A8FCG8 · MTNW_BACP2
- Protein2,3-diketo-5-methylthiopentyl-1-phosphate enolase
- GenemtnW
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids406 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P).
Miscellaneous
Has no RuBP-carboxylation activity.
Catalytic activity
- 5-methylsulfanyl-2,3-dioxopentyl phosphate = 2-hydroxy-5-methylsulfanyl-3-oxopent-1-enyl phosphate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 94 | Proton acceptor | ||||
Sequence: K | ||||||
Binding site | 143 | substrate | ||||
Sequence: K | ||||||
Binding site | 169 | Mg2+ (UniProtKB | ChEBI); via carbamate group | ||||
Sequence: K | ||||||
Binding site | 169-172 | substrate | ||||
Sequence: KDDE | ||||||
Binding site | 171 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 172 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 260 | substrate | ||||
Sequence: H | ||||||
Binding site | 332 | substrate | ||||
Sequence: G | ||||||
Binding site | 354-355 | substrate | ||||
Sequence: GG |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | ribulose-bisphosphate carboxylase activity | |
Biological Process | carbon fixation | |
Biological Process | L-methionine salvage from methylthioadenosine | |
Biological Process | L-methionine salvage from S-adenosylmethionine |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2,3-diketo-5-methylthiopentyl-1-phosphate enolase
- EC number
- Short namesDK-MTP-1-P enolase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionA8FCG8
Proteomes
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000357286 | 1-406 | 2,3-diketo-5-methylthiopentyl-1-phosphate enolase | |||
Sequence: MSELLATYVLTHREDEQVNRKAEQIALGLTVGSWTDLPQLKKEQLQKHKGRVEKVTEKFAPAENGLYQSEVTIAYPEANFSADIPAVLSTIFGKLSLDGKVKLVDIQFSDRFKKSLPGPVFGIDGIRKKTGVFDRPLLMSIFKGVIGRDMTDLKEQLRLQALGGVDFIKDDEILFESPLAPFEDRIKEGKKILKETYEETGHRTLYAVHLTGRTFELRDRARKAAELGADALLFNVFAYGLDVMQSLAEDPDIRLPIMAHPAVSGALTSSPEYGFSHSLLLGKLNRYAGADLSLFPSPYGSVALPKKDAFGIYEACVKEDSVQKTFPVPSAGIHPGMVPVLMKDFGLDHVINAGGGIHGHPRGAIGGGKAFRSIIDAVIHGESIQEKTASCQDLKAALELWGRVVE | ||||||
Modified residue | 169 | N6-carboxylysine | ||||
Sequence: K |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length406
- Mass (Da)44,486
- Last updated2008-12-16 v2
- ChecksumE9E183E7C681E4ED
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000813 EMBL· GenBank· DDBJ | ABV61935.2 EMBL· GenBank· DDBJ | Genomic DNA |