A8GG79 · TRI1_SERP5

Function

function

Immunity component of a contact-dependent interbacterial competition system (also called effector-immunity systems). Acts as an arginine mono-ADP-ribosylhydrolase, mediating the removal of mono-ADP-ribose attached to arginine residues on proteins. De-ADP-ribosylates FtsZ, is able to act on other proteins as well. Neutralizes the toxic activity of cognate toxin Tre1-Sp. Expression of this protein alone in E.coli partially protects the cells against competition by wild-type S.proteamaculans. Neutralizes Tre1-Sp both by occluding its active site via its N-terminal extension and by hydrolyzing the ADP-ribosyl moiety from FtsZ; the 2 activities are dissociable by mutagenesis.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site116Mg2+ (UniProtKB | ChEBI)
Binding site117Mg2+ (UniProtKB | ChEBI)
Binding site118Mg2+ (UniProtKB | ChEBI)
Binding site161Mg2+ (UniProtKB | ChEBI)
Binding site317Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionADP-ribosylarginine hydrolase activity
Molecular Functionmetal ion binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ADP-ribosylarginine hydrolase Tri1
  • EC number
  • Alternative names
    • Immunity protein Tri1
      (Tri1-Sp
      )

Gene names

    • Name
      tri1
    • Ordered locus names
      Spro_3018

Organism names

Accessions

  • Primary accession
    A8GG79

Phenotypes & Variants

Disruption phenotype

A double tre1-tri1 deletion is outcompeted by wild-type cells, but not by wild-type cells missing a type 6 secretion system (T6SS).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis32Partially protects E.coli against cognate toxin Tre1. No longer protects E.coli; when associated with N-161.
Mutagenesis161Partially protects E.coli against cognate toxin Tre1, no longer de-ADP-ribosylates FtsZ. No longer protects E.coli; when associated with E-32.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004465171-366ADP-ribosylarginine hydrolase Tri1

Interaction

Subunit

Forms a stable complex with cognate effector protein Tre1-Sp.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-65N-terminal extension
Region74-366ADP-ribosyl hydrolase domain

Domain

The N-terminal extension forms a projection that reaches into the active site of cognate toxin Tre1-Sp where it occludes the active site.

Sequence similarities

Belongs to the ADP-ribosylglycohydrolase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    366
  • Mass (Da)
    40,934
  • Last updated
    2007-11-13 v1
  • Checksum
    A9443B5B3280CC54
MIDLREDTWTLQLYAQRYKGLSPKNSRELQLRMEYDPLKPNLPTSGEEQNSKPEWLNTPPCLIPESESLDKAKGALVGLAIGDAIGTTLEFLPRDKLHVNDMVGGGPFRLQPGEWTDDTSMALCLAESYISAGRLDITLFREKLVRWYRHGENSSNGRCFDIGNTTRNALEQYLKHGASWFGNTEPETAGNAAIIRQAPTSIFRRKSLQRTFADSDSQSMATHCAPESMASCQFLGFILNYLINGSSREKAFSPHVMPLPVRVLLINAGEYKEKKRDEIRSSGYVIDTLEAAMWAVWNTDNFHDAILLAANLGDDADSVAATTGQIAGALYGYSNIPKPWLDKLVQQERISNLAEQLFYMAPEEDF

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000826
EMBL· GenBank· DDBJ
ABV42119.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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