A8WGB1 · CCBE1_DANRE

Function

function

Required for lymphangioblast budding and angiogenic sprouting from venous endothelium during embryogenesis. Required for the formation of facial lymphatic structures (PubMed:37097004).
Necessary for lymphangiogenesis, but is probably not part of either the vegfc-vegfr3 signaling or sox18-prox1 transcriptional pathways

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcollagen trimer
Cellular Componentextracellular region
Molecular Functioncalcium ion binding
Biological Processblood vessel morphogenesis
Biological Processlymph vessel development
Biological Processlymphangiogenesis
Biological Processsprouting angiogenesis
Biological Processvascular endothelial growth factor signaling pathway
Biological Processvenous blood vessel morphogenesis
Biological Processvenous endothelial cell migration involved in lymph vessel development

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Collagen and calcium-binding EGF domain-containing protein 1
  • Alternative names
    • Full of fluid protein

Gene names

    • Name
      ccbe1
    • Synonyms
      fof

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio

Accessions

  • Primary accession
    A8WGB1

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Significant reduction in phosphorylated mapk/erk in the nuclei within the posterior cardinal vein endothelium at 32 hpf (PubMed:28179432).
Loss of facial lymphatic vessels including; lateral facial lymphatic vessel, medial facial lymphatic vessel, facial lymphatic branchial arches and otolithic lymphatic vessel at 5 dpf (PubMed:37097004).
In a svep1 and ccbe1 double knockout model there is complete loss of the facial lymphatic architecture at 5 dpf (PubMed:37097004).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis162In fof(hu3613); lacks the thoracic duct and the previously unidentified intersegmental (ISLVs) and dorsal longitudinal lymphatic vessels (DLLVs) but retains a seemingly normal blood vasculature. Mutants show edema, initiating in the lower intestine and around the eyes from 6 dpf. At 36 dpf, surviving mutants show severe edema.

PTM/Processing

Features

Showing features for signal, chain, disulfide bond, glycosylation.

TypeIDPosition(s)Description
Signal1-22
ChainPRO_000037063623-401Collagen and calcium-binding EGF domain-containing protein 1
Disulfide bond130↔142
Glycosylation134N-linked (GlcNAc...) asparagine
Disulfide bond138↔151
Disulfide bond153↔166

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Not expressed in blood or lymphatic endothelial cells, correlating spatially and temporally with the migration routes of endothelial cells that bud from the PCV, migrate in association with somite boundaries and seed the horizontal myoseptum region from where lymphatic precursors later migrate.

Developmental stage

Restricted expression during development with expression in the pronephros and ventral mesenchyme at 24 hours post fertilization (hpf). By 36 hpf, expression is detected in discrete zones along each somitic boundary, between the PCV and the horizontal myoseptum, as well as along the horizontal myoseptum itself. At 48 hpf, expression is restricted along the horizontal myoseptum.

Gene expression databases

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain126-167EGF-like; calcium-binding
Region229-321Disordered
Domain234-276Collagen-like 1
Domain286-319Collagen-like 2
Region344-401Disordered

Sequence similarities

Belongs to the CCBE1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    401
  • Mass (Da)
    43,849
  • Last updated
    2009-05-05 v2
  • Checksum
    27F2BB0FF261DCBC
MIYPGRGASLSVAVALVLFSSGAPWTFREEKEDVDREVCSESKIATTKYPCVKSTGEVTTCYRKKCCEGFKFVLGQCIPEDYDVCAGAPCEQQCTDHFGRVVCTCYDGYRYDRERHRNREKPYCLDIDECANNNETVCSQMCVNTPGSYRCDCHSGFYLEDDGKTCTKGERAPLFEKSDNVMKEGTCSATCEDFHQMKMTVLQLKQKMSLLSSNTEINKQMTNEKMMMTTNSFLPGPPGPPGPAGTPGAKGSSGSPGQMGPPGLPGPRGDMGPIGPSPDLSHIKQGRRGPVGPPGAPGRDGMKGERGFPGPSGPPGPPGSFDFLLLMMADIRNDIAELQSKVFSRPLHSSFEDFPSAPDSWRDTPENLDFGSGEDYKSQSPPKSSRKRKLPRNLKNPDWPV

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8M6YWX1A0A8M6YWX1_DANREccbe1328
A0A8M9P3B9A0A8M9P3B9_DANREccbe1359

Sequence caution

The sequence AAI54644.1 differs from that shown. Reason: Erroneous initiation

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BC154643
EMBL· GenBank· DDBJ
AAI54644.1
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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