A8HYJ1 · TOC34_CHLRE
- ProteinTranslocase of chloroplast 34 homolog, chloroplastic
- GeneTOC34
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids397 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis (By similarity).
Functions as an essential component of the outer chloroplast membrane translocon (TOC) complex, which, in turn, catalyzes the import of nucleus-encoded precursor polypeptides from the cytoplasm to the chloroplast (PubMed:23167510).
Functions as an essential component of the outer chloroplast membrane translocon (TOC) complex, which, in turn, catalyzes the import of nucleus-encoded precursor polypeptides from the cytoplasm to the chloroplast (PubMed:23167510).
Cofactor
Note: Binds 1 Mg2+ ion by subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast outer membrane | |
Molecular Function | GTP binding | |
Molecular Function | hydrolase activity | |
Molecular Function | metal ion binding | |
Biological Process | protein transport |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTranslocase of chloroplast 34 homolog, chloroplastic
- EC number
- Short namesCr-TOC34 ; TOC34p
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Chlorophyta > core chlorophytes > Chlorophyceae > CS clade > Chlamydomonadales > Chlamydomonadaceae > Chlamydomonas
Accessions
- Primary accessionA8HYJ1
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Plastid, chloroplast outer membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 329-349 | Helical | ||||
Sequence: LLPVAIAAEVLFYRRFLHPRL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000442532 | 1-397 | Translocase of chloroplast 34 homolog, chloroplastic | |||
Sequence: MAQPPRPAEEYDDDVQEDEDELKEGELDDDESHEAASEGGEAAAGDEEAEDDEQDEEDGDEDSQPWAGLNRLPERDDMLDILNELRAEGRKQLTVLLLGKSSVGKSSLINSLLGEAVVRVQAFKLQADTDITTTVVRQVAVGNSEVDGFRLKLIDTCGLEDPEAGDTVNLGALSKIAEDVRGVGIDVVLYCDRLDLYRVDPLDKAIIDAISSTFGRGIWRRTVVALTHANLVQTPPGTDYDSFVNGRVRLIRGAVRGPLFFRPSLPVALVENSETCPVSSESGFRVLPDGEPWLVALVSQLVDMAAARRRPYKYHPRLSSKPSHRFRWLLPVAIAAEVLFYRRFLHPRLDDNQRRVEREEERVWALRGQQRRALGLHRPHRPDKDAAWRLEQMYDDD |
Proteomic databases
Interaction
Subunit
Homodimer, heterodimer with other TOC proteins, and monomer. Part of the TOC core complex that includes 1 protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP-binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex (By similarity).
Interacts with ARSA1 (PubMed:28382961).
Interacts with ARSA1 (PubMed:28382961).
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-72 | Disordered | ||||
Sequence: MAQPPRPAEEYDDDVQEDEDELKEGELDDDESHEAASEGGEAAAGDEEAEDDEQDEEDGDEDSQPWAGLNRL | ||||||
Compositional bias | 11-63 | Acidic residues | ||||
Sequence: YDDDVQEDEDELKEGELDDDESHEAASEGGEAAAGDEEAEDDEQDEEDGDEDS | ||||||
Domain | 90-321 | AIG1-type G | ||||
Sequence: RKQLTVLLLGKSSVGKSSLINSLLGEAVVRVQAFKLQADTDITTTVVRQVAVGNSEVDGFRLKLIDTCGLEDPEAGDTVNLGALSKIAEDVRGVGIDVVLYCDRLDLYRVDPLDKAIIDAISSTFGRGIWRRTVVALTHANLVQTPPGTDYDSFVNGRVRLIRGAVRGPLFFRPSLPVALVENSETCPVSSESGFRVLPDGEPWLVALVSQLVDMAAARRRPYKYHPRLSSK | ||||||
Region | 99-106 | G1 | ||||
Sequence: GKSSVGKS | ||||||
Region | 121-124 | Homodimerization | ||||
Sequence: QAFK | ||||||
Region | 126-130 | G2 | ||||
Sequence: QADTD | ||||||
Region | 155-158 | G3 | ||||
Sequence: DTCG | ||||||
Region | 193-198 | Homodimerization | ||||
Sequence: RLDLYR | ||||||
Region | 227-230 | G4 | ||||
Sequence: THAN | ||||||
Region | 271-273 | G5 | ||||
Sequence: ENS | ||||||
Motif | 350-358 | AKR2A-binding sequence (ABS) required for chloroplast outer envelope membrane targeting | ||||
Sequence: DDNQRRVER |
Sequence similarities
Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. TOC34 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length397
- Mass (Da)44,542
- Last updated2007-12-04 v1
- Checksum2A4AD399A5939A3C
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 11-63 | Acidic residues | ||||
Sequence: YDDDVQEDEDELKEGELDDDESHEAASEGGEAAAGDEEAEDDEQDEEDGDEDS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GU814014 EMBL· GenBank· DDBJ | ADF43133.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DS496110 EMBL· GenBank· DDBJ | EDP08621.1 EMBL· GenBank· DDBJ | Genomic DNA |