A7J3A3 · VP2_ROTYO

Function

function

Inner capsid protein that self-assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nascent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels formed by VP2 N-termini. VP2 is required for the replicase activity of VP1 polymerase. Probably recruits a copy of a VP1-VP3 complex, potentially along with a segment of plus-strand RNA, as a decamer of VP2 assembles. May activate the autoinhibited VP1/RNA complex to coordinate packaging and genome replication.

Features

Showing features for site.

1890100200300400500600700800
TypeIDPosition(s)Description
Site232Interaction with the intermediate capsid protein VP6
Site236Interaction with the intermediate capsid protein VP6
Site849Interaction with the intermediate capsid protein VP6
Site851Interaction with the intermediate capsid protein VP6

GO annotations

AspectTerm
Cellular ComponentT=2 icosahedral viral capsid
Cellular Componentviral inner capsid
Cellular Componentviral nucleocapsid
Molecular FunctionRNA binding

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Inner capsid protein VP2

Organism names

Accessions

  • Primary accession
    A7J3A3

Subcellular Location

Virion
Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003680591-890Inner capsid protein VP2

Post-translational modification

Sumoylated with SUMO1 and SUMO2. Sumoylation of viral proteins seems to have a positive role on viral replication.

Keywords

Interaction

Subunit

Homodecamer; each decamer is made up of two conformers of VP2, called VP2A and VP2B. Interacts with a VP1-VP3 complex. Interacts with the intermediate capsid protein VP6. Interacts with NSP5. Interacts (via N-terminus) with NSP2.

Structure

3D structure databases

Family & Domains

Features

Showing features for compositional bias, region.

Type
IDPosition(s)Description
Compositional bias1-30Basic and acidic residues
Region1-46Disordered
Region1-885-fold hub; involved in the encapsidation of VP1 and VP3
Compositional bias31-45Polar residues
Region404-424Hydrophobic
Region432-452Hydrophobic

Domain

The N-terminus binds RNA. It is necessary for encapsidation of VP1 and VP3. The N-termini of 10 VP2 molecules form a cylindrical hub underneath each 5-fold axis of the inner capsid.

Sequence similarities

Belongs to the rotavirus VP2 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    890
  • Mass (Da)
    103,927
  • Last updated
    2007-09-11 v1
  • Checksum
    EEAA4BD3C1586DC1
MAYRKRGVKRENLPQQHERLQEKEIENNTDVTMENKNNNRKQQLSDKVLSQKEEIITDVQDDIKIADEVKKSSKEESKQLLEILKTKEDHQKEVQYEILQKTIPTFEPKESILKKLEDIRPEQAKKQMKLFRIFEPRQLPIYRTNGEKELRNRWYWKLKKDTLPDGDYDVREYFLNLYDQILIEMPDYLLLKDMAVENKNSRDAGKVVDSETANICDAIFQDEETEGVIRRFIADMRQQVQADRNIVNYPSILHPIDHAFNEYFLNHQLVEPLNNEIIFNYIPERIRNDVNYILNMDMNLPSTARYIRPNLLQDRLNLHDNFESLWDTITTSNYILARSVVPDLKEKELVSTEAQIQKMSQDLQLEALTIQSETQFLAGINSQAANDCFKTLIAAMLSQRTMSLDFVTTNYMSLISGMWLLTVIPNDMFLRESLVACELAIINTIVYPAFGMQRMHYRNGDPQTPFQIAEQQIQNFQVANWLHFINNNRFRQVVIDGVLNQTLNDNIRNGQVINQLMEALMQLSRQQFPTMPVDYKRSIQRGILLLSNRLGQLVDLTRLVSYNYETLMACITMNMQHVQTLTTEKLQLTSVTSLCMLIGNTTVIPSPQTLFHYYNVNVNFHSNYNERINDAVAIITAANRLNLYQKKMKSIVEDFLKRLQIFDVPRVPDDQMYRLRDRLRLLPVERRRLDIFNLILMNMEQIERASDKIAQGVIIAYRDMQLERDEMYGFVNIARNLDGYQQINLEELMRTGDYGQITNMLLNNQPVALVGALPFVTDSSVISLIAKLDATVFAQIVKLRKVDTLKPILYKINSDSNDFYLVANYDWIPTSTTKVYKQVPQPFDFRASMHMLTSNLTFTVYSDLLSFVSADTVEPINAIAFDNMRIMNEL

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-30Basic and acidic residues
Compositional bias31-45Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DQ870498
EMBL· GenBank· DDBJ
ABI60853.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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