A8E1C4 · LTP_MUHVK
- ProteinLarge tegument protein deneddylase
- GeneM48
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids2147 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Large tegument protein that plays multiple roles in the viral cycle. During viral entry, remains associated with the capsid while most of the tegument is detached and participates in the capsid transport toward the host nucleus. Plays a role in the routing of the capsid at the nuclear pore complex and subsequent uncoating. Within the host nucleus, acts as a deneddylase and promotes the degradation of nuclear CRLs (cullin-RING ubiquitin ligases) and thereby stabilizes nuclear CRL substrates, while cytoplasmic CRLs remain unaffected. These modifications prevent host cell cycle S-phase progression and create a favorable environment allowing efficient viral genome replication. Participates later in the secondary envelopment of capsids. Indeed, plays a linker role for the association of the outer viral tegument to the capsids together with the inner tegument protein.
Catalytic activity
Features
Showing features for site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 10 | Important for catalytic activity | ||||
Sequence: Q | ||||||
Active site | 23 | |||||
Sequence: C | ||||||
Active site | 156 | |||||
Sequence: D | ||||||
Active site | 158 | |||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell cytoplasm | |
Cellular Component | host cell nucleus | |
Cellular Component | viral tegument | |
Molecular Function | cysteine-type deubiquitinase activity | |
Biological Process | proteolysis | |
Biological Process | symbiont-mediated perturbation of host ubiquitin-like protein modification |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLarge tegument protein deneddylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Peploviricota > Herviviricetes > Herpesvirales > Orthoherpesviridae > Betaherpesvirinae > Muromegalovirus > Muromegalovirus muridbeta1 > Murid herpesvirus 1
- Virus hosts
Accessions
- Primary accessionA8E1C4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Tightly associated with the capsid.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000396954 | 1-2147 | Large tegument protein deneddylase | |||
Sequence: MKIVRASRDQSAPVYGPRAGSQCMSNCFTFLHTCYLMGIDPVLDTTSLDAVLDSGARLDAIADEKVKRQALTDHPYRLGTEIPTVIETPAGITGHALSRPFNGTAETQDLGGYKCLGILDFLTYARGKPLPVYIIVTVGVHTRGVIVARGATYVFDPHTTDLSAEAAVYVCDDFTEAISALSFFGAMIGDFYYDAVLVYFTRCRTTLISPSELLVQIMDQYKDPDIDASVMSGSGGGGPSISSSAASASASVSPLPSGAASDGVTNGAGAQTSSKSGKKRRAPDVPPQQKGTAKTLRRSRRPIRLPERLSEVIVEDVQTIEHFRTATQSLPVKPPQEWTMYCGEEPFYRQYFVDVGRQLLAHAIDTYVSLDKTTDESAVQRFESLMGLSDELDAVIAAARATELSAPPLYKTYLSQRVSASAVTRTDRLLASKILALFEEGSATDFETVSSWLRELLQQLPVENTTTTEAKIAAFVAQHPIPGERAFVCLRNSQVKSLGELLSVKRETLKVKYMENDATYRKILDAISKLGLASNAAAAIQSADTSHLDSEQLASLAQAAEAYAQSAYESCQAKMTELLSTNHNRILTGSLPDSDIAAVLAAFKAVSENVRALREVELLKSQVHVQLAQLTEDLLYLQTAEVKLEVTPSAEIKKLRAEYETARKQISDEEMRIKELIENMEDMITDSSSSPPPPEMLDMLRTQIEEVESMTVDEQDARRADKVLGKLSTLDEAEAKATEFAQSLSTVNIPSLNEIKGVKMLKSVLETNADLRRAYVQAVTGMLENALKQLAEGNLPSDDMMSRITALAEQLPTGRQVRADLLDSTDIVSQMSRRLRYAANQKNSTQSLEDVLNFFSENDDMIRKLLKTSWGKPVATVYRRVQVEYDRKMEELRESEWLKRVKETDIDSPQTLERLLKTAPNETILAKHAPDMHARLKKRMQSEAEKRTADMKRLYEEMRKKVDTDLKVVSDSFSSQTPSMFSSIDLKSCGTSLVRLTKEDRKAAIATFNANLTKSLNSLLASLVTVETATIAAILKGADPEATSSTGSAGGGADRQKHTSTLENNISTLLGWQQKVLLPETERDLLTIAHLLTALTHMRKNWRNPAAAFSSTPHTAAYRNFAELANEIESRRTETLARYKSKYAELNASIHDNTKANDVTIKPEDTFTAAFRDTVKAMAAPFSTELQARLQARENELKDELSDLNVKLKTKLERYLAKRSSQDARWRDLITQHRIRLPEGLDVDTNRLRTDTVVTLNGIIRSAATTLPYITAKRGLEWTTEFILAAIEEKKDADPGMFAQLATLLDNSQAIARKIEEKIVYNTKVEAEMLETAPEHTRESLSNLQLMLGQLEAKRVVGGEARYKILSDAILTKQNKLAFAEDLEKLSARYFELARDIRSSKYGLDFDAQLLKTSLLKSEIGQHKKDPPSTGEEVGLPEESTTAAKISISSLLLGIAALEKYIVAHRTLLDNFVSSQPLISQAENIPALVGGPGEDDKPGQEPFDWTRIDLSRLSACDVSTALYRGTDVFGERRVMTARGIQLYLYATHGNFVFEAFSHVRGTKGLLSSSSGGGNGSGASSRQKDQQNATVTRRYRSVTVLASIAATLQTFWSEISRYDIRELLVESGRDPESAADQRDRRLNTVMNLKLFVYVMTVAWSEATPPVEPGSPEATHALEVSLLDFSTLMAALHPEYVYAITTQPVDATLRGLIARLDRRTVDAAMNTQENPPPYDMRELKAFCLDTKQWTQEDIRPQMWMSDLVKQICTSHPRNRDASTSTKLFLYMLATKVLPRDILRCLWVQFRPAYASSIASLEELVSALCDSFFKIYGTTSETVSARLKTGEKVERQVVLRHKPTMSLLDEFSQQEAVLDYILGSYVFAIPMTVGIHVTNIVNGRYRLIVRHLENLPSDPDFVKVVRSRDLSFDRFGWSYTVQNPVERSWFSLQEDRLRHLLTNPPQQDRTPLVVYDSNTNYAVNAMMPPLKAPPATSRVHLTVKNPFSTMRMIPHEDEGDDAATSETPFTSLPINIDFLRRDPPRLKRASGDSSSSVPAEDRDPDARPQDSVPDQSLSEPLFSQTKVSSIVPKDAVTTLSNESISQTFQIHPFRALSSAIMAAIEILQETRLQLDTFESDMCEAIRRIKILYLH |
Interaction
Subunit
Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins. Interacts with inner tegument protein. Interacts with capsid vertex specific component CVC2. Interacts with the major capsid protein/MCP.
Protein-protein interaction databases
Family & Domains
Features
Showing features for region, domain, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-233 | Deubiquitination activity | ||||
Sequence: MKIVRASRDQSAPVYGPRAGSQCMSNCFTFLHTCYLMGIDPVLDTTSLDAVLDSGARLDAIADEKVKRQALTDHPYRLGTEIPTVIETPAGITGHALSRPFNGTAETQDLGGYKCLGILDFLTYARGKPLPVYIIVTVGVHTRGVIVARGATYVFDPHTTDLSAEAAVYVCDDFTEAISALSFFGAMIGDFYYDAVLVYFTRCRTTLISPSELLVQIMDQYKDPDIDASVMSG | ||||||
Domain | 3-223 | Peptidase C76 | ||||
Sequence: IVRASRDQSAPVYGPRAGSQCMSNCFTFLHTCYLMGIDPVLDTTSLDAVLDSGARLDAIADEKVKRQALTDHPYRLGTEIPTVIETPAGITGHALSRPFNGTAETQDLGGYKCLGILDFLTYARGKPLPVYIIVTVGVHTRGVIVARGATYVFDPHTTDLSAEAAVYVCDDFTEAISALSFFGAMIGDFYYDAVLVYFTRCRTTLISPSELLVQIMDQYKD | ||||||
Region | 228-301 | Disordered | ||||
Sequence: ASVMSGSGGGGPSISSSAASASASVSPLPSGAASDGVTNGAGAQTSSKSGKKRRAPDVPPQQKGTAKTLRRSRR | ||||||
Compositional bias | 235-276 | Polar residues | ||||
Sequence: GGGGPSISSSAASASASVSPLPSGAASDGVTNGAGAQTSSKS | ||||||
Region | 292 | Interaction with inner tegument protein | ||||
Sequence: T | ||||||
Region | 1419-1438 | Disordered | ||||
Sequence: EIGQHKKDPPSTGEEVGLPE | ||||||
Region | 1567-1589 | Disordered | ||||
Sequence: SSSSGGGNGSGASSRQKDQQNAT | ||||||
Region | 2034-2072 | Disordered | ||||
Sequence: RDPPRLKRASGDSSSSVPAEDRDPDARPQDSVPDQSLSE |
Sequence similarities
Belongs to the herpesviridae large tegument protein family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,147
- Mass (Da)238,457
- Last updated2007-11-13 v1
- Checksum4C5DB3F99EF1A63A
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 235-276 | Polar residues | ||||
Sequence: GGGGPSISSSAASASASVSPLPSGAASDGVTNGAGAQTSSKS |
Keywords
- Technical term