A9QPH3 · A9QPH3_METI4
- ProteinGlyceraldehyde-3-phosphate dehydrogenase
- GenegapA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids347 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.
Catalytic activity
- D-glyceraldehyde 3-phosphate + phosphate + NAD+ = (2R)-3-phospho-glyceroyl phosphate + NADH + H+
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 11-12 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 36 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 131 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 162-164 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Active site | 163 | Nucleophile | |||
Site | 191 | Activates thiol group during catalysis | |||
Binding site | 194 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 223-224 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 246 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 328 | NAD+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity | |
Molecular Function | NAD binding | |
Molecular Function | NADP binding | |
Biological Process | glucose metabolic process | |
Biological Process | glycolytic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlyceraldehyde-3-phosphate dehydrogenase
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Verrucomicrobiota > Methylacidiphilae > Methylacidiphilales > Methylacidiphilaceae > Methylacidiphilum (ex Ratnadevi et al. 2023)
Accessions
- Primary accessionA9QPH3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 2-163 | Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding | |||
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length347
- Mass (Da)37,472
- Last updated2008-02-05 v1
- MD5 Checksum5338F934519E2F6ABA87BD47EED31A00
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
EU223889 EMBL· GenBank· DDBJ | ABX56631.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP000975 EMBL· GenBank· DDBJ | ACD83088.1 EMBL· GenBank· DDBJ | Genomic DNA |