A9CH39 · PHRB_AGRFC
- Protein(6-4) photolyase
- GenephrB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids507 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Photolyase involved in the repair of UV-induced (6-4) lesions in DNA. Catalyzes the photoreactivation of (6-4) pyrimidine-pyrimidone photoproducts by using blue-light energy. Can repair (6-4) photoproducts in ssDNA as well as in dsDNA.
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 FAD per subunit.
Note: Binds 6,7-dimethyl-8-ribityllumazine (DMRL) as antenna chromophore.
Note: Binds 1 [4Fe-4S] cluster per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 9-10 | 6,7-dimethyl-8-(1-D-ribityl)lumazine (UniProtKB | ChEBI) | |||
Binding site | 32-40 | 6,7-dimethyl-8-(1-D-ribityl)lumazine (UniProtKB | ChEBI) | |||
Binding site | 105 | 6,7-dimethyl-8-(1-D-ribityl)lumazine (UniProtKB | ChEBI) | |||
Binding site | 265-269 | FAD (UniProtKB | ChEBI) | |||
Binding site | 273 | FAD (UniProtKB | ChEBI) | |||
Binding site | 350 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 363-366 | FAD (UniProtKB | ChEBI) | |||
Binding site | 397 | FAD (UniProtKB | ChEBI) | |||
Binding site | 406 | FAD (UniProtKB | ChEBI) | |||
Binding site | 438 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 441 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 454 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | DNA (6-4) photolyase activity | |
Molecular Function | DNA binding | |
Molecular Function | FAD binding | |
Molecular Function | metal ion binding | |
Biological Process | photoreactive repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name(6-4) photolyase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Rhizobiaceae > Rhizobium/Agrobacterium group > Agrobacterium > Agrobacterium tumefaciens complex
Accessions
- Primary accessionA9CH39
Proteomes
Phenotypes & Variants
Disruption phenotype
Cells lacking this gene show a strongly attenuated photoreactivation after treatment with UV-B irradiation.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000431763 | 1-507 | (6-4) photolyase | ||
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length507
- Mass (Da)57,864
- Last updated2008-01-15 v1
- Checksum9DB788BDA687ECF5
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE007870 EMBL· GenBank· DDBJ | AAK88685.1 EMBL· GenBank· DDBJ | Genomic DNA |