A8H1B4 · TYSY_SHEPA

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Catalytic activity

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

128320406080100120140160180200220240260280
TypeIDPosition(s)Description
Binding site22dUMP (UniProtKB | ChEBI)
Active site160Nucleophile
Binding site180-183dUMP (UniProtKB | ChEBI)
Binding site183(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site191dUMP (UniProtKB | ChEBI)
Binding site221-223dUMP (UniProtKB | ChEBI)
Binding site282(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thymidylate synthase
  • EC number
  • Short names
    TS
    ; TSase

Gene names

    • Name
      thyA
    • Ordered locus names
      Spea_1024

Organism names

Accessions

  • Primary accession
    A8H1B4

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000738851-283Thymidylate synthase

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    283
  • Mass (Da)
    32,211
  • Last updated
    2007-11-13 v1
  • Checksum
    0F3F09D941B5F6F7
MKQYLDLCQRLIDEGTWVENSRTGKRCLTVINADLVYHVDKNEFPLITTRKSFYKAAIAELLGYIRGYDNAADFRAIGCNTWNANANDNQAWLDNPHRKGIDDMGRVYGVQGRAWSKPDGGCIDQLRKIVDDLSKGIDDRGEILSFYNPGEFHMGCLRPCMHTHNFSLVGDTLHLTSFQRSCDVPLGLNFNQVQVFTLLALMAQITGHKPGTAFHKIVNAHIYEDQLELMQEVQLKREPFTSPKLVINPNIKSLEDLETWVSIDDFEVTGYEHHEAISYPFSV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000851
EMBL· GenBank· DDBJ
ABV86351.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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