A8NCK4 · COX1_COPC7

Function

function

Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of alpha-cuprenene and oxidized derivatives (PubMed:19400802).
The alpha-cuprenene synthase COP6 is the only sesquiterpene synthase identified in C.cinereus that appears to be part of a biosynthetic gene cluster and is highly specific since it catalyzes the cyclization of (2E,6E)-farnesyl diphosphate into only one product, alpha-cuprenene (PubMed:19400802, PubMed:20419721).
The cytochrome P450 monooxygenase COX2 then oxidizes the cyclohexadiene ring of alpha-cuprenene at positions 1 and 4, yielding first alpha-cuparene, followed by alpha-cuparophenol and a further yet unidentified compound resulting from one additional oxidation step (PubMed:19400802).
The cytochrome P450 monooxygenase COX1 then likely catalyzes the oxidation at position 9 of the pentane ring of alpha-cuprenene to give the corresponding hydroxy or ketone derivatives (PubMed:19400802).

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site450Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome P450 monooxygenase COX1
  • EC number
  • Alternative names
    • Alpha-cuprenene oxidase 1

Gene names

    • Name
      COX1
    • ORF names
      CC1G_03562

Organism names

Accessions

  • Primary accession
    A8NCK4

Proteomes

Organism-specific databases

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane7-25Helical

Keywords

PTM/Processing

Features

Showing features for chain, glycosylation.

Type
IDPosition(s)Description
ChainPRO_00004446371-518Cytochrome P450 monooxygenase COX1
Glycosylation48N-linked (GlcNAc...) asparagine
Glycosylation100N-linked (GlcNAc...) asparagine
Glycosylation292N-linked (GlcNAc...) asparagine
Glycosylation302N-linked (GlcNAc...) asparagine
Glycosylation351N-linked (GlcNAc...) asparagine
Glycosylation457N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    518
  • Mass (Da)
    58,438
  • Last updated
    2010-08-10 v2
  • MD5 Checksum
    E8022F80892C182515D73207C77D1355
MTSTTQVLIALSSIVVAYFVKTALAKRKLNPRGLPYPPGPKGLPVIGNLTQLPQHKPWLVYKEWGRTYGDLMYLEAMGQPMIIINSLSRALDLLDKRAVNYSDRPHVPTMDLCVLMKLDWVFAFMQYGADWRNHRRAFHQYLNHNMVHNYHPIQEQETQEFLRALIARPKDFLAHTRHLFGSIIIRISYGFEDEEYNKVLVEEAEALASGFSESIIPGRYLVNAFPFLRHVPSWLPGAGFQRTMQYLRKISEKTLSEPFDNVKEALKTGNRQVGPSLAVGLIESLPDETHANRTSLEVVARNTSALSYIAGADTTVSSAQALILALAMHPEVQRKAQKEIDSVVGTDRLPNMSDKPNMPYVQAIVKEAGRWHTVLPLGFIHVSAKEDEYDGYFIPKGSFIFVNTWAIMHDPDVFKNPLQFNPERYLKNGQIDTSVLDPEAATFGFGRRICPGRWLSNDSLFLMAASLLATFNIAAPKDRTGKPIPLSLDTSSHLITAPLPYDCEFQLRSPKYAALLQK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AACS02000009
EMBL· GenBank· DDBJ
EAU89297.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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