A8B4Q0 · TRM5_GIAIC
- ProteintRNA (guanine(37)-N(1))-methyltransferase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids447 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Catalytic activity
- guanosine37 in tRNA + S-adenosyl-L-methionine = N1-methylguanosine37 in tRNA + S-adenosyl-L-homocysteine + H+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 241 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 285-286 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 313-314 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial matrix | |
Cellular Component | nucleus | |
Molecular Function | tRNA (guanine(37)-N1)-methyltransferase activity | |
Biological Process | tRNA methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (guanine(37)-N(1))-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metamonada > Diplomonadida > Hexamitidae > Giardiinae > Giardia
Accessions
- Primary accessionA8B4Q0
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Predominantly in the mitochondria and in the nucleus.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000414157 | 1-447 | tRNA (guanine37-N1)-methyltransferase | ||
Interaction
Structure
Sequence
- Sequence statusComplete
- Length447
- Mass (Da)50,511
- Last updated2007-11-13 v1
- ChecksumA88DBE14CEC869FB
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AACB02000002 EMBL· GenBank· DDBJ | EDO81986.1 EMBL· GenBank· DDBJ | Genomic DNA |