A8TGA1 · XYS20_NEOPA
- ProteinEndo-1,4-beta-xylanase S20
- GenexynS20
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids335 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.
Catalytic activity
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
4.9 mg/ml | oat spelt xylan |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
3.886 μmol/min/mg | toward oat spelt xylan |
pH Dependence
Optimum pH is 6.0.
Temperature Dependence
Optimum temperature is 45 degrees Celsius.
Pathway
Glycan degradation; xylan degradation.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 134 | Nucleophile | ||||
Sequence: E | ||||||
Active site | 228 | Proton donor | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | cellulose binding | |
Molecular Function | endo-1,4-beta-xylanase activity | |
Biological Process | xylan catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameEndo-1,4-beta-xylanase S20
- EC number
- Short namesXylanase S20
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Fungi incertae sedis > Chytridiomycota > Chytridiomycota incertae sedis > Neocallimastigomycetes > Neocallimastigales > Neocallimastigaceae > Neocallimastix
Accessions
- Primary accessionA8TGA1
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-22 | |||||
Sequence: MLRKLVTGALAAALLLSGQSNA | ||||||
Chain | PRO_0000429664 | 23-335 | Endo-1,4-beta-xylanase S20 | |||
Sequence: QNACQQTQQLSGGRTINNKNETGNGNGNYKYEIWRDGNGGSLTLYPKDAAFKASWNNSGDFLGRVGLTFNKPAATNLGGDLIANYNYKKSGSDGGTYSYIGIYGWMDNPQIEYYVVDDWMHNRGAPGGSYMGSQKGTITVDGGTYKVWSGQRTGASKWGTSTFTQIFSIRTSPRQCGSINVSEHFRQWQKLGLRLGGLMEAQLLAESGGGSGYVDFTYATITIGGSSSNASAPSNNNNNNNNNNDNNGNWNNWNNNNNNNNNNNNNNNNNNNNQGGGNCAAIWGQCGGSGYNGPKCCKQGSCKQINQWYSQCQ | ||||||
Glycosylation | 42 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 78 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 202 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 251 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 39-241 | GH11 | ||||
Sequence: NNKNETGNGNGNYKYEIWRDGNGGSLTLYPKDAAFKASWNNSGDFLGRVGLTFNKPAATNLGGDLIANYNYKKSGSDGGTYSYIGIYGWMDNPQIEYYVVDDWMHNRGAPGGSYMGSQKGTITVDGGTYKVWSGQRTGASKWGTSTFTQIFSIRTSPRQCGSINVSEHFRQWQKLGLRLGGLMEAQLLAESGGGSGYVDFTYA | ||||||
Region | 251-291 | Disordered | ||||
Sequence: NASAPSNNNNNNNNNNDNNGNWNNWNNNNNNNNNNNNNNNN | ||||||
Domain | 300-335 | CBM1 | ||||
Sequence: NCAAIWGQCGGSGYNGPKCCKQGSCKQINQWYSQCQ |
Sequence similarities
Belongs to the glycosyl hydrolase 11 (cellulase G) family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length335
- Mass (Da)36,094
- Last updated2008-01-15 v1
- Checksum5F2B450DA1CA8D34