A8H1B4 · TYSY_SHEPA
- ProteinThymidylate synthase
- GenethyA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids283 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.
Catalytic activity
- dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP
Pathway
Pyrimidine metabolism; dTTP biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 22 | dUMP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Active site | 160 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 180-183 | dUMP (UniProtKB | ChEBI) | ||||
Sequence: RSCD | ||||||
Binding site | 183 | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 191 | dUMP (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 221-223 | dUMP (UniProtKB | ChEBI) | ||||
Sequence: HIY | ||||||
Binding site | 282 | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | thymidylate synthase activity | |
Biological Process | dTMP biosynthetic process | |
Biological Process | dTTP biosynthetic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThymidylate synthase
- EC number
- Short namesTS ; TSase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Alteromonadales > Shewanellaceae > Shewanella
Accessions
- Primary accessionA8H1B4
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000073885 | 1-283 | Thymidylate synthase | |||
Sequence: MKQYLDLCQRLIDEGTWVENSRTGKRCLTVINADLVYHVDKNEFPLITTRKSFYKAAIAELLGYIRGYDNAADFRAIGCNTWNANANDNQAWLDNPHRKGIDDMGRVYGVQGRAWSKPDGGCIDQLRKIVDDLSKGIDDRGEILSFYNPGEFHMGCLRPCMHTHNFSLVGDTLHLTSFQRSCDVPLGLNFNQVQVFTLLALMAQITGHKPGTAFHKIVNAHIYEDQLELMQEVQLKREPFTSPKLVINPNIKSLEDLETWVSIDDFEVTGYEHHEAISYPFSV |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length283
- Mass (Da)32,211
- Last updated2007-11-13 v1
- Checksum0F3F09D941B5F6F7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000851 EMBL· GenBank· DDBJ | ABV86351.1 EMBL· GenBank· DDBJ | Genomic DNA |