A9T6G5 · TRM5_PHYPA
- ProteintRNA (guanine(37)-N1)-methyltransferase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids480 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Catalytic activity
- guanosine37 in tRNA + S-adenosyl-L-methionine = H+ + N1-methylguanosine37 in tRNA + S-adenosyl-L-homocysteine
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 215 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 253-254 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DL | ||||||
Binding site | 281-282 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DA | ||||||
Binding site | 371 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | mitochondrial matrix | |
Cellular Component | nucleus | |
Molecular Function | tRNA (guanine(37)-N1)-methyltransferase activity | |
Molecular Function | tRNA methyltransferase activity | |
Biological Process | tRNA N1-guanine methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (guanine(37)-N1)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Bryophyta > Bryophytina > Bryopsida > Funariidae > Funariales > Funariaceae > Physcomitrium
Accessions
- Primary accessionA9T6G5
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Predominantly in the mitochondria and in the nucleus.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000414138 | 1-480 | tRNA (guanine37-N1)-methyltransferase | |||
Sequence: MAAPGCSPVGIDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAIAQPDLEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEILPAGCEVPSSFETIGHIAHLNLREDLLTYKKIIAEVILDKNPKLKTVVNKVGTITNEFRVPEFEILAGEPSLVTEIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIGPFAIPAAQQGCAVYANDLNPTSVKFLKLNSDINKVGESIKAFNLDAREFMRKLVTEEEGCVGEIASSTLIAENSLNGTRIPSSKENSSSLTEGSDMPSSKKPEERKAKKAKVSEVVKMKISDVKPWEHFDHVVMNLPASALEFLDVLNGLLCKGRWKGTMPRVHCYCFMRSNETNADIVKKAETYLGGSIADPDVYVVRDVAPNKASLAFTAVVSWFISRSFIWSLYFFCLIQAHLVLDSIEHR |
Proteomic databases
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 312-332 | Polar residues | ||||
Sequence: NGTRIPSSKENSSSLTEGSDM | ||||||
Region | 312-346 | Disordered | ||||
Sequence: NGTRIPSSKENSSSLTEGSDMPSSKKPEERKAKKA |
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length480
- Mass (Da)53,604
- Last updated2008-02-05 v1
- ChecksumF10B0D2F7BACE76D
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 312-332 | Polar residues | ||||
Sequence: NGTRIPSSKENSSSLTEGSDM |
Keywords
- Technical term