A9CH39 · PHRB_AGRFC

Function

function

Photolyase involved in the repair of UV-induced (6-4) lesions in DNA. Catalyzes the photoreactivation of (6-4) pyrimidine-pyrimidone photoproducts by using blue-light energy. Can repair (6-4) photoproducts in ssDNA as well as in dsDNA.

Catalytic activity

  • (6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA).
    EC:4.1.99.13 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 1 FAD per subunit.
6,7-dimethyl-8-(1-D-ribityl)lumazine (UniProtKB | Rhea| CHEBI:58201 )

Note: Binds 6,7-dimethyl-8-ribityllumazine (DMRL) as antenna chromophore.
[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site9-106,7-dimethyl-8-(1-D-ribityl)lumazine (UniProtKB | ChEBI)
Binding site32-406,7-dimethyl-8-(1-D-ribityl)lumazine (UniProtKB | ChEBI)
Binding site1056,7-dimethyl-8-(1-D-ribityl)lumazine (UniProtKB | ChEBI)
Binding site265-269FAD (UniProtKB | ChEBI)
Binding site273FAD (UniProtKB | ChEBI)
Binding site350[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site363-366FAD (UniProtKB | ChEBI)
Binding site397FAD (UniProtKB | ChEBI)
Binding site406FAD (UniProtKB | ChEBI)
Binding site438[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site441[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site454[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular FunctionDNA (6-4) photolyase activity
Molecular FunctionDNA binding
Molecular FunctionFAD binding
Molecular Functionmetal ion binding
Biological Processphotoreactive repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    (6-4) photolyase
  • EC number
  • Alternative names
    • (6-4)DNA photolyase
    • DNA photolyase PhrB
    • Photoreactivating enzyme PhrB

Gene names

    • Name
      phrB
    • Ordered locus names
      Atu4765

Organism names

Accessions

  • Primary accession
    A9CH39

Proteomes

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene show a strongly attenuated photoreactivation after treatment with UV-B irradiation.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004317631-507(6-4) photolyase

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    507
  • Mass (Da)
    57,864
  • Last updated
    2008-01-15 v1
  • Checksum
    9DB788BDA687ECF5
MSQLVLILGDQLSPSIAALDGVDKKQDTIVLCEVMAEASYVGHHKKKIAFIFSAMRHFAEELRGEGYRVRYTRIDDADNAGSFTGEVKRAIDDLTPSRICVTEPGEWRVRSEMDGFAGAFGIQVDIRSDRRFLSSHGEFRNWAAGRKSLTMEYFYREMRRKTGLLMNGEQPVGGRWNFDAENRQPARPDLLRPKHPVFAPDKITKEVIDTVERLFPDNFGKLENFGFAVTRTDAERALSAFIDDFLCNFGATQDAMLQDDPNLNHSLLSFYINCGLLDALDVCKAAERAYHEGGAPLNAVEGFIRQIIGWREYMRGIYWLAGPDYVDSNFFENDRSLPVFYWTGKTHMNCMAKVITETIENAYAHHIQRLMITGNFALLAGIDPKAVHRWYLEVYADAYEWVELPNVIGMSQFADGGFLGTKPYAASGNYINRMSDYCDTCRYDPKERLGDNACPFNALYWDFLARNREKLKSNHRLAQPYATWARMSEDVRHDLRAKAAAFLRKLD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE007870
EMBL· GenBank· DDBJ
AAK88685.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help