEVSPKLDVYAFGVVLFEIISGRVAISSALPSENDQQSPAQNRESRTLTSFFEPVLNDPDGKTLLPKCIDPALNGEYSLDAVWKMAQLARRCTHQSPDMRPTMRFAVVQLMTL |
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length640
- Mass (Da)70,884
- Last updated2018-02-28 v2
- Checksum9D7C2A7B4D034D22
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 236-238 | in Ref. 1; no nucleotide entry | ||||
Sequence: YVL → SSG | ||||||
Sequence conflict | 240-243 | in Ref. 1; no nucleotide entry | ||||
Sequence: TVHW → GSS |
Keywords
- Technical term