A9T6G5 · TRM5_PHYPA

Function

function

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.

Catalytic activity

Features

Showing features for binding site.

148050100150200250300350400450
TypeIDPosition(s)Description
Binding site215S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site253-254S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site281-282S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site371S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentmitochondrial matrix
Cellular Componentnucleus
Molecular FunctiontRNA (guanine(37)-N1)-methyltransferase activity
Molecular FunctiontRNA methyltransferase activity
Biological ProcesstRNA N1-guanine methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA (guanine(37)-N1)-methyltransferase
  • EC number
  • Alternative names
    • M1G-methyltransferase
    • tRNA [GM37] methyltransferase
    • tRNA methyltransferase 5 homolog

Gene names

    • ORF names
      PHYPADRAFT_141055

Organism names

Accessions

  • Primary accession
    A9T6G5

Proteomes

Subcellular Location

Mitochondrion matrix
Nucleus
Cytoplasm
Note: Predominantly in the mitochondria and in the nucleus.

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004141381-480tRNA (guanine37-N1)-methyltransferase

Proteomic databases

Interaction

Subunit

Monomer.

Structure

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias312-332Polar residues
Region312-346Disordered

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    480
  • Mass (Da)
    53,604
  • Last updated
    2008-02-05 v1
  • Checksum
    F10B0D2F7BACE76D
MAAPGCSPVGIDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAIAQPDLEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEILPAGCEVPSSFETIGHIAHLNLREDLLTYKKIIAEVILDKNPKLKTVVNKVGTITNEFRVPEFEILAGEPSLVTEIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIGPFAIPAAQQGCAVYANDLNPTSVKFLKLNSDINKVGESIKAFNLDAREFMRKLVTEEEGCVGEIASSTLIAENSLNGTRIPSSKENSSSLTEGSDMPSSKKPEERKAKKAKVSEVVKMKISDVKPWEHFDHVVMNLPASALEFLDVLNGLLCKGRWKGTMPRVHCYCFMRSNETNADIVKKAETYLGGSIADPDVYVVRDVAPNKASLAFTAVVSWFISRSFIWSLYFFCLIQAHLVLDSIEHR

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias312-332Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DS545062
EMBL· GenBank· DDBJ
EDQ60996.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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