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A9QPH3 · A9QPH3_METI4

Function

function

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.

Catalytic activity

Features

Showing features for binding site, active site, site.

134750100150200250300
Type
IDPosition(s)Description
Binding site11-12NAD+ (UniProtKB | ChEBI)
Binding site36NAD+ (UniProtKB | ChEBI)
Binding site131NAD+ (UniProtKB | ChEBI)
Binding site162-164D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Active site163Nucleophile
Site191Activates thiol group during catalysis
Binding site194D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site223-224D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site246D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site328NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Molecular FunctionNAD binding
Molecular FunctionNADP binding
Biological Processglucose metabolic process
Biological Processglycolytic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glyceraldehyde-3-phosphate dehydrogenase
  • EC number

Gene names

    • Name
      gapA
    • Ordered locus names
      Minf_1033

Organism names

Accessions

  • Primary accession
    A9QPH3

Proteomes

Subcellular Location

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-163Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    347
  • Mass (Da)
    37,472
  • Last updated
    2008-02-05 v1
  • MD5 Checksum
    5338F934519E2F6ABA87BD47EED31A00
MPKVAINGFGRIGRLILRAMAEQGLLKELPVVAVNDIVSADNLAYLLKYDTNYGRIPVEVKSAKSKDSLEEDDLLIVDGQTIQCLAIKEGPAAMPWKELGVDIVFESTGLFTAANKALGHIKSGAKKVIITAPGKDADITVVMGVNHTELDLAKHSIISNASCTTNCLTPLVHVLLKEGLGIEEGLMTTVHSYTASQRLQDGPSKKDWKGGRAAEGNIIPATTGAAKATALVIPELKGKLTGMSFRVPTPTVSVVDLTVRTEKETSYSEICGLMKKASESYLSGILGYTDEQVVSRDFLKDSHSSIFDASSGIGLNSRFYKLIAWYDNEWGYSCRCVDLARYVASRL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EU223889
EMBL· GenBank· DDBJ
ABX56631.1
EMBL· GenBank· DDBJ
Genomic DNA
CP000975
EMBL· GenBank· DDBJ
ACD83088.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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